Background： Klebsiellapneumoniae （KP） is a pathogen commonly causing nosocomial infection. Carbapenem-resistant KP （CRKP） is more resistant to multiple antimicrobial drugs than carbapenem-susceptible KP （CSKP） ...Background： Klebsiellapneumoniae （KP） is a pathogen commonly causing nosocomial infection. Carbapenem-resistant KP （CRKP） is more resistant to multiple antimicrobial drugs than carbapenem-susceptible KP （CSKP） isolates. The aim of the present study was to identify the risk factors for CRKP infection and the predictors of mortality among KP-infected adult patients. Methods： Patients with CRKP and CSKP infection were categorized as the case group and control group, respectively, and we conducted a 1：1 ratio case-control study on these groups. The CRKP isolates collected were tested tbr antimicrobial susceptibility and presence of KP carbapenemase （KPC） gene. Clinical data were collected to identity risk factors for CRKP infection and mortality of KP infection. Risk factors were analyzed under univariable and multivariable logistic regression model. Results： The independent risk factors for CRKP infection were admission to Intensive Care Unit （odds ratio [OR]： 15.486, 95% confidence interval [CI]： 3.175-75.541, P 〈 0.001）; use of I3-1actams and [3-1actamase inhibitor combination （OR： 4.765, 95% CI： 1.508-15.055, P = 0.008）： use of cephalosporins （OR： 8.033, 95% CI： 1.623-39.763, P = P= 0.019）： and indwelling of urethral catheter （OR： 6.164, 95% CI： 1.847 0.011 ）; fluoroquinolones （OR： 6.090, 95% CI： 1.343-27.613, -20.578, P = 0.003）. However, older age （OR： 1.079, 95% CI： 1.005-1.158, P= 0.036）, Charlson comorbidity index （OR： 4.690, 95% CI：2.094-10.504, P= 0.000）, and aminoglycoside use （OR： 670.252, 95% CI： 6.577-68,307.730, P = 0.006） were identified as independent risk factors for patient deaths with KP infection. The mortality of CRKP group was higher than that of the CSKP group. KPC gene did not play a role in the CRKP group. CRKP mortality was high. Conclusion： Implementation of infection control measures and protection of the immunefunction are crucial.展开更多
Knowledge of etiology and timely treatment of underlying causes,when possible,play an important role in the successful therapy of patients with pyogenic liver abscess (PLA).Recent publications from Central Europe and ...Knowledge of etiology and timely treatment of underlying causes,when possible,play an important role in the successful therapy of patients with pyogenic liver abscess (PLA).Recent publications from Central Europe and Southeast Asia hint at considerable differences in etiology.In this article,we aim to elaborate these differences and their therapeutic implications.Apart from some special types of PLA that are comparable in Southeast Asia and Central Europe (such as posttraumatic or postprocedural PLA),there are clear differences in the microbiological spectrum,which implies different risk factors and disease courses.Klebsiella pneumoniae (K.pneumoniae) PLA is predominantly seen in Southeast Asia,whereas,in Central Europe,PLA is typically caused by Escherichia coli,Streptococcus or Staphylococcus,and these patients are more likely to be older and to have a biliary abnormality or malignancy.K.pneumoniae patients are more likely to have diabetes mellitus.Control of septic spread is crucial in K.pneumoniae patients,whereas treatment of the underlying diseases is decisive in many Central European PLA patients.展开更多
Background The increased incidence of pyogenic liver abscess caused by Klebsiella pneumoniae （K.pneumoniae） was reported in the recent literature. This study was conducted retrospectively to investigate the clinical...Background The increased incidence of pyogenic liver abscess caused by Klebsiella pneumoniae （K.pneumoniae） was reported in the recent literature. This study was conducted retrospectively to investigate the clinical characteristics and outcomes of these patients. Methods Microbiological and medical databases of a medical center were searched from January 2000 to June 2003. Eighty-four patients with liver abscess caused by K. pneumoniae were analyzed. Results In the 84 patients, 52 men and 32 women aged （58.2±13.3） years on average, 64.4% had concomitant diabetes mellitus and 23.8% had biliary disease. The most common clinical symptoms were fever （98.8%）, chills （69.0%） and abdominal pain （58.3%）. 85.7% of the 84 patients received catheter drainage for the abscess. The length of hospital stay was （17.4 ±8.7） days. The mortality rate was 7.1%. Older age and presence of biliary disease were associated with mortality. Conclusions The low mortality of our patients was probably related to the high proportion of patients who received catheter drainage. Older age and presence of biliary disease were associated with the mortality.展开更多
The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing g...The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glu-cose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1—1.5g·L-1·h-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g·L-1·h-1.展开更多
This paper focuses on the research of the bioconversion of 1,3-propanediol by Klebsiella pneumoniae. The linear correlation of cell growth and 1,3-propanediol synthesis was found. An equation of the relationship betwe...This paper focuses on the research of the bioconversion of 1,3-propanediol by Klebsiella pneumoniae. The linear correlation of cell growth and 1,3-propanediol synthesis was found. An equation of the relationship between cell growth and biocatalysis was given.With the analysis of metabolism, it was discovered that the cell regulated the NADH production by cell growth in order to supply enough reductive equivalent for enzyme catalysis. A conclusion was drawn that the cell growth was coupled with the reactivation of a key-enzyme which catalyzes 1,3-propanediol production in Klebsiella pneumoniae.展开更多
基金This work was supported by the National Natural Science Foundation of China under Grant （No. 81201338）.
