Objective: To evaluate the relationship between chronic kidney dysfunction after transplantation and chronic vascular rejection (CVR), and to evaluate the efficacy and safety of Tanshinone (Tan) and Herba Lysimachiae ...Objective: To evaluate the relationship between chronic kidney dysfunction after transplantation and chronic vascular rejection (CVR), and to evaluate the efficacy and safety of Tanshinone (Tan) and Herba Lysimachiae (Lys) combined with Mycophenolate Mofetil (MMF) to fight against CVR, and to reduce the incidence of chronic dysfunction in rat renal transplantation model. Methods: Sixty-five male SD rats as donors and sixty-five male Wistar rats as recipients were used. The recipients were divided into five Groups, including Group A: Lys+Cyclosporine A (CsA), Group B: Tan+CsA, Group C: MMF+CsA, Group D: CsA, and Group E: normal saline. Kidney function and morphological changes were assessed at 2, 4, and 6 weeks after transplantation. All sections of renal grafts were stained with monoclonal antibodies (McAB), including major histocompability complex class II (OX-6), lymphocyte function antigen-1 (CD11b/CD18), intercellular adhesive molecular-1 (IA29), CD+8 (OX-8), and proliferation cell nuclear antigen (PCNA) (5A10, IgG1k) were used. Results:The two control groups developed typical chronic rejection episodes, and the histologic feature indication of kidney chronic rejection includes loss of renal units and presence of an obliteration arteriopathy involving large renal arteries. These were associated with serum levels of BUN and SCr increased and different degrees of glomerulosclerosis. Their mean survival time was lower than that of other groups. By contrast, serum levels of cytokine in control groups was significantly increased when compared with group B and C (P<0.05). Both group B and C had minimum changes in glomeruli and arteries, and expression levels of PCNA on the glomeruli and tubular cells were higher than those of other groups (P<0.05). However, there was no significant difference (P>0.05) between group B and C. Conclusions: CVR may activate the risk of the factor responsible for the development of graft chronic dysfunction that causes slow, progressive destruction of the transplanted kidney. Tanshinone展开更多
目的建立用高效液相色谱(HPLC)法测定金钱草颗粒中槲皮素和山奈素含量的方法。方法采用Dalian E lite Hy-persil ODS2 5μm(200 mm×4.6 mm)柱,以甲醇-0.4%磷酸溶液(45∶55)为流动相;流速:1.0 m l.m in-1;检测波长为360nm。柱温:30...目的建立用高效液相色谱(HPLC)法测定金钱草颗粒中槲皮素和山奈素含量的方法。方法采用Dalian E lite Hy-persil ODS2 5μm(200 mm×4.6 mm)柱,以甲醇-0.4%磷酸溶液(45∶55)为流动相;流速:1.0 m l.m in-1;检测波长为360nm。柱温:30℃。结果该法线性关系良好,槲皮素平均回收率为101.2%,RSD为1.14%;山奈素平均回收率为100.1%,RSD为1.33%。结论该方法简便、准确、重现性好,可用于该制剂的质量控制和评价。展开更多
文摘Objective: To evaluate the relationship between chronic kidney dysfunction after transplantation and chronic vascular rejection (CVR), and to evaluate the efficacy and safety of Tanshinone (Tan) and Herba Lysimachiae (Lys) combined with Mycophenolate Mofetil (MMF) to fight against CVR, and to reduce the incidence of chronic dysfunction in rat renal transplantation model. Methods: Sixty-five male SD rats as donors and sixty-five male Wistar rats as recipients were used. The recipients were divided into five Groups, including Group A: Lys+Cyclosporine A (CsA), Group B: Tan+CsA, Group C: MMF+CsA, Group D: CsA, and Group E: normal saline. Kidney function and morphological changes were assessed at 2, 4, and 6 weeks after transplantation. All sections of renal grafts were stained with monoclonal antibodies (McAB), including major histocompability complex class II (OX-6), lymphocyte function antigen-1 (CD11b/CD18), intercellular adhesive molecular-1 (IA29), CD+8 (OX-8), and proliferation cell nuclear antigen (PCNA) (5A10, IgG1k) were used. Results:The two control groups developed typical chronic rejection episodes, and the histologic feature indication of kidney chronic rejection includes loss of renal units and presence of an obliteration arteriopathy involving large renal arteries. These were associated with serum levels of BUN and SCr increased and different degrees of glomerulosclerosis. Their mean survival time was lower than that of other groups. By contrast, serum levels of cytokine in control groups was significantly increased when compared with group B and C (P<0.05). Both group B and C had minimum changes in glomeruli and arteries, and expression levels of PCNA on the glomeruli and tubular cells were higher than those of other groups (P<0.05). However, there was no significant difference (P>0.05) between group B and C. Conclusions: CVR may activate the risk of the factor responsible for the development of graft chronic dysfunction that causes slow, progressive destruction of the transplanted kidney. Tanshinone
文摘目的建立用高效液相色谱(HPLC)法测定金钱草颗粒中槲皮素和山奈素含量的方法。方法采用Dalian E lite Hy-persil ODS2 5μm(200 mm×4.6 mm)柱,以甲醇-0.4%磷酸溶液(45∶55)为流动相;流速:1.0 m l.m in-1;检测波长为360nm。柱温:30℃。结果该法线性关系良好,槲皮素平均回收率为101.2%,RSD为1.14%;山奈素平均回收率为100.1%,RSD为1.33%。结论该方法简便、准确、重现性好,可用于该制剂的质量控制和评价。
