Twenty-one lignans including three new ones(1, 2 and 13) were isolated from Justicia procumbens. The chemical structures of the new lignans were determined by spectroscopic means including 1 D and 2 D NMR analysis. Th...Twenty-one lignans including three new ones(1, 2 and 13) were isolated from Justicia procumbens. The chemical structures of the new lignans were determined by spectroscopic means including 1 D and 2 D NMR analysis. These compounds were evaluated for their cytotoxic and anti-HIV activities. The new secoisolariciresinol dimethyl ether acetate(13) exhibited anti-HIV-1 activity with an IC50 value of 5.27 μmol·L^-1 and a selective index(SI) value of 2.2. The known arylnaphthalene lignan procumbenoside A(3) and diphyllin(8) demonstrated inhibitory activity against HIV-1 with IC50 values of 4.95(SI > 6.2) and 0.38 μmol·L^-1(SI = 5.3), respectively.展开更多
The use of plant parts (leaves, flowers, stems, barks, roots etc.) in traditional medicine is increasingly gaining ground in modern medicine, as plant sources have long been recognized as sources of secondary metaboli...The use of plant parts (leaves, flowers, stems, barks, roots etc.) in traditional medicine is increasingly gaining ground in modern medicine, as plant sources have long been recognized as sources of secondary metabolites which can be used to treat a wide range of diseases. The effect of extracts of Justicia secunda leaves on red blood cells (RBC) count and haemoglobin (Hb) concentration was investigated in adult Sprague-Dawley rats to establish haematinic activity. Phenylhydrazine (PHZ)-induced anaemic rats were treated with water, methanol or ethyl acetate extracts at 200 mg/kg body weight. RBC counts and Hb concentration were analysed using a haematology analyser at 3-day intervals for 21 days. The extracts were compared with rats administered the haematinic tonic Feroglobin®?and vehicle-treated (normal saline). Rats administered the water extract exhibited the most significant increase (P ) in the number of RBCs and Hb concentration compared with the vehicle-treated PHZ-induced anaemic rats. Rats administered the methanol extract followed with significant increase (P ) in RBC counts and Hb concentration (J. secunda leaves has excellent haematinic properties and this provides the pharmacological basis of its use in Ghanaian traditional medicine for the treatment of anaemia.展开更多
Objective: To synthesize bio-inspired gold nanoparticles(AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line(A549).Methods: Synthesis of AuNPs was do...Objective: To synthesize bio-inspired gold nanoparticles(AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line(A549).Methods: Synthesis of AuNPs was done using an aqueous leaf extract of Justicia adhatoda as a green route. The bio-synthesized AuNPs were confirmed and characterized by using various spectral studies such as UV-Vis spectrum, Scanning Electron Microscope with EDAX, Transmission Electron Microscope, Fourier Transmission Infrared Spectroscope analysis and Surface Enhanced Raman Spectroscopy. The cell viability was determined by MTT reduction assay. In addition, cytomorphology and the nuclear morphological study of A549 cell line was observed under fluorescence microscope. Results: UV-Vis spectrum showed surface plasmon resonance peak at 547 nm, scanning electron microscope and transmission electron microscope studies showed the monodispersed spherical shape and its average size in the range of 40.1 nm was noticed. Fourier Transmission Infrared Spectroscope analysis confirmed that the C=O group of amino acids of proteins had strong ability to bind with the surface of nanoparticle. Interestingly, our results also demonstrated inhibited proliferation of A549 cell line by MTT(IC50 value: 80 μg/mL). Cell morphology was observed and cell death was caused by apoptosis as revealed by propidium iodide staining. Conclusions: The current study proves the anticancer potential of bio-synthesized AuNPs. Thus, synthesized AuNPs can be used for the treatment of human lung cancer cell(A549) and it can be exploited for drug delivery in future.展开更多
A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in ...A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in Justicia and then moved to Mananthes.The new genus is characterized by its shrub habit,strongly 2-lipped corolla,the 2-lobed upper lip,3-lobed lower lip,2 stamens,bithecous anthers,parallel thecae with two spurs at the base,2 ovules in each locule,and the 4-seeded capsule.Phylogenetic analyses show that the new genus belongs to the Pseuderanthemum lineage in tribe Justicieae.Wuacanthus is closely related to Pseuderanthemum but differs from the latter by its shorter corolla tube and two minute spurs at the base of each anther-theca.W.microdontus is assessed with the status EN B2ab(iii)based on the IUCN Red List Categories and Criteria.展开更多
Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This ...Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antihyperlipidemic agent.Methods: Antihyperlipidemic effect was assessed in a high-fat diet-induced hyperlipidemic model in Wistar albino rats. The rats were treated orally with extracts of bashok(J adhatoda, 200 mg/kg bw), tulshi(O tenuiflorum, 200 mg/kg bw), and a combination of bashok and tulshi(50:50), as well as with a reference drug, atorvastatin(10 mg/kg/day), with or without high-fat diet for 14 days. The antioxidative effect was studied using established in vitro models. The studies were supported by experimentally testing the effects of the extracts on membrane stabilization and inhibition of protein denaturation.Results: The results showed that the serum lipid profile was significantly decreased in the different treatment groups, with bashok having the greatest effect. Body weights, total serum protein, LDH, and relative liver and adipose tissue weights were markedly restored towards baseline values, the lowest atherogenic index being achieved with the combined extract. The combination treatment significantly enhanced total phenolic content and antioxidative capacity and greatly potentiated membrane stabilization, but inhibition of protein denaturation was not significantly affected.Conclusion: The data demonstrate that a combination of Justicia adhatoda and Ocimum tenuiflorum could be developed as a food supplement with antioxidative and antihyperlipidemic benefits.展开更多
The Acanthaceae family is an important source of therapeutic drugs and the ethno pharmacological knowledge of this family requires urgent documentation as several of its species are near extinction.Justicia is the lar...The Acanthaceae family is an important source of therapeutic drugs and the ethno pharmacological knowledge of this family requires urgent documentation as several of its species are near extinction.Justicia is the largest genus of Acanthaceae with approximately 600 species.Aim of the present review is to present literature for the traditional uses&pharmacological review of Justicia picta(Family:Acanthaceae)and to discuss further priorities of research yet to be discovered.展开更多
Objective:To investigate traditional uses of medicinal plant Justicia adhatoda L.by Meitei community in Manipur.Methods:Surveys were carried out in four districts in Central Valley of Manipur viz.,Imphal East,Imphal W...Objective:To investigate traditional uses of medicinal plant Justicia adhatoda L.by Meitei community in Manipur.Methods:Surveys were carried out in four districts in Central Valley of Manipur viz.,Imphal East,Imphal West,Bishnupur and Thoubal between 2008-2013 to collect information on the ethnobotanical uses of the species.Local people and Maiba/maibis(men/women healers)in the study areas were interviewed and preparation techniques of medicine and cuisines from the plant parts were demonstrated and photographed.Results:It was found that Meitei community in the study area extensively used Justicia adhatoda L.as ethno-medicine as well as food.Young and mature green leaves were used as traditional medicine in different ways to cure many ailments like cough,fever,asthma and dysentery.The ethnic community used inflorescences and leaves of the plant to prepare different cuisines for good digestion and health.Conclusions:Documentation of detailed ethnobotanical uses of individual plant species is need of the hour and it will help in understanding the in-depth knowledge about the role of species in the society.展开更多
小驳骨为爵床科药用植物,具有祛瘀止痛、续筋接骨的功效,长期以来被用于跌打损伤、筋伤骨折、风湿骨痛、血瘀经闭和产后腹痛等疾病的治疗。通过搜索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的小驳骨相关文献,对小...小驳骨为爵床科药用植物,具有祛瘀止痛、续筋接骨的功效,长期以来被用于跌打损伤、筋伤骨折、风湿骨痛、血瘀经闭和产后腹痛等疾病的治疗。通过搜索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的小驳骨相关文献,对小驳骨近二十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从小驳骨中分离出超过100种化合物,包括萜类、黄酮类、苯丙素类、木质素类、香豆素类和生物碱等。小驳骨提取物具有显著的生物活性,如抗炎、抗氧化等,其抗炎作用机制可能与调节炎症因子水平、抑制相关信号通路或相关蛋白表达等有关。可为小驳骨资源后续深入系统地研究和临床开发提供科学依据和理论参考。展开更多
基金supported by the Research Grants Council of the Hong Kong Special Administrative Region,China(Nos.HKBU12103618 and HKBU12103917)the Research Grants Council of the Hong Kong Baptist University(HKBU)+2 种基金Interdisciplinary Research Matching Scheme(No.RC-IRMS/15-16/02)the Hong Kong Scholars Program Foundation(No.XJ2015047)Mr.Kwok Yat Wai and Madam Kwok Chung Bo Fun Graduate School Development Fund(WANG Dong-Ying)
文摘Twenty-one lignans including three new ones(1, 2 and 13) were isolated from Justicia procumbens. The chemical structures of the new lignans were determined by spectroscopic means including 1 D and 2 D NMR analysis. These compounds were evaluated for their cytotoxic and anti-HIV activities. The new secoisolariciresinol dimethyl ether acetate(13) exhibited anti-HIV-1 activity with an IC50 value of 5.27 μmol·L^-1 and a selective index(SI) value of 2.2. The known arylnaphthalene lignan procumbenoside A(3) and diphyllin(8) demonstrated inhibitory activity against HIV-1 with IC50 values of 4.95(SI > 6.2) and 0.38 μmol·L^-1(SI = 5.3), respectively.
