目的采用HPLC法测定并比较分析藤合欢中山柰苷的量。方法采用甲醇超声处理方法提取藤合欢，HPLC法测定山柰苷的量，色谱柱为Agilent Zorbax Eclipse Plus C18柱（250mm×4．6mm，5dm），甲醇-0．01mol／L磷酸二氢钾溶液-冰醋酸水溶...目的采用HPLC法测定并比较分析藤合欢中山柰苷的量。方法采用甲醇超声处理方法提取藤合欢，HPLC法测定山柰苷的量，色谱柱为Agilent Zorbax Eclipse Plus C18柱（250mm×4．6mm，5dm），甲醇-0．01mol／L磷酸二氢钾溶液-冰醋酸水溶液（40：60：1．5）等度洗脱，体积流量1．0mL／min，检测波长254nm，柱温30℃。结果线性范围在0．0927～0．9267μg（r=0．9998），平均加样回收率为100．8％，RSD为1．1％（n=6）。结论该方法简便、准确，重复性好，可用于藤合欢质量控制；不同产地的藤合欢样本质量存在差异，贮藏时间越久，其所含的黄酮类成分越少。展开更多
The leaves of Bauhinia forficata L. (Fabaceae) are used in Brazilian folk medicine mainly as antidiabetic agent. They are rich in polyphenols, and kaempferitrin and other flavonoids are currently considered as basic c...The leaves of Bauhinia forficata L. (Fabaceae) are used in Brazilian folk medicine mainly as antidiabetic agent. They are rich in polyphenols, and kaempferitrin and other flavonoids are currently considered as basic chemical criteria for the quality and the effectiveness of extracts and phytopharmaceuticals derived from them. Aiming to expand the scope of current data of the chemical profile of B. forficata subsp. forficata, the present study employed HPLC-DAD and HPLC-DAD-ESI/MS to compare the flavonoid profile and kaempferitrin content in the leaves of specimens collected seasonally during one year, in two different locations in Southeastern Brazil: Rio de Janeiro (RJ) and São Paulo (SP) states. The data showed a variation in the flavonoid profile and in the kaempferitrin content depending on the place of collection and the season of the year. The target compound, kaempferitrin, was found to be only a minor constituent in RJ (0.21 - 1.02 μg/mg), and was mostly absent or occurred as trace in SP location (0.00 - 0.12 μg/mg). Some degree of similarity was observed in the flavonoid profiles according to the region of collection. In agreement with some published reports, the low and quite variable kaempferitrin content as well the wide variability of the flavonoid profiles brings into question the dubious matter of using these compounds as chemical markers for this medicinal species.展开更多
文摘目的采用HPLC法测定并比较分析藤合欢中山柰苷的量。方法采用甲醇超声处理方法提取藤合欢，HPLC法测定山柰苷的量，色谱柱为Agilent Zorbax Eclipse Plus C18柱（250mm×4．6mm，5dm），甲醇-0．01mol／L磷酸二氢钾溶液-冰醋酸水溶液（40：60：1．5）等度洗脱，体积流量1．0mL／min，检测波长254nm，柱温30℃。结果线性范围在0．0927～0．9267μg（r=0．9998），平均加样回收率为100．8％，RSD为1．1％（n=6）。结论该方法简便、准确，重复性好，可用于藤合欢质量控制；不同产地的藤合欢样本质量存在差异，贮藏时间越久，其所含的黄酮类成分越少。
文摘The leaves of Bauhinia forficata L. (Fabaceae) are used in Brazilian folk medicine mainly as antidiabetic agent. They are rich in polyphenols, and kaempferitrin and other flavonoids are currently considered as basic chemical criteria for the quality and the effectiveness of extracts and phytopharmaceuticals derived from them. Aiming to expand the scope of current data of the chemical profile of B. forficata subsp. forficata, the present study employed HPLC-DAD and HPLC-DAD-ESI/MS to compare the flavonoid profile and kaempferitrin content in the leaves of specimens collected seasonally during one year, in two different locations in Southeastern Brazil: Rio de Janeiro (RJ) and São Paulo (SP) states. The data showed a variation in the flavonoid profile and in the kaempferitrin content depending on the place of collection and the season of the year. The target compound, kaempferitrin, was found to be only a minor constituent in RJ (0.21 - 1.02 μg/mg), and was mostly absent or occurred as trace in SP location (0.00 - 0.12 μg/mg). Some degree of similarity was observed in the flavonoid profiles according to the region of collection. In agreement with some published reports, the low and quite variable kaempferitrin content as well the wide variability of the flavonoid profiles brings into question the dubious matter of using these compounds as chemical markers for this medicinal species.
