It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in tumor invasion and metastasis. The endoplasmic reticulum (ER)- retained, single chain Fv antibody ...It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in tumor invasion and metastasis. The endoplasmic reticulum (ER)- retained, single chain Fv antibody fragment (scFv) was used to inhibit the function of type IV collagenase. For expression in mammalian cells, the assembled scFv M97 gene with ER retention signal encoding 6 additional amino acids (SEKDEL) was reamplified by PCR. The amplified fragments were cloned into the pcDNA3.1 vector. The resulting plasmid was sequenced and then introduced into PG cells, a highly metastatic human lung cancer cell line, by lipofectAMINE method. The result of intrabody gene therapy showed that type IV collegenase expression was down regulated significantly as measured by ELISA. The biological behavior of PG cell, such as the ability of in vitro invasion through Matrigel, colony formation on soft agar, was also inhibited by scFv M97 transfection. Animal experiments in a xenograft model of human lung cancer showed that scFv M97 transfection significantly prolonged the survival time of nude mice. The results indicate that intracellular antibody technology represents a novel and efficient way to abrogate selectively the activity of type IV collagenase.展开更多
The galvanic-coupled intrabody communication(GCIBC)uses the human body as the channel for data transmission.The signal flow through different paths within different measure-ment schemes,resulting in distinct deviation...The galvanic-coupled intrabody communication(GCIBC)uses the human body as the channel for data transmission.The signal flow through different paths within different measure-ment schemes,resulting in distinct deviations in the experimental results.This method completely uses human body channel to transmit low-power signals,and has good anti-interference perform-ance.However,for a long time,the channel research of GCIBC has not determined a set of recog-nized and accurate measurement methods and standards.However,few researcher analyze the dif-ferences and principles between different measurement methods.This paper aims at three com-monly used measurement methods.Abstract the equivalent schematic diagrams of three measure-ment schemes:direct measurement,using high-precision differential probe and using balun.The sig-nal return paths generated by three measurement methods are analyzed,the impedance matching and energy transmission efficiency are compared.According to the analysis,the empirical expres-sions in different cases are listed.The mechanism between the channel measurement results gener-ated by the set experimental configuration and the comparison experiment is clarified.The compar-ison experiment with complete floating measurement is carried out as a reference to verify the pro-posed opinion.The measurement error caused by different experimental configurations is verified.This study can provide a reference opinions for the experimental error analysis of GCIBC research in the future,and provide suggestions for the selection of experimental configuration.展开更多
Intrabody communication(IBC)technology is becoming progressively more standard-ized due to its low power consumption and high security features incorporated into the third phys-ical layer of the IEEE 802.15.6 standard...Intrabody communication(IBC)technology is becoming progressively more standard-ized due to its low power consumption and high security features incorporated into the third phys-ical layer of the IEEE 802.15.6 standard.Even then,there are still many challenges in normalizing the measurement issues of IBC.A major concern that should not be overlooked is the electrodes in the IBC,especially the popular use of gel electrodes.In the channel measurements,gel electrodes are commonly employed to improve the signal-to-noise ratio and prevent electrodes from falling off.In this paper,a comparative study of the electrical properties of gel was investigated during the measurement of human channel characteristics and to clarify the differences of them.Firstly,the basis of electrostatic field pole plate measurements and electromagnetic theory were introduced to interpretate how the relative permittivity and conductivity of different gels will influence the meas-urement results.Then the in vivo experiments with different gel or dry electrodes were performed to compare the differences induced by the gel.The results indicate that the influence of the gel on the human channel measurement is mainly concentrated below 400 kHz(the attenuation is re-duced by 16.7 dB on average),and the stability of the permittivity and conductivity of the gel has a direct impact on the stability of its measurement of the human channel.This result may provide a meaningful reference for the standardization of electrode usage in IBC.展开更多
文摘It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in tumor invasion and metastasis. The endoplasmic reticulum (ER)- retained, single chain Fv antibody fragment (scFv) was used to inhibit the function of type IV collagenase. For expression in mammalian cells, the assembled scFv M97 gene with ER retention signal encoding 6 additional amino acids (SEKDEL) was reamplified by PCR. The amplified fragments were cloned into the pcDNA3.1 vector. The resulting plasmid was sequenced and then introduced into PG cells, a highly metastatic human lung cancer cell line, by lipofectAMINE method. The result of intrabody gene therapy showed that type IV collegenase expression was down regulated significantly as measured by ELISA. The biological behavior of PG cell, such as the ability of in vitro invasion through Matrigel, colony formation on soft agar, was also inhibited by scFv M97 transfection. Animal experiments in a xenograft model of human lung cancer showed that scFv M97 transfection significantly prolonged the survival time of nude mice. The results indicate that intracellular antibody technology represents a novel and efficient way to abrogate selectively the activity of type IV collagenase.
基金the National Natural Science Found-ation of China(No.U1505251)the International Coopera-tion Project in Fujian Province(No.2021I0005)Project of Education Department of Fujian Province(No.JAT200051).
文摘The galvanic-coupled intrabody communication(GCIBC)uses the human body as the channel for data transmission.The signal flow through different paths within different measure-ment schemes,resulting in distinct deviations in the experimental results.This method completely uses human body channel to transmit low-power signals,and has good anti-interference perform-ance.However,for a long time,the channel research of GCIBC has not determined a set of recog-nized and accurate measurement methods and standards.However,few researcher analyze the dif-ferences and principles between different measurement methods.This paper aims at three com-monly used measurement methods.Abstract the equivalent schematic diagrams of three measure-ment schemes:direct measurement,using high-precision differential probe and using balun.The sig-nal return paths generated by three measurement methods are analyzed,the impedance matching and energy transmission efficiency are compared.According to the analysis,the empirical expres-sions in different cases are listed.The mechanism between the channel measurement results gener-ated by the set experimental configuration and the comparison experiment is clarified.The compar-ison experiment with complete floating measurement is carried out as a reference to verify the pro-posed opinion.The measurement error caused by different experimental configurations is verified.This study can provide a reference opinions for the experimental error analysis of GCIBC research in the future,and provide suggestions for the selection of experimental configuration.
基金the National Natural Science Found-ation of China(U1505251)the International Cooperation Project in Fujian Province(2021I0005)Project of Edu-cation Department of Fujian Province(JAT200051).
文摘Intrabody communication(IBC)technology is becoming progressively more standard-ized due to its low power consumption and high security features incorporated into the third phys-ical layer of the IEEE 802.15.6 standard.Even then,there are still many challenges in normalizing the measurement issues of IBC.A major concern that should not be overlooked is the electrodes in the IBC,especially the popular use of gel electrodes.In the channel measurements,gel electrodes are commonly employed to improve the signal-to-noise ratio and prevent electrodes from falling off.In this paper,a comparative study of the electrical properties of gel was investigated during the measurement of human channel characteristics and to clarify the differences of them.Firstly,the basis of electrostatic field pole plate measurements and electromagnetic theory were introduced to interpretate how the relative permittivity and conductivity of different gels will influence the meas-urement results.Then the in vivo experiments with different gel or dry electrodes were performed to compare the differences induced by the gel.The results indicate that the influence of the gel on the human channel measurement is mainly concentrated below 400 kHz(the attenuation is re-duced by 16.7 dB on average),and the stability of the permittivity and conductivity of the gel has a direct impact on the stability of its measurement of the human channel.This result may provide a meaningful reference for the standardization of electrode usage in IBC.