文摘The use of plant parts (leaves, flowers, stems, barks, roots etc.) in traditional medicine is increasingly gaining ground in modern medicine, as plant sources have long been recognized as sources of secondary metabolites which can be used to treat a wide range of diseases. The effect of extracts of Justicia secunda leaves on red blood cells (RBC) count and haemoglobin (Hb) concentration was investigated in adult Sprague-Dawley rats to establish haematinic activity. Phenylhydrazine (PHZ)-induced anaemic rats were treated with water, methanol or ethyl acetate extracts at 200 mg/kg body weight. RBC counts and Hb concentration were analysed using a haematology analyser at 3-day intervals for 21 days. The extracts were compared with rats administered the haematinic tonic Feroglobin®?and vehicle-treated (normal saline). Rats administered the water extract exhibited the most significant increase (P ) in the number of RBCs and Hb concentration compared with the vehicle-treated PHZ-induced anaemic rats. Rats administered the methanol extract followed with significant increase (P ) in RBC counts and Hb concentration (J. secunda leaves has excellent haematinic properties and this provides the pharmacological basis of its use in Ghanaian traditional medicine for the treatment of anaemia.
文摘Objective: To synthesize bio-inspired gold nanoparticles(AuNPs) using the leaf extract of Justicia adhatoda and evaluate the anti-cancer activity on human lung cancer cell line(A549).Methods: Synthesis of AuNPs was done using an aqueous leaf extract of Justicia adhatoda as a green route. The bio-synthesized AuNPs were confirmed and characterized by using various spectral studies such as UV-Vis spectrum, Scanning Electron Microscope with EDAX, Transmission Electron Microscope, Fourier Transmission Infrared Spectroscope analysis and Surface Enhanced Raman Spectroscopy. The cell viability was determined by MTT reduction assay. In addition, cytomorphology and the nuclear morphological study of A549 cell line was observed under fluorescence microscope. Results: UV-Vis spectrum showed surface plasmon resonance peak at 547 nm, scanning electron microscope and transmission electron microscope studies showed the monodispersed spherical shape and its average size in the range of 40.1 nm was noticed. Fourier Transmission Infrared Spectroscope analysis confirmed that the C=O group of amino acids of proteins had strong ability to bind with the surface of nanoparticle. Interestingly, our results also demonstrated inhibited proliferation of A549 cell line by MTT(IC50 value: 80 μg/mL). Cell morphology was observed and cell death was caused by apoptosis as revealed by propidium iodide staining. Conclusions: The current study proves the anticancer potential of bio-synthesized AuNPs. Thus, synthesized AuNPs can be used for the treatment of human lung cancer cell(A549) and it can be exploited for drug delivery in future.
基金supported by the National Natural Science Foundation of China (grant nos. 30670124,31270247,31470302,31400184,31670191,31110103911)Science and Technology Basic Work (grant no. 2013FY112100)
文摘A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in Justicia and then moved to Mananthes.The new genus is characterized by its shrub habit,strongly 2-lipped corolla,the 2-lobed upper lip,3-lobed lower lip,2 stamens,bithecous anthers,parallel thecae with two spurs at the base,2 ovules in each locule,and the 4-seeded capsule.Phylogenetic analyses show that the new genus belongs to the Pseuderanthemum lineage in tribe Justicieae.Wuacanthus is closely related to Pseuderanthemum but differs from the latter by its shorter corolla tube and two minute spurs at the base of each anther-theca.W.microdontus is assessed with the status EN B2ab(iii)based on the IUCN Red List Categories and Criteria.
