A break-through came from NSFC sponsored research work in photosynthesis of membrane protein: in particular, the research in the crystal structure of the light harvesting protein complex, LHC-Ⅱ.
Cucumber ( Cucumis sativus L.) LHC_Ⅱ complex, which consists of only one subunit (27 kD), was isolated and purified. 2_D crystallization was performed by batch method. The crystal is 0.7 μm×1.0 μm, and diff...Cucumber ( Cucumis sativus L.) LHC_Ⅱ complex, which consists of only one subunit (27 kD), was isolated and purified. 2_D crystallization was performed by batch method. The crystal is 0.7 μm×1.0 μm, and diffracts to 30 ?. The projection map of the negatively stained two_dimensional crystal of LHC_Ⅱ complex shows that the crystal has p3 symmetry, lattice constant 15.4 nm×15.4 nm, which is different from the LHC_Ⅱ of spinach (Spinacia oleracea L.) and pea (Pisum satium L.). A continuous tomographic tilt series, containing 12 projections from the two_dimensional crystal was subjected to 3_D reconstruction. The 3_D model represents that LHC_Ⅱ complex consists of 6 monomers. These trimer and dimer interactions build up the six member ring.展开更多
文摘A break-through came from NSFC sponsored research work in photosynthesis of membrane protein: in particular, the research in the crystal structure of the light harvesting protein complex, LHC-Ⅱ.
文摘Cucumber ( Cucumis sativus L.) LHC_Ⅱ complex, which consists of only one subunit (27 kD), was isolated and purified. 2_D crystallization was performed by batch method. The crystal is 0.7 μm×1.0 μm, and diffracts to 30 ?. The projection map of the negatively stained two_dimensional crystal of LHC_Ⅱ complex shows that the crystal has p3 symmetry, lattice constant 15.4 nm×15.4 nm, which is different from the LHC_Ⅱ of spinach (Spinacia oleracea L.) and pea (Pisum satium L.). A continuous tomographic tilt series, containing 12 projections from the two_dimensional crystal was subjected to 3_D reconstruction. The 3_D model represents that LHC_Ⅱ complex consists of 6 monomers. These trimer and dimer interactions build up the six member ring.
