BACKGROUND Unexplained infertility(UI)is usually used for any heterosexual couple who failed to have a successful clinical pregnancy without identifying clear causes after they undergo all standard fertility tests.Evi...BACKGROUND Unexplained infertility(UI)is usually used for any heterosexual couple who failed to have a successful clinical pregnancy without identifying clear causes after they undergo all standard fertility tests.Evidence shows that leptin is one of the most accurate biomarkers for UI.Nevertheless,conflicting results regarding leptin levels in women with UI have been reported.AIM To find the serum leptin levels in women with UI.METHODS All studies written in English and conducted before April 30,2021 from PubMed/MEDLINE,Embase,Cochrane Central Register of Controlled Trials,ClinicalTrials.gov,Google Scholar,OpenGrey,OATD,and the infertility conference abstract were included.Studies were found eligible if they provided the mean and standard deviation of leptin for the case group and control group.The quality assessment of individual studies was evaluated using the Joanna Briggs Institute Quality Assessment Tool.Data synthesis and statistical analysis were done using STATA software version 16.RESULTS A total of 378 studies were reviewed,and just six studies that fulfilled the eligibility criteria were included in this meta-analysis.The pooled result showed that leptin levels were significantly higher in women with UI compared to fertile women,with a standardized mean difference of 0.97(95%confidence interval:-0.49-2.43).However,heterogeneity across studies was highly significant(P<0.00001;I2=98.8%).CONCLUSION The results of this study suggest that leptin levels are elevated in women with UI compared with fertile women;hence,leptin could be a potential biomarker for UI in women,and it may be useful for identifying women with a high risk of infertility.展开更多
BACKGROUND The excessive concentration of leptin has negative effects on all aspects of female reproduction.Despite this established relationship,the exact role of leptin in women’s fertility is not clear enough and ...BACKGROUND The excessive concentration of leptin has negative effects on all aspects of female reproduction.Despite this established relationship,the exact role of leptin in women’s fertility is not clear enough and needs more clarification.AIM To evaluate the serum leptin levels in Sudanese women and to ascertain the relationship between serum leptin levels and unexplained infertility(UI).METHODS A matched(age and body mass index)case-control study was conducted from March 2021 to February 2022.The study samples were 210 women with UI and 190 fertile women of reproductive age who were attending the maternity hospitals and fertility clinics in Khartoum state Sudan.The serum concentration of leptin and other serum biomarkers were determined using enzyme-linked immunosorbent assays.RESULTS The results showed that there was a highly statistically significant difference between the two groups(P<0.001)for all examined eight biomarkers.Whereby,leptin,luteinizing hormone(LH)/follicular stimulating hormone(FSH)ratio,prolactin hormone(PRL)and testosterone(T)were significantly higher in the UI group compared with the control group.In contrast,FSH and estradiol(E2)/T ratio were significantly lower in the UI group than in the control group and the effect size test for the difference between the two groups was very large(effect size>0.80),for leptin level,LH/FSH ratio,PRL level,and E2/T ratio,and large(effect size 0.50-≤0.80)for FSH and T.CONCLUSION This study reveals that leptin could be a potential biomarker for UI in Sudanese women and it may be useful for identifying women with a high risk of infertility.展开更多