基金Supported by the National Natural Science Foundation of China (no. 30370109), from the Special Project for Taxonomic andFloristic Researches from the Chinese Academy of Sciences (to Xia) and the Director Foundation of South China Botanical Garden, CAS (to Deng).
基金National Natural Science Foundation of China (30370109,30670142,30870152,39899400,30499340)Pilot Project of Knowledge Innovation,the Chinese Academy of Sciences (KSCX-SW-122,KSCX2-YW-Z-021)Lakeside Foundation through the California Academy of Sciences
基金funded by Chittagong University Research Cell,grant number#curespub/2016/13。
文摘Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antihyperlipidemic agent.Methods: Antihyperlipidemic effect was assessed in a high-fat diet-induced hyperlipidemic model in Wistar albino rats. The rats were treated orally with extracts of bashok(J adhatoda, 200 mg/kg bw), tulshi(O tenuiflorum, 200 mg/kg bw), and a combination of bashok and tulshi(50:50), as well as with a reference drug, atorvastatin(10 mg/kg/day), with or without high-fat diet for 14 days. The antioxidative effect was studied using established in vitro models. The studies were supported by experimentally testing the effects of the extracts on membrane stabilization and inhibition of protein denaturation.Results: The results showed that the serum lipid profile was significantly decreased in the different treatment groups, with bashok having the greatest effect. Body weights, total serum protein, LDH, and relative liver and adipose tissue weights were markedly restored towards baseline values, the lowest atherogenic index being achieved with the combined extract. The combination treatment significantly enhanced total phenolic content and antioxidative capacity and greatly potentiated membrane stabilization, but inhibition of protein denaturation was not significantly affected.Conclusion: The data demonstrate that a combination of Justicia adhatoda and Ocimum tenuiflorum could be developed as a food supplement with antioxidative and antihyperlipidemic benefits.
基金Supported by Teerthanker Mahaveer University,Delhi Road,Bagarpur,Moradabad,Uttar Pradesh,India(Grant No.TMU/TMCOP/2013-14/RES.GRANT-01/PGN).
文摘The Acanthaceae family is an important source of therapeutic drugs and the ethno pharmacological knowledge of this family requires urgent documentation as several of its species are near extinction.Justicia is the largest genus of Acanthaceae with approximately 600 species.Aim of the present review is to present literature for the traditional uses&pharmacological review of Justicia picta(Family:Acanthaceae)and to discuss further priorities of research yet to be discovered.
基金Supported by North East Centre for Environmental Education and Research,Imphal(Manipur,India)(Grant No.22/EMP/M/2008-RD).
文摘Objective:To investigate traditional uses of medicinal plant Justicia adhatoda L.by Meitei community in Manipur.Methods:Surveys were carried out in four districts in Central Valley of Manipur viz.,Imphal East,Imphal West,Bishnupur and Thoubal between 2008-2013 to collect information on the ethnobotanical uses of the species.Local people and Maiba/maibis(men/women healers)in the study areas were interviewed and preparation techniques of medicine and cuisines from the plant parts were demonstrated and photographed.Results:It was found that Meitei community in the study area extensively used Justicia adhatoda L.as ethno-medicine as well as food.Young and mature green leaves were used as traditional medicine in different ways to cure many ailments like cough,fever,asthma and dysentery.The ethnic community used inflorescences and leaves of the plant to prepare different cuisines for good digestion and health.Conclusions:Documentation of detailed ethnobotanical uses of individual plant species is need of the hour and it will help in understanding the in-depth knowledge about the role of species in the society.
文摘小驳骨为爵床科药用植物,具有祛瘀止痛、续筋接骨的功效,长期以来被用于跌打损伤、筋伤骨折、风湿骨痛、血瘀经闭和产后腹痛等疾病的治疗。通过搜索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的小驳骨相关文献,对小驳骨近二十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从小驳骨中分离出超过100种化合物,包括萜类、黄酮类、苯丙素类、木质素类、香豆素类和生物碱等。小驳骨提取物具有显著的生物活性,如抗炎、抗氧化等,其抗炎作用机制可能与调节炎症因子水平、抑制相关信号通路或相关蛋白表达等有关。可为小驳骨资源后续深入系统地研究和临床开发提供科学依据和理论参考。
基金Supported by the National Natural Science Foundation of China(30670142,39899400,30499340)the Pilot Project of Knowledge Innovation Chinese Academy of Sciences(KSCX-SW-122)
