目的探讨西红花苷对食管癌细胞细胞增殖、凋亡和侵袭的影响及其可能机制。方法以人食管癌Eca-109细胞为受试对象,采用CCK-8法检测不同浓度西红花苷对细胞的抑制作用,筛选最佳浓度和干预时间。将食管癌Eca-109细胞分为对照组、西红花低...目的探讨西红花苷对食管癌细胞细胞增殖、凋亡和侵袭的影响及其可能机制。方法以人食管癌Eca-109细胞为受试对象,采用CCK-8法检测不同浓度西红花苷对细胞的抑制作用,筛选最佳浓度和干预时间。将食管癌Eca-109细胞分为对照组、西红花低、中、高剂量组,分别按照0μmol/L、17μmol/L、34μmol/L、68μmol/L给予西红花苷干预,采用Transwell侵袭实验检测细胞侵袭能力;Western blot及RT-qPCR法分别检测各组细胞LKB1、AMPK、m TOR mRNA和蛋白表达水平。结果Transwell侵袭和细胞凋亡实验结果表明,与空白对照组比较,低、中、高剂量西红花苷组细胞穿过基膜的数量减少,凋亡率增高,差异有统计学意义(P<0.05或P<0.01);Western blot和RT-qPCR结果显示,与空白对照组比较,低、中、高剂量西红花苷组细胞LKB1、AMPK m RNA和蛋白表达水平升高,mTOR mRNA和蛋白表达水平降低,差异有统计学意义(P<0.05或P<0.01)。结论西红花苷对食管癌可能有抑制作用,其机制可能与调控LKB1/AMPK/mTOR信号通路相关。展开更多
The effect and potential molecular mechanisms of berberine on gluconeogenesis in skeletal muscles and adipose tissues were investigated.After adaptive feeding for one week,8 rats were randomly selected as the normal g...The effect and potential molecular mechanisms of berberine on gluconeogenesis in skeletal muscles and adipose tissues were investigated.After adaptive feeding for one week,8 rats were randomly selected as the normal group and fed on a standard diet.The remaining 32 rats were fed on a high-fat diet and given an intravenous injection of streptozotocin(STZ)for 2 weeks to induce the diabetic models.The diabetic rat models were confirmed by oral glucose tolerance test(OGTT)and randomly divided into 4 groups(n=8 each),which were all fed on a high-fat diet.Berberine(3 g/kg per day)or metformin(183 mg/kg per day)was intragastrically administered to the diabetic rats for 12 weeks,serving as berberine group and metformin group respectively.5-aminoimidazole-4-carboxamide1-β-D-ribofuranoside[AICAR,an agonist of AMP-activated protein kinase(AMPK),0.5 mg/kg per day]was subcutaneously injected to the diabetic rats for 12 weeks,serving as AICAR group.The remaining 8 diabetic rats served as the model group,which was given a 0.5%carboxyl methylcellulose solution by oral gavage.Fasting serum insulin(FINS),OGTT as well as lipid parameters were tested by commercial kit.The protein levels of liver kinase B1(LKB1),AMPK,phosphorylated AMP-activated protein kinase(p-AMPK),transducer of regulated CREB activity 2(TORC2),phosphorylated transducer of regulated CREB activity 2(p-TORC2),phosphoenolpyruvate carboxykinase(PEPCK),and glucose-6-phosphatase(G6Pase)in skeletal muscles and adipose tissues were examined by Western blotting.The results showed that berberine significantly decreased the body weight,plasma glucose,insulin levels,and homeostatic model assessment for insulin resistance(HOMA-IR)of diabetic rats compared with those in the model group.Meanwhile,the serum total triglyceride(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)levels were markedly decreased and high-density lipoprotein cholesterol(HDL-C)level was significantly increased after the treatment with berberine.In addition,we found that berberine si展开更多
文摘目的探讨西红花苷对食管癌细胞细胞增殖、凋亡和侵袭的影响及其可能机制。方法以人食管癌Eca-109细胞为受试对象,采用CCK-8法检测不同浓度西红花苷对细胞的抑制作用,筛选最佳浓度和干预时间。将食管癌Eca-109细胞分为对照组、西红花低、中、高剂量组,分别按照0μmol/L、17μmol/L、34μmol/L、68μmol/L给予西红花苷干预,采用Transwell侵袭实验检测细胞侵袭能力;Western blot及RT-qPCR法分别检测各组细胞LKB1、AMPK、m TOR mRNA和蛋白表达水平。结果Transwell侵袭和细胞凋亡实验结果表明,与空白对照组比较,低、中、高剂量西红花苷组细胞穿过基膜的数量减少,凋亡率增高,差异有统计学意义(P<0.05或P<0.01);Western blot和RT-qPCR结果显示,与空白对照组比较,低、中、高剂量西红花苷组细胞LKB1、AMPK m RNA和蛋白表达水平升高,mTOR mRNA和蛋白表达水平降低,差异有统计学意义(P<0.05或P<0.01)。结论西红花苷对食管癌可能有抑制作用,其机制可能与调控LKB1/AMPK/mTOR信号通路相关。
基金This project was supported by the National Natural Science Foundation of China(No.81673757 and No.81603652).
文摘The effect and potential molecular mechanisms of berberine on gluconeogenesis in skeletal muscles and adipose tissues were investigated.After adaptive feeding for one week,8 rats were randomly selected as the normal group and fed on a standard diet.The remaining 32 rats were fed on a high-fat diet and given an intravenous injection of streptozotocin(STZ)for 2 weeks to induce the diabetic models.The diabetic rat models were confirmed by oral glucose tolerance test(OGTT)and randomly divided into 4 groups(n=8 each),which were all fed on a high-fat diet.Berberine(3 g/kg per day)or metformin(183 mg/kg per day)was intragastrically administered to the diabetic rats for 12 weeks,serving as berberine group and metformin group respectively.5-aminoimidazole-4-carboxamide1-β-D-ribofuranoside[AICAR,an agonist of AMP-activated protein kinase(AMPK),0.5 mg/kg per day]was subcutaneously injected to the diabetic rats for 12 weeks,serving as AICAR group.The remaining 8 diabetic rats served as the model group,which was given a 0.5%carboxyl methylcellulose solution by oral gavage.Fasting serum insulin(FINS),OGTT as well as lipid parameters were tested by commercial kit.The protein levels of liver kinase B1(LKB1),AMPK,phosphorylated AMP-activated protein kinase(p-AMPK),transducer of regulated CREB activity 2(TORC2),phosphorylated transducer of regulated CREB activity 2(p-TORC2),phosphoenolpyruvate carboxykinase(PEPCK),and glucose-6-phosphatase(G6Pase)in skeletal muscles and adipose tissues were examined by Western blotting.The results showed that berberine significantly decreased the body weight,plasma glucose,insulin levels,and homeostatic model assessment for insulin resistance(HOMA-IR)of diabetic rats compared with those in the model group.Meanwhile,the serum total triglyceride(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)levels were markedly decreased and high-density lipoprotein cholesterol(HDL-C)level was significantly increased after the treatment with berberine.In addition,we found that berberine si