目的分析糖尿病孕妇血清载脂蛋白M(apolipoprotein M,ApoM)、瘦素、胰岛素及血脂水平变化特征及联动变化关系。方法选取作者医院2016-03/2018-05月孕中期(孕24～28周)糖尿病孕妇80例,孕晚期(分娩前1周)糖尿病孕妇110例,选取同孕期正常...目的分析糖尿病孕妇血清载脂蛋白M(apolipoprotein M,ApoM)、瘦素、胰岛素及血脂水平变化特征及联动变化关系。方法选取作者医院2016-03/2018-05月孕中期(孕24～28周)糖尿病孕妇80例,孕晚期(分娩前1周)糖尿病孕妇110例,选取同孕期正常孕妇为对照组(正常对照1组,n=90;正常对照2组,n=90)。测定血清中ApoM、瘦素、胰岛素及血脂水平,观察各生化指标水平变化特征,并分析各指标间的相关性。结果孕中期糖尿病孕妇组血清ApoM水平明显低于正常对照1组(P<0.001);孕晚期糖尿病孕妇组血清ApoM水平明显高于正常对照2组(P<0.001)。孕中期糖尿病孕妇组胰岛素、胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)水平明显高于正常对照1组(P均<0.001),而在瘦素上比较无统计学差异(P>0.05);孕晚期糖尿病孕妇组TC、TG、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、LDL-C水平明显高于正常对照2组(P<0.01或0.05),而在胰岛素和瘦素上比较差异无统计学意义(P>0.05)。孕中期与孕晚期糖尿病孕妇的血清ApoM水平与胰岛素、瘦素、TC、TG均具有相关性(r=0.246～0.317,P<0.05),与HDL-C、LDL-C无关。结论糖尿病孕妇血清ApoM表达异常,ApoM可能在妊娠期糖尿病的病理生理过程中发挥作用,且ApoM水平与胰岛素、瘦素、TC、TG均具有相关性。展开更多
下丘脑在小型哺乳动物能量稳态的维持中发挥着重要的作用,它是能量代谢平衡的调控中枢,下丘脑神经肽相互作用形成的调控网络可以调节动物的能量稳态。为阐明高山姬鼠下丘脑神经肽基因表达量在低温条件下对其体重调节的作用,在冷驯化条...下丘脑在小型哺乳动物能量稳态的维持中发挥着重要的作用,它是能量代谢平衡的调控中枢,下丘脑神经肽相互作用形成的调控网络可以调节动物的能量稳态。为阐明高山姬鼠下丘脑神经肽基因表达量在低温条件下对其体重调节的作用,在冷驯化条件下测定了高山姬鼠的体重、体脂含量、食物摄入量、血清瘦素浓度以及神经肽Y(neuropeptide Y,NPY)、刺鼠相关蛋白(agouti related peptide,AgRP)、阿片促黑色素原(pro-opiomelanocortin,POMC)和可卡因-安他非明转录调节肽(cocaine and amphetamine regulated transcript peptide,CART)编码基因的表达量。结果表明:低温降低高山姬鼠的体重和体脂,增加摄食量;对照组和低温组的血清瘦素含量差异显著(P<0.05),瘦素含量与脂肪含量呈正相关关系;对照组和低温组的下丘脑神经肽基因NPY和AgRP的表达量差异极显著(P<0.01),而POMC和CART的表达量差异不显著(P>0.05)。以上结果表明低温可以降低高山姬鼠的体重、体脂和瘦素含量,增加摄食量、NPY和AgRP的表达量。此外,低温条件下高山姬鼠可能通过调整下丘脑神经肽NPY和AgRP基因表达量来调节其体重。展开更多
文摘BACKGROUND Unexplained infertility(UI)is usually used for any heterosexual couple who failed to have a successful clinical pregnancy without identifying clear causes after they undergo all standard fertility tests.Evidence shows that leptin is one of the most accurate biomarkers for UI.Nevertheless,conflicting results regarding leptin levels in women with UI have been reported.AIM To find the serum leptin levels in women with UI.METHODS All studies written in English and conducted before April 30,2021 from PubMed/MEDLINE,Embase,Cochrane Central Register of Controlled Trials,ClinicalTrials.gov,Google Scholar,OpenGrey,OATD,and the infertility conference abstract were included.Studies were found eligible if they provided the mean and standard deviation of leptin for the case group and control group.The quality assessment of individual studies was evaluated using the Joanna Briggs Institute Quality Assessment Tool.Data synthesis and statistical analysis were done using STATA software version 16.RESULTS A total of 378 studies were reviewed,and just six studies that fulfilled the eligibility criteria were included in this meta-analysis.The pooled result showed that leptin levels were significantly higher in women with UI compared to fertile women,with a standardized mean difference of 0.97(95%confidence interval:-0.49-2.43).However,heterogeneity across studies was highly significant(P<0.00001;I2=98.8%).CONCLUSION The results of this study suggest that leptin levels are elevated in women with UI compared with fertile women;hence,leptin could be a potential biomarker for UI in women,and it may be useful for identifying women with a high risk of infertility.
