研究了三角帆蚌(Hyriopsis cum ingiiLea)对藻类的滤食和消化能力。通过对三角帆蚌生活的池塘以及三角帆蚌的胃、中肠、直肠中所含藻类的鉴定和含量的对比发现,三角帆蚌能滤食环境中大部分的藻类;对于滤取到消化道中的藻类,除一些结构...研究了三角帆蚌(Hyriopsis cum ingiiLea)对藻类的滤食和消化能力。通过对三角帆蚌生活的池塘以及三角帆蚌的胃、中肠、直肠中所含藻类的鉴定和含量的对比发现,三角帆蚌能滤食环境中大部分的藻类;对于滤取到消化道中的藻类,除一些结构复杂的外,都能很好的消化。其中,蓝藻门的总消化率为78.8%,消化度为+4;硅藻门的总消化率为90.0%,消化度为+5;隐藻门的总消化率为41.1%,消化度为+3;裸藻门的总消化率为46.5%,消化度为+3;绿藻门的总消化率为49.1%,消化度为+3。研究结果为利用滤食性贝类治理富营养化水体中的水华污染提供了可行的路径。展开更多
The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the prese...The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the present work, a cDNA encoding DREB1/CBF-like protein (GhDREB1L) from cotton was isolated, and its sequence features, DNA binding preference, and expression patterns of the transcripts were also characterized. GhDREB1L contained one conserved AP2/ERF domain and its amino acid sequence was similar to the DREB1/CBF group of the DREB family from other plants. The DNA-binding domain of GhDREB1L was successfully expressed as a fusion protein in Escherichia coli BL21 (DE3) and purified by Ni-NTA affinity chromatography. Electrophoretic mobility shift assay revealed that the purified GhDREB1L fusion protein had a specific binding activity with the previously characterized DRE ele-ment (core sequence, ACCGAC) and also with the DRE-like sequence (core sequence, GCCGAC) in the promoter of the dehydration-responsive late embryogenesis-abundant gene LEA D113. Semi-quantita- tive RT-PCR showed that GhDREB1L was induced in the cotton cotyledons by low temperature, as well as drought and NaCl treatments. These results suggested that the novel cotton GhDREB1L might play an important role in response to low temperature as well as drought and high salinity through binding to the DRE cis-element.展开更多
Abstract: In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were performed using an Escherichia coli heterologous expression system. Three soybean late embryoge...Abstract: In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were performed using an Escherichia coli heterologous expression system. Three soybean late embryogenesis abundant (LEA) genes, PM11 (GenBank accession No. AF004805; group 1), PM30 (AF117884; group 3), and ZLDE-2 (AY351918; group 2), were cloned and expressed in a pET-28a system. The gene products were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by mass spectrometry. E. coli cells containing the recombinant plasmids or empty vector as controls were treated by salt and low temperature stress. Compared with control cells, the E. coli cells expressing either PM11 or PM30 showed a shorter lag period and improved growth when transferred to LB (Luria-Bertani) liquid media containing 800 mmol/L NaCl or 700 mmol/L KCl or after 4°C treatment. E. coli cells expressing ZLDE-2 did not show obvious growth improvement both in either high KCl medium or after 4°C treatment. The results indicate that the E. coli expression system is a simple, useful method to identify the functions of some stress-tolerant genes from plants.展开更多
The group 3 late embryogenesis abundant （LEA） proteins are thought to protect cells from stresses associated with dehydration during periods of water deficit. To investigate the functions of different members of the...The group 3 late embryogenesis abundant （LEA） proteins are thought to protect cells from stresses associated with dehydration during periods of water deficit. To investigate the functions of different members of the group 3 LEA genes, we isolated and characterized two new group 3 LEA genes, namely TaLEA2 and TaLEA3, from wheat （Triticum aestivum L.） and introduced TaLEA2 and TaLEA3 into Saccharmyces cerevisiae to examine the effect of these genes on yeast cell tolerance to osmotic, salt, and cold stresses. The TaLEA2 gene encoded a protein of 211 amino acids and possessed five repeats of 11-mer amino acid motifs. The TaLEA3 gene encoded a polypeptide of 211 amino acids with nine repeated units. Overexpression of TaLEA2 and TaLEA3 improved stress tolerance in transgenic yeast cells when cultured in medium containing sorbitol, salt and-20℃ freezing treatments respectively. However, the yeast transformants with TaLEA2 seemed to be more tolerant to hyperosmotic and freezing stress than transformants with TaLEA3. This implies that a close relationship exists between function and the number of repeats of the 11- mer amino acid motif in the group 3 LEA protein.展开更多
The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V ...The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.展开更多
The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezi...The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezing stresses and additional tolerance to heat, NaHCO3 （salt-alkali） and ultraviolet radiation was also investigated. The transgenic yeast harboring the Tamarix LEA gene （DQ663481） was generated under the control of inducible GAL promoter （pYES2 vector）, yeast cells transformed with pYES2 empty vector were also generated as a control. Stress tolerance tests showed that LEA yeast transformants exhibited a higher survival rates than the control transformants under high temperature, NaHCO3, ultraviolet radiation, salt （NaCl）, drought and freezing, indicating that the LEA gene is tolerant to these abiotic stresses. These results suggest that the LEA gene is resistant to a wider repertoire of stresses and may play a common role in plant acclimation to the examined stress conditions.展开更多
Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific prot...Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.展开更多
文摘研究了三角帆蚌(Hyriopsis cum ingiiLea)对藻类的滤食和消化能力。通过对三角帆蚌生活的池塘以及三角帆蚌的胃、中肠、直肠中所含藻类的鉴定和含量的对比发现,三角帆蚌能滤食环境中大部分的藻类;对于滤取到消化道中的藻类,除一些结构复杂的外,都能很好的消化。其中,蓝藻门的总消化率为78.8%,消化度为+4;硅藻门的总消化率为90.0%,消化度为+5;隐藻门的总消化率为41.1%,消化度为+3;裸藻门的总消化率为46.5%,消化度为+3;绿藻门的总消化率为49.1%,消化度为+3。研究结果为利用滤食性贝类治理富营养化水体中的水华污染提供了可行的路径。
基金the State Key Basic Research and Development Plan of China (Grant No. 2004CB117303),the Hi-Tech Research and Development Program of China (Grant Nos.2004AA222100, 2002AA212051 and 2002AA207006),the National Natural Science Foundation of China (Grant Nos. 30170080 and 39770078)
文摘The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the present work, a cDNA encoding DREB1/CBF-like protein (GhDREB1L) from cotton was isolated, and its sequence features, DNA binding preference, and expression patterns of the transcripts were also characterized. GhDREB1L contained one conserved AP2/ERF domain and its amino acid sequence was similar to the DREB1/CBF group of the DREB family from other plants. The DNA-binding domain of GhDREB1L was successfully expressed as a fusion protein in Escherichia coli BL21 (DE3) and purified by Ni-NTA affinity chromatography. Electrophoretic mobility shift assay revealed that the purified GhDREB1L fusion protein had a specific binding activity with the previously characterized DRE ele-ment (core sequence, ACCGAC) and also with the DRE-like sequence (core sequence, GCCGAC) in the promoter of the dehydration-responsive late embryogenesis-abundant gene LEA D113. Semi-quantita- tive RT-PCR showed that GhDREB1L was induced in the cotton cotyledons by low temperature, as well as drought and NaCl treatments. These results suggested that the novel cotton GhDREB1L might play an important role in response to low temperature as well as drought and high salinity through binding to the DRE cis-element.
基金the National Special Program for Research and Industrialization of Transgenic Plants，国家自然科学基金，Foundation for Key Teachers in Universities
文摘Abstract: In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were performed using an Escherichia coli heterologous expression system. Three soybean late embryogenesis abundant (LEA) genes, PM11 (GenBank accession No. AF004805; group 1), PM30 (AF117884; group 3), and ZLDE-2 (AY351918; group 2), were cloned and expressed in a pET-28a system. The gene products were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by mass spectrometry. E. coli cells containing the recombinant plasmids or empty vector as controls were treated by salt and low temperature stress. Compared with control cells, the E. coli cells expressing either PM11 or PM30 showed a shorter lag period and improved growth when transferred to LB (Luria-Bertani) liquid media containing 800 mmol/L NaCl or 700 mmol/L KCl or after 4°C treatment. E. coli cells expressing ZLDE-2 did not show obvious growth improvement both in either high KCl medium or after 4°C treatment. The results indicate that the E. coli expression system is a simple, useful method to identify the functions of some stress-tolerant genes from plants.
文摘The group 3 late embryogenesis abundant （LEA） proteins are thought to protect cells from stresses associated with dehydration during periods of water deficit. To investigate the functions of different members of the group 3 LEA genes, we isolated and characterized two new group 3 LEA genes, namely TaLEA2 and TaLEA3, from wheat （Triticum aestivum L.） and introduced TaLEA2 and TaLEA3 into Saccharmyces cerevisiae to examine the effect of these genes on yeast cell tolerance to osmotic, salt, and cold stresses. The TaLEA2 gene encoded a protein of 211 amino acids and possessed five repeats of 11-mer amino acid motifs. The TaLEA3 gene encoded a polypeptide of 211 amino acids with nine repeated units. Overexpression of TaLEA2 and TaLEA3 improved stress tolerance in transgenic yeast cells when cultured in medium containing sorbitol, salt and-20℃ freezing treatments respectively. However, the yeast transformants with TaLEA2 seemed to be more tolerant to hyperosmotic and freezing stress than transformants with TaLEA3. This implies that a close relationship exists between function and the number of repeats of the 11- mer amino acid motif in the group 3 LEA protein.
文摘The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.
基金National Key Program on Basic Research and Development of China (G1999016003)
文摘The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezing stresses and additional tolerance to heat, NaHCO3 （salt-alkali） and ultraviolet radiation was also investigated. The transgenic yeast harboring the Tamarix LEA gene （DQ663481） was generated under the control of inducible GAL promoter （pYES2 vector）, yeast cells transformed with pYES2 empty vector were also generated as a control. Stress tolerance tests showed that LEA yeast transformants exhibited a higher survival rates than the control transformants under high temperature, NaHCO3, ultraviolet radiation, salt （NaCl）, drought and freezing, indicating that the LEA gene is tolerant to these abiotic stresses. These results suggest that the LEA gene is resistant to a wider repertoire of stresses and may play a common role in plant acclimation to the examined stress conditions.
文摘Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.
