To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy ...To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy for up to 72 h after scald injury. The levels of tumor necrosis factor.(TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8) were determined by using the enzyme-linked immunosorbent assay (ELISA). Results: The levels of TNFα,IL-6 and IL-8 in plasma and SEF increased in the early phase of scald injury. Furthermore, the contents of TNFα， IL-6 and IL-8 in SEF were higher than those in plasma.Conclusion: The findings indicate that the subeschar edema fluid is a biologically active reservoir, and is an important participant in the postburn pathophysiologic process.展开更多
文摘To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy for up to 72 h after scald injury. The levels of tumor necrosis factor.(TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8) were determined by using the enzyme-linked immunosorbent assay (ELISA). Results: The levels of TNFα,IL-6 and IL-8 in plasma and SEF increased in the early phase of scald injury. Furthermore, the contents of TNFα， IL-6 and IL-8 in SEF were higher than those in plasma.Conclusion: The findings indicate that the subeschar edema fluid is a biologically active reservoir, and is an important participant in the postburn pathophysiologic process.
