There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new me...There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new member of MMPs, was sequenced and identified recently. Expression of MMP-28 mRNA and protein in the cytotrophoblast cells and a choriocarcinoma cell line, JEG-3 cell, was conducted by zymography, RT-PCR and Northern blot. There is MMP-28 mRNA expression in both the cytotrophoblast cells and JEG-3 cells by RT-PCR. The activity of MMP-28 in cytotrophoblast cells was significantly weaker than that in JEG-3 (P 【 0.01) by zymography. Furthermore, mRNA expression of MMP-28 was significantly stronger (P 【 0.001) in JEG-3 than in human cytotrophoblast cells in a time-dependent way by Northern Blot. Our results suggest that MMP-28 may play a role in some of the tissue-remodeling events associated with normal pregnancy and tumor progression.展开更多
Objective:To study the effect of crude methanol and n-hexane extracts of Hypericum connatum(H.connatum) and Hypericum caprifoliatum on trophoblast-like cells.Methods:BeWo and JEG-3 trophoblast-like cells were submitte...Objective:To study the effect of crude methanol and n-hexane extracts of Hypericum connatum(H.connatum) and Hypericum caprifoliatum on trophoblast-like cells.Methods:BeWo and JEG-3 trophoblast-like cells were submitted to different extract concentrations(1.5,10 and 15 μg/mL) and evaluated in relation to cell viability and in titro trophoblast differentiation and function.Cell viability was evaluated using WST-1 reagent.Differentiation was measured by luciferase production,hCG production/release,and mitogenactivated protein kinase signaling pathway activation.The function of the trophoblast-like cells was measured by^(25)Ca^(2*) influx evaluation.Results:The results showed a decrease in cell viability/proliferation.Both plants and different extracts induced a significant decrease in hCG production/release and luciferase production.H.connatum did not cause mitogen-activated protein kinase signaling pathway disturbance;however,Hypericum caprifoliatum n-hexane extract at 15 μg/mL inhibited extracellular signalregulated kinase 1/2 activation.The significant increase in Ca^(2+) influx by JEG-3 cells was seen after short and long incubation times with H.connatum methanolie extract at 15 μg/mL.Conclusions:The results indicated that these two Hypericum species extracts can interfere on trophoblast differentiation and Ca^(2+)influx,according to their molecular diversity.Although in vivo experiments are necessary to establish their action on placental formation and function,this study suggests that attention must be paid to the potential toxic effect of these plants.展开更多
基金This work was supported by the Special Funds for the Major State Basic Research Project (Grant No. G1999055903)the Climbing Project of China (Grant No. 970211019-3)the Hundred-Scientist-Program of the Chinese Academy of Sciences.
文摘There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new member of MMPs, was sequenced and identified recently. Expression of MMP-28 mRNA and protein in the cytotrophoblast cells and a choriocarcinoma cell line, JEG-3 cell, was conducted by zymography, RT-PCR and Northern blot. There is MMP-28 mRNA expression in both the cytotrophoblast cells and JEG-3 cells by RT-PCR. The activity of MMP-28 in cytotrophoblast cells was significantly weaker than that in JEG-3 (P 【 0.01) by zymography. Furthermore, mRNA expression of MMP-28 was significantly stronger (P 【 0.001) in JEG-3 than in human cytotrophoblast cells in a time-dependent way by Northern Blot. Our results suggest that MMP-28 may play a role in some of the tissue-remodeling events associated with normal pregnancy and tumor progression.
基金Supported by Canadian Institutes of Health Research(CIHRNET 54002)
文摘Objective:To study the effect of crude methanol and n-hexane extracts of Hypericum connatum(H.connatum) and Hypericum caprifoliatum on trophoblast-like cells.Methods:BeWo and JEG-3 trophoblast-like cells were submitted to different extract concentrations(1.5,10 and 15 μg/mL) and evaluated in relation to cell viability and in titro trophoblast differentiation and function.Cell viability was evaluated using WST-1 reagent.Differentiation was measured by luciferase production,hCG production/release,and mitogenactivated protein kinase signaling pathway activation.The function of the trophoblast-like cells was measured by^(25)Ca^(2*) influx evaluation.Results:The results showed a decrease in cell viability/proliferation.Both plants and different extracts induced a significant decrease in hCG production/release and luciferase production.H.connatum did not cause mitogen-activated protein kinase signaling pathway disturbance;however,Hypericum caprifoliatum n-hexane extract at 15 μg/mL inhibited extracellular signalregulated kinase 1/2 activation.The significant increase in Ca^(2+) influx by JEG-3 cells was seen after short and long incubation times with H.connatum methanolie extract at 15 μg/mL.Conclusions:The results indicated that these two Hypericum species extracts can interfere on trophoblast differentiation and Ca^(2+)influx,according to their molecular diversity.Although in vivo experiments are necessary to establish their action on placental formation and function,this study suggests that attention must be paid to the potential toxic effect of these plants.
