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鼠李糖乳杆菌GG(LGG)高密度培养基优化 被引量:4
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作者 王智鼎 刘艳秋 +1 位作者 李岳飞 孙德军 《食品科技》 CAS 北大核心 2013年第11期8-11,共4页
鼠李糖乳杆菌GG是国家认可的第3代益生菌的一种,因其可在肠道定植并且有耐酸碱等特性而得到广泛应用。实验希望优化其培养基,使鼠李糖乳杆菌GG能用较低的成本来进行高密度培养。首先以MRS培养基为基础,根据物种特性进行碳源(麦芽糖:葡萄... 鼠李糖乳杆菌GG是国家认可的第3代益生菌的一种,因其可在肠道定植并且有耐酸碱等特性而得到广泛应用。实验希望优化其培养基,使鼠李糖乳杆菌GG能用较低的成本来进行高密度培养。首先以MRS培养基为基础,根据物种特性进行碳源(麦芽糖:葡萄糖:乳糖:蔗糖)、氮源(胰蛋白胨:牛肉膏:酵母粉)和增菌物质(黄瓜汁:西红柿汁)的成分分别调整,之后进行单因素分析。在相同的条件下,经过37℃厌氧、恒温培养16 h后,测OD600来比较增菌效果。实验发现胰蛋白胨、酵母粉、葡萄糖、西红柿汁这4种物质增菌明显。在以上述4种物质设计4因素3水平L9(34)的正交分析,结果为鼠李糖乳杆菌GG影响性西红柿汁>胰蛋白胨>葡萄糖>酵母粉,其中最佳配比方案为A3B1C3D2(2.5%胰蛋白胨+1.5%酵母粉+2.5%葡萄糖+20%西红柿汁)。在优化配方下鼠李糖乳杆菌GG可达9.4×109cfu/mL。 展开更多
关键词 鼠李糖乳杆菌gg(lgg) 高密度培养 正交分析
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Electrospun Food-Grade Ultrafine Fibers from Pectin and Pullulan Blends 被引量:1
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作者 Shih-Chuan Liu Ran Li +2 位作者 Peggy M. Tomasula Ana M. M. Sousa Linshu Liu 《Food and Nutrition Sciences》 2016年第7期636-646,共11页
Electrospinning was used to produce ultrafine fibers and fibrous mats from aqueous solutions containing two edible polysaccharides: pectin (PEC) and pullulan (PUL). The process excluded the use of a synthetic carrier ... Electrospinning was used to produce ultrafine fibers and fibrous mats from aqueous solutions containing two edible polysaccharides: pectin (PEC) and pullulan (PUL). The process excluded the use of a synthetic carrier polymer or non-aqueous solvents thus maintaining the food-grade status of the components. The inclusion of PUL reduced the surface tension and electric conductivity of pectin solution, and promoted molecular entanglement between PEC and PUL as confirmed by rheological analysis. The spinnability of either polysaccharide was promoted by inclusion of the other. Cross-linking PEC networks were obtained by soaking the fibrous mats in successive Ca<sup>2+</sup> solution. The fibrous mats can be used to carry bioactive compounds as demonstrated using probiotic bacteria Lactobacillus rhamnosus GG (LGG) as a model bioactive compound. The research is significant for the development of food products with unique textures and functionalities. 展开更多
关键词 Food Processing Ultrafine Fiber PECTIN PULLULAN lactobacillus rhamnosus gg (lgg) Functional Foods
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