MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家...MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家族基因在骨骼肌中的表达变化,并分析其表达变化与胚重、骨骼肌发育的相关性。金定鸭胚重及骨骼肌重发育模式显示胚重在胚胎期呈持续增加趋势,但胸肌在胚胎中后期显示出较腿肌滞后的增重变化。MRFs家族基因在胚胎期骨骼肌中均能检测到表达,Myo D m RNA在21日胚龄前腿肌中的表达量高于胸肌,而在胚胎发育后期(25及27日胚龄)胸肌中表达量渐渐高于腿肌。胸、腿肌中myogenin m RNA的表达与胚重、胸、腿肌发育重量变化均呈强的负线性相关。上述研究结果首次揭示了鸭胚胎期骨骼肌中MRFs家族基因表达具有显著的时空性,推测Myo D m RNA在胸、腿肌中的表达变化可能与胸、腿肌增重表型差异有关,研究结果为进一步深入研究MRFs家族基因在胚胎期骨骼肌发生过程及其调控机理中的功能提供一定的理论依据。展开更多
[Objeclive] The comparison of levels of thyroid hormones （T3, T4 and FT3） in female ducks serum and embryo of different embryonic age between Gaoyou duck and Jinding duck were made to provide basic data for further ...[Objeclive] The comparison of levels of thyroid hormones （T3, T4 and FT3） in female ducks serum and embryo of different embryonic age between Gaoyou duck and Jinding duck were made to provide basic data for further research. [Method] The levels of thyroid hormones （T3, T4 and FT3） in female ducks serum, fertilized eggs, and 7, 11,15, 19, 22, 28-days embryo of Gaoyou duck and Jinding duck were analyzed with radioimmunoassay （RIA）. [ Result]The levels of thyroid hormones in fertilized eggs were higher than those in serum of female ducks; different em- bryonic age hormone level of Gaoyou duck and Jinding duck in serum had almost the same variation trend, which was low but relatively stable after 15 days. [Condusion] The result suggested that thyroid hormones play an important role in duck embryonic development, especially in the early peded.展开更多
文摘MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家族基因在骨骼肌中的表达变化,并分析其表达变化与胚重、骨骼肌发育的相关性。金定鸭胚重及骨骼肌重发育模式显示胚重在胚胎期呈持续增加趋势,但胸肌在胚胎中后期显示出较腿肌滞后的增重变化。MRFs家族基因在胚胎期骨骼肌中均能检测到表达,Myo D m RNA在21日胚龄前腿肌中的表达量高于胸肌,而在胚胎发育后期(25及27日胚龄)胸肌中表达量渐渐高于腿肌。胸、腿肌中myogenin m RNA的表达与胚重、胸、腿肌发育重量变化均呈强的负线性相关。上述研究结果首次揭示了鸭胚胎期骨骼肌中MRFs家族基因表达具有显著的时空性,推测Myo D m RNA在胸、腿肌中的表达变化可能与胸、腿肌增重表型差异有关,研究结果为进一步深入研究MRFs家族基因在胚胎期骨骼肌发生过程及其调控机理中的功能提供一定的理论依据。
基金funded by State Funds($31172194)Jiangsu Province Modern Agricultural Variety Innovation CX(11)(1030)+1 种基金Science and Technology Support Plan(BE2011329)Yangzhou City Science and Technology Research(YZ2010048)
文摘[Objeclive] The comparison of levels of thyroid hormones （T3, T4 and FT3） in female ducks serum and embryo of different embryonic age between Gaoyou duck and Jinding duck were made to provide basic data for further research. [Method] The levels of thyroid hormones （T3, T4 and FT3） in female ducks serum, fertilized eggs, and 7, 11,15, 19, 22, 28-days embryo of Gaoyou duck and Jinding duck were analyzed with radioimmunoassay （RIA）. [ Result]The levels of thyroid hormones in fertilized eggs were higher than those in serum of female ducks; different em- bryonic age hormone level of Gaoyou duck and Jinding duck in serum had almost the same variation trend, which was low but relatively stable after 15 days. [Condusion] The result suggested that thyroid hormones play an important role in duck embryonic development, especially in the early peded.
