Antimicrobial resistance(AMR)is a pressing issue in China,with antibiotic therapy becoming less effective against bacterial infections.To address this challenge,the China Antimicrobial Surveillance Network(CHINET)was ...Antimicrobial resistance(AMR)is a pressing issue in China,with antibiotic therapy becoming less effective against bacterial infections.To address this challenge,the China Antimicrobial Surveillance Network(CHINET)was established in 2005 to monitor antimicrobial resistance in the country.This study analyzed the CHINET data from teaching hospi-tals and evaluated the trends of AMR in China from 2018 to 2022.A range of 163,636 to 301,917 isolates was obtained per year,with the majority being Gram-negative bacilli(69.0%to 71.8%).The proportion of important multidrug-resistant pathogens remained stable over the years.While the analysis showed diverse AMR profiles for different bac-terial species.Over the five years,generally decreased resistance rates were observed from the majority of the tested species.For example,resistance to ceftriaxone decreased in Escherichia coli and Klebsiella pneumoniae,while resistance to imipenem and meropenem decreased in Pseudomonas aeruginosa.Moreover,resistance to methicillin,gentamicin,fosfomycin,and clindamycin also decreased in clinical Staphylococcus aureus isolates.On the other hand,resistance levels of Acinetobacter baumannii remained stable.Our study provides a comprehensive overview of the AMR profiles of common bacterial species in China and highlights the ongoing efforts to address this challenge.展开更多
The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to...The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to inves-tigate the epidemic features and transmission dynamics of tet(X4)-positive Klebsiella pneumoniae(K.pneumo-niae)through the analysis of 206 raw meats,including pork(n=182),beef(n=16),duck(n=5),and chicken(n=3).These samples were collected from schools,markets,and restaurants in Chengdu City,China.A total of 25 isolates were obtained from 13 administrative regions.All isolates exhibited resistance to tetracycline,tigecycline,ampicillin,chloramphenicol,and florfenicol.Over half of the isolates also demon-strated resistance to streptomycin(80%),sulfamethoxazole/trimethoprim(72%),ciprofloxacin(64%),and ampicillin/sulbactam(56%).Among these strains,14 distinct sequence types(STs)were identified,revealing evidence of inter-regional clonal spread,notably among 9 K.pneumoniae ST3393.Phylogenetic analysis revealed the presence of two K.pneumoniae ST5 closely resembling hypervirulent K.pneumoniae from Jiangsu.Importantly,12 isolates were capable of transferring tigecycline resistance to Escherichia coli J53.Further plasmid analysis showed that the tet(X4)-harboring plasmids in K.pneumoniae could be classified into four types,primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid(n=16)and IncFII plasmid(n=7),which significantly contributed to the cross-species dissemination of tet(X4).In summary,this study highlights the prevalence of MDR tet(X4)-positive K.pneumoniae in Chengdu,driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer.These findings emphasize the importance of contin-uous surveillance of tet(X4)-positive K.pneumoniae in raw meat and the implementation of effective measures to control their spread.展开更多
基金funded by the National Key Research and Development Program of China(2021YFC2701800 and 2021YFC2701803)the National Natural Science Foundation of China(grant no.81861138052)+1 种基金the China Antimicrobial Surveillance Network(independent medical grants from Pfizer,2018QD100)the Shanghai Antimicrobial Surveillance Network(3030231003).
文摘Antimicrobial resistance(AMR)is a pressing issue in China,with antibiotic therapy becoming less effective against bacterial infections.To address this challenge,the China Antimicrobial Surveillance Network(CHINET)was established in 2005 to monitor antimicrobial resistance in the country.This study analyzed the CHINET data from teaching hospi-tals and evaluated the trends of AMR in China from 2018 to 2022.A range of 163,636 to 301,917 isolates was obtained per year,with the majority being Gram-negative bacilli(69.0%to 71.8%).The proportion of important multidrug-resistant pathogens remained stable over the years.While the analysis showed diverse AMR profiles for different bac-terial species.Over the five years,generally decreased resistance rates were observed from the majority of the tested species.For example,resistance to ceftriaxone decreased in Escherichia coli and Klebsiella pneumoniae,while resistance to imipenem and meropenem decreased in Pseudomonas aeruginosa.Moreover,resistance to methicillin,gentamicin,fosfomycin,and clindamycin also decreased in clinical Staphylococcus aureus isolates.On the other hand,resistance levels of Acinetobacter baumannii remained stable.Our study provides a comprehensive overview of the AMR profiles of common bacterial species in China and highlights the ongoing efforts to address this challenge.
基金supported by the National Key Research and Development Program of China(No.2022YFD1800400)National Natural Science Foundation of China(No.81991531)+1 种基金Natural Science Foundation of Shandong Province(No.ZR2020MC188)The sequence data of 25 K.pneumoniae isolates have been submitted to NCBI under BioProject accession number PRJNA1011004.
文摘The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to inves-tigate the epidemic features and transmission dynamics of tet(X4)-positive Klebsiella pneumoniae(K.pneumo-niae)through the analysis of 206 raw meats,including pork(n=182),beef(n=16),duck(n=5),and chicken(n=3).These samples were collected from schools,markets,and restaurants in Chengdu City,China.A total of 25 isolates were obtained from 13 administrative regions.All isolates exhibited resistance to tetracycline,tigecycline,ampicillin,chloramphenicol,and florfenicol.Over half of the isolates also demon-strated resistance to streptomycin(80%),sulfamethoxazole/trimethoprim(72%),ciprofloxacin(64%),and ampicillin/sulbactam(56%).Among these strains,14 distinct sequence types(STs)were identified,revealing evidence of inter-regional clonal spread,notably among 9 K.pneumoniae ST3393.Phylogenetic analysis revealed the presence of two K.pneumoniae ST5 closely resembling hypervirulent K.pneumoniae from Jiangsu.Importantly,12 isolates were capable of transferring tigecycline resistance to Escherichia coli J53.Further plasmid analysis showed that the tet(X4)-harboring plasmids in K.pneumoniae could be classified into four types,primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid(n=16)and IncFII plasmid(n=7),which significantly contributed to the cross-species dissemination of tet(X4).In summary,this study highlights the prevalence of MDR tet(X4)-positive K.pneumoniae in Chengdu,driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer.These findings emphasize the importance of contin-uous surveillance of tet(X4)-positive K.pneumoniae in raw meat and the implementation of effective measures to control their spread.