基金the Pan African University of Life and Earth Sciences Institutethe African Union for financial supportthe University of Ibadan for hosting this Ph D program
文摘BACKGROUND The excessive concentration of leptin has negative effects on all aspects of female reproduction.Despite this established relationship,the exact role of leptin in women’s fertility is not clear enough and needs more clarification.AIM To evaluate the serum leptin levels in Sudanese women and to ascertain the relationship between serum leptin levels and unexplained infertility(UI).METHODS A matched(age and body mass index)case-control study was conducted from March 2021 to February 2022.The study samples were 210 women with UI and 190 fertile women of reproductive age who were attending the maternity hospitals and fertility clinics in Khartoum state Sudan.The serum concentration of leptin and other serum biomarkers were determined using enzyme-linked immunosorbent assays.RESULTS The results showed that there was a highly statistically significant difference between the two groups(P<0.001)for all examined eight biomarkers.Whereby,leptin,luteinizing hormone(LH)/follicular stimulating hormone(FSH)ratio,prolactin hormone(PRL)and testosterone(T)were significantly higher in the UI group compared with the control group.In contrast,FSH and estradiol(E2)/T ratio were significantly lower in the UI group than in the control group and the effect size test for the difference between the two groups was very large(effect size>0.80),for leptin level,LH/FSH ratio,PRL level,and E2/T ratio,and large(effect size 0.50-≤0.80)for FSH and T.CONCLUSION This study reveals that leptin could be a potential biomarker for UI in Sudanese women and it may be useful for identifying women with a high risk of infertility.
文摘目的分析糖尿病孕妇血清载脂蛋白M(apolipoprotein M,ApoM)、瘦素、胰岛素及血脂水平变化特征及联动变化关系。方法选取作者医院2016-03/2018-05月孕中期(孕24～28周)糖尿病孕妇80例,孕晚期(分娩前1周)糖尿病孕妇110例,选取同孕期正常孕妇为对照组(正常对照1组,n=90;正常对照2组,n=90)。测定血清中ApoM、瘦素、胰岛素及血脂水平,观察各生化指标水平变化特征,并分析各指标间的相关性。结果孕中期糖尿病孕妇组血清ApoM水平明显低于正常对照1组(P<0.001);孕晚期糖尿病孕妇组血清ApoM水平明显高于正常对照2组(P<0.001)。孕中期糖尿病孕妇组胰岛素、胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)水平明显高于正常对照1组(P均<0.001),而在瘦素上比较无统计学差异(P>0.05);孕晚期糖尿病孕妇组TC、TG、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、LDL-C水平明显高于正常对照2组(P<0.01或0.05),而在胰岛素和瘦素上比较差异无统计学意义(P>0.05)。孕中期与孕晚期糖尿病孕妇的血清ApoM水平与胰岛素、瘦素、TC、TG均具有相关性(r=0.246～0.317,P<0.05),与HDL-C、LDL-C无关。结论糖尿病孕妇血清ApoM表达异常,ApoM可能在妊娠期糖尿病的病理生理过程中发挥作用,且ApoM水平与胰岛素、瘦素、TC、TG均具有相关性。
文摘下丘脑在小型哺乳动物能量稳态的维持中发挥着重要的作用,它是能量代谢平衡的调控中枢,下丘脑神经肽相互作用形成的调控网络可以调节动物的能量稳态。为阐明高山姬鼠下丘脑神经肽基因表达量在低温条件下对其体重调节的作用,在冷驯化条件下测定了高山姬鼠的体重、体脂含量、食物摄入量、血清瘦素浓度以及神经肽Y(neuropeptide Y,NPY)、刺鼠相关蛋白(agouti related peptide,AgRP)、阿片促黑色素原(pro-opiomelanocortin,POMC)和可卡因-安他非明转录调节肽(cocaine and amphetamine regulated transcript peptide,CART)编码基因的表达量。结果表明:低温降低高山姬鼠的体重和体脂,增加摄食量;对照组和低温组的血清瘦素含量差异显著(P<0.05),瘦素含量与脂肪含量呈正相关关系;对照组和低温组的下丘脑神经肽基因NPY和AgRP的表达量差异极显著(P<0.01),而POMC和CART的表达量差异不显著(P>0.05)。以上结果表明低温可以降低高山姬鼠的体重、体脂和瘦素含量,增加摄食量、NPY和AgRP的表达量。此外,低温条件下高山姬鼠可能通过调整下丘脑神经肽NPY和AgRP基因表达量来调节其体重。
