The purpose of this study is to evaluate the effects and the underline mechanisms of berberine on the cardiac function and left ventricular remodeling in rats with renovascular hypertension.The renovascular hypertensi...The purpose of this study is to evaluate the effects and the underline mechanisms of berberine on the cardiac function and left ventricular remodeling in rats with renovascular hypertension.The renovascular hypertensive model was established by the two-kidney,two-clip（2K2C） method in Sprague-Dawley（SD） rats.Two weeks after surgery,all the operated SD rats were randomly assigned into four groups: ① renovascular hypertensive model group;② berberine 5 mg·kg-1 group;③ berberine 10 mg·kg-1 group;④ captopril 45 mg·kg-1 group;and the sham operated rats were used as control.Four weeks after the drugs were administered,the cardiac function was assessed.The ratios of heart weight to body weight（HW/BW）,left ventricular weight to body weight（LVW/BW） and right ventricular weight to body weight（RVW/BW） were compared between groups.Coronal sections of the left ventricular tissue（LV） were prepared for paraffin sections,picrosirius red and HE staining was performed.The left ventricular wall thickness（LVWT）,interventricular septal thickness（IVST）,the parameters of myocardial fibrosis indicated by interstitial collagen volume fraction（ICVF） and perivascular collagen area（PVCA） were assessed.Nitric oxide（NO）,adenosine cyclophosphate（cAMP） and guanosine cyclophosphate（cGMP） concentrations of left ventricular tissue were measured.Berberine 5 mg·kg-1 and 10 mg·kg-1 increased the left ventricular ±dp/dtmax and HR.Berberine 10 mg·kg-1 decreased（HW/BW） and LVW/BW.The image analysis showed that both 5 and 10 mg·kg-1 of berberine decreased LVWT,ICVF and PVCA,while increased the NO and cAMP contents in left ventricular tissue.Berberine could improve cardiac contractility of 2K2C model rats,and inhibit left ventricular remodeling especially myocardial fibrosis in renovascular hypertension rats.And such effects may partially associate with the increased NO and cAMP content in left ventricular tissue.展开更多
Fe modified and un-modified K/Mo2C were prepared and investigated as catalysts for CO hydrogenation reaction. Compared with K/Mo2C catalyst, the addition of Fe increased the production of alcohols, especially the C2＋...Fe modified and un-modified K/Mo2C were prepared and investigated as catalysts for CO hydrogenation reaction. Compared with K/Mo2C catalyst, the addition of Fe increased the production of alcohols, especially the C2＋OH. Meanwhile, considerable amounts of C5＋ hydrocar- bons and C2= -C4= were formed, whereas methane selectivity greatly decreased. Also, the activity and selectivity of the catalyst were readily affected by the reaction pressure and temperature employed. According to the XPS results, Mo4＋ might be responsible for the production of alcohols, whereas the low valence state of Mo species such as Mo^0 and/or Mo^2＋ might be account for the high activity and selectivity toward hydrocarbons.展开更多
文摘The purpose of this study is to evaluate the effects and the underline mechanisms of berberine on the cardiac function and left ventricular remodeling in rats with renovascular hypertension.The renovascular hypertensive model was established by the two-kidney,two-clip（2K2C） method in Sprague-Dawley（SD） rats.Two weeks after surgery,all the operated SD rats were randomly assigned into four groups: ① renovascular hypertensive model group;② berberine 5 mg·kg-1 group;③ berberine 10 mg·kg-1 group;④ captopril 45 mg·kg-1 group;and the sham operated rats were used as control.Four weeks after the drugs were administered,the cardiac function was assessed.The ratios of heart weight to body weight（HW/BW）,left ventricular weight to body weight（LVW/BW） and right ventricular weight to body weight（RVW/BW） were compared between groups.Coronal sections of the left ventricular tissue（LV） were prepared for paraffin sections,picrosirius red and HE staining was performed.The left ventricular wall thickness（LVWT）,interventricular septal thickness（IVST）,the parameters of myocardial fibrosis indicated by interstitial collagen volume fraction（ICVF） and perivascular collagen area（PVCA） were assessed.Nitric oxide（NO）,adenosine cyclophosphate（cAMP） and guanosine cyclophosphate（cGMP） concentrations of left ventricular tissue were measured.Berberine 5 mg·kg-1 and 10 mg·kg-1 increased the left ventricular ±dp/dtmax and HR.Berberine 10 mg·kg-1 decreased（HW/BW） and LVW/BW.The image analysis showed that both 5 and 10 mg·kg-1 of berberine decreased LVWT,ICVF and PVCA,while increased the NO and cAMP contents in left ventricular tissue.Berberine could improve cardiac contractility of 2K2C model rats,and inhibit left ventricular remodeling especially myocardial fibrosis in renovascular hypertension rats.And such effects may partially associate with the increased NO and cAMP content in left ventricular tissue.
文摘采用真空浸渍法制备炭基固体碱催化剂K2O/C,并在超声波辅助条件下,利用制备的K2O/C催化纤维低聚糖与油酸甲酯制备纤维低聚糖脂肪酸酯表面活性剂。选取L9(34)正交试验确定制备K2O/C催化剂的最佳条件为:炭基载体平均孔径为2.87 nm,K2CO3与炭基载体的质量比值为0.5,真空浸渍后,450℃下煅烧2 h。考察了超声波辅助下超声波时间、超声波频率、反应温度、反应时间和催化剂用量对产品得率的影响。研究发现:在20 k Hz,150 W超声功率下,将物质的量之比为2∶1的油酸甲酯和纤维低聚糖(水溶液)超声波作用15 min,形成均一稳定的乳化体系,真空条件下,移除体系中的水分后,加入占总物料量5%的K2O/C催化剂,125℃下反应2 h,纤维低聚糖脂肪酸酯的最高得率为85.6%,其酯化度为18.8%,亲水亲油平衡值(HLB)为9.89,表面张力为32.1 m N/m,乳化力为28.1%,硬水稳定性4级。
基金supported by the Key Project of Chinese National Programs for Fundamental Research and Development(973 Program-2005CB221400)
文摘Fe modified and un-modified K/Mo2C were prepared and investigated as catalysts for CO hydrogenation reaction. Compared with K/Mo2C catalyst, the addition of Fe increased the production of alcohols, especially the C2＋OH. Meanwhile, considerable amounts of C5＋ hydrocar- bons and C2= -C4= were formed, whereas methane selectivity greatly decreased. Also, the activity and selectivity of the catalyst were readily affected by the reaction pressure and temperature employed. According to the XPS results, Mo4＋ might be responsible for the production of alcohols, whereas the low valence state of Mo species such as Mo^0 and/or Mo^2＋ might be account for the high activity and selectivity toward hydrocarbons.