文摘Background： Klebsiellapneumoniae （KP） is a pathogen commonly causing nosocomial infection. Carbapenem-resistant KP （CRKP） is more resistant to multiple antimicrobial drugs than carbapenem-susceptible KP （CSKP） isolates. The aim of the present study was to identify the risk factors for CRKP infection and the predictors of mortality among KP-infected adult patients. Methods： Patients with CRKP and CSKP infection were categorized as the case group and control group, respectively, and we conducted a 1：1 ratio case-control study on these groups. The CRKP isolates collected were tested tbr antimicrobial susceptibility and presence of KP carbapenemase （KPC） gene. Clinical data were collected to identity risk factors for CRKP infection and mortality of KP infection. Risk factors were analyzed under univariable and multivariable logistic regression model. Results： The independent risk factors for CRKP infection were admission to Intensive Care Unit （odds ratio [OR]： 15.486, 95% confidence interval [CI]： 3.175-75.541, P 〈 0.001）; use of I3-1actams and [3-1actamase inhibitor combination （OR： 4.765, 95% CI： 1.508-15.055, P = 0.008）： use of cephalosporins （OR： 8.033, 95% CI： 1.623-39.763, P = P= 0.019）： and indwelling of urethral catheter （OR： 6.164, 95% CI： 1.847 0.011 ）; fluoroquinolones （OR： 6.090, 95% CI： 1.343-27.613, -20.578, P = 0.003）. However, older age （OR： 1.079, 95% CI： 1.005-1.158, P= 0.036）, Charlson comorbidity index （OR： 4.690, 95% CI：2.094-10.504, P= 0.000）, and aminoglycoside use （OR： 670.252, 95% CI： 6.577-68,307.730, P = 0.006） were identified as independent risk factors for patient deaths with KP infection. The mortality of CRKP group was higher than that of the CSKP group. KPC gene did not play a role in the CRKP group. CRKP mortality was high. Conclusion： Implementation of infection control measures and protection of the immunefunction are crucial.
文摘Knowledge of etiology and timely treatment of underlying causes,when possible,play an important role in the successful therapy of patients with pyogenic liver abscess (PLA).Recent publications from Central Europe and Southeast Asia hint at considerable differences in etiology.In this article,we aim to elaborate these differences and their therapeutic implications.Apart from some special types of PLA that are comparable in Southeast Asia and Central Europe (such as posttraumatic or postprocedural PLA),there are clear differences in the microbiological spectrum,which implies different risk factors and disease courses.Klebsiella pneumoniae (K.pneumoniae) PLA is predominantly seen in Southeast Asia,whereas,in Central Europe,PLA is typically caused by Escherichia coli,Streptococcus or Staphylococcus,and these patients are more likely to be older and to have a biliary abnormality or malignancy.K.pneumoniae patients are more likely to have diabetes mellitus.Control of septic spread is crucial in K.pneumoniae patients,whereas treatment of the underlying diseases is decisive in many Central European PLA patients.
文摘Background The increased incidence of pyogenic liver abscess caused by Klebsiella pneumoniae （K.pneumoniae） was reported in the recent literature. This study was conducted retrospectively to investigate the clinical characteristics and outcomes of these patients. Methods Microbiological and medical databases of a medical center were searched from January 2000 to June 2003. Eighty-four patients with liver abscess caused by K. pneumoniae were analyzed. Results In the 84 patients, 52 men and 32 women aged （58.2±13.3） years on average, 64.4% had concomitant diabetes mellitus and 23.8% had biliary disease. The most common clinical symptoms were fever （98.8%）, chills （69.0%） and abdominal pain （58.3%）. 85.7% of the 84 patients received catheter drainage for the abscess. The length of hospital stay was （17.4 ±8.7） days. The mortality rate was 7.1%. Older age and presence of biliary disease were associated with mortality. Conclusions The low mortality of our patients was probably related to the high proportion of patients who received catheter drainage. Older age and presence of biliary disease were associated with the mortality.
文摘The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glu-cose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1—1.5g·L-1·h-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g·L-1·h-1.
文摘This paper focuses on the research of the bioconversion of 1,3-propanediol by Klebsiella pneumoniae. The linear correlation of cell growth and 1,3-propanediol synthesis was found. An equation of the relationship between cell growth and biocatalysis was given.With the analysis of metabolism, it was discovered that the cell regulated the NADH production by cell growth in order to supply enough reductive equivalent for enzyme catalysis. A conclusion was drawn that the cell growth was coupled with the reactivation of a key-enzyme which catalyzes 1,3-propanediol production in Klebsiella pneumoniae.
