BACKGROUND: Chemokines have strong chemoattractant effects and are involved in a variety of immune and inflammatory reactions, such as attracting activated T lymphocytes, neutrophils, monocytes and natural killer cell...BACKGROUND: Chemokines have strong chemoattractant effects and are involved in a variety of immune and inflammatory reactions, such as attracting activated T lymphocytes, neutrophils, monocytes and natural killer cells via the pathway of G protein-coupled receptors to sites of inflammatory injury and contribute to wound repair. This investigation was designed to assess the levels of chemokine interferon-gamma inducible protein-10 (IP-10) and IP-10 mRNA, and the relationship between IP-10 mRNA and HBV-DNA and alanine aminotransferase (ALT) in patients with chronic hepatitis B. METHODS: The levels of IP-10 mRNA in peripheral blood mononuclear cells (PBMCs) were kinetically detected by real-time polymerase chain reaction (PCR). The rate of chemokine/GAPDH was regarded as the extreme level of chemokine. The level of IP-10 in serum was measured by enzyme linked immunosorbent assay (ELISA), and the expression of IP-10 in hepatic biopsy tissue was detected by streptavidin-peroxidase (SP) immunohistochemistry. RESULTS: The level of IP-10 mRNA in the PBMCs of patients was 0.7387 +/- 0.0768 (lg cDNA/lg GAPDH); it was significantly higher in patients with chronic hepatitis B than that in normal controls (P<0.001). The level of IP-10 in the serum of patients was 660.9 +/- 75.5 pg/ml. There was a significant difference between patients with chronic hepatitis B and normal controls (P<0.05). In patients with chronic hepatitis B, the level of IP-10 mRNA in PBMCs was correlated with the IP-10 plasma level (r=0.7312, P<0.001), and the IP-10 plasma level was fairly correlated with the levels of ALT and HBV-DNA plasma (r=0.7235, P<0.001; r=0.7371, P<0.001). IP-10 was found by immunohistochemical analysis to be selectively upregulated on sinusoidal endothelium. CONCLUSIONS: The expression of IP-10 mRNA in PBMCs, IP-10 plasma concentration and the expression of IP-10 in sinusoidal endothelium are all high in patients with chronic hepatitis B. Chemokine IP-10 may play an important role in trafficking inflammatory cells to the local foc展开更多
BACKGROUND: Post-hepatitic cirrhosis is regarded as common and severe form of liver damage. Interferon gamma-inducible protein 10 (IP-10), a member of the non-ELR (glutamic-leucine-arginine) motif CXC chemokine family...BACKGROUND: Post-hepatitic cirrhosis is regarded as common and severe form of liver damage. Interferon gamma-inducible protein 10 (IP-10), a member of the non-ELR (glutamic-leucine-arginine) motif CXC chemokine family, has recently been shown to recruit and activate specific subsets of leukocytes to sites of inflammation or an immune response during the development of hepatic cirrhosis. However, the effects of IP-10 and IP-10 mRNA on inflammatory infiltration at local sites and in the peripheral blood of patients with post-hepatitic cirrhosis as well as their relationship with viral load are still poorly defined. This study aimed to detect the relationship between the expression of IP-10 in serum, IP-10 mRNA in peripheral blood mononuclear cells (PBMCs), and the levels of HBV DNA in the serum of patients, and to explore their role in the pathogenesis of cirrhosis. METHODS: Typical patients with cirrhosis after HBV infection were selected, and their serum IP-10 concentrations were evaluated with ELISA, the content of IP-10 mRNA in PBMCs was measured by real-time PCR, and the load of HBV DNA in serum and PBMCs was assessed by semi-quantitative analysis of gel imaging. RESULTS: The levels of IP-10 in serum and IP-10 mRNA in PBMCs of patients with cirrhosis were 299.9 +/- 77.2 pg/ml and 0.7500 +/- 0.1495, respectively. They were higher than those of controls (P<0.05) and also increased in the HBV DNA(+) groups (P<0.05, P<0.01) to 343.0 +/- 80.3 pg/ml and 0.8465 +/- 0.1528, respectively. The levels of IP-10 in serum and IP-10 mRNA in PBMCs were clearly correlated with the load of HBV DNA (P<0.01). CONCLUSIONS: The levels of IP-10 and IP-10 mRNA in the peripheral blood of patients with cirrhosis increase are closely correlated with the load of HBV DNA in serum, and play a key role in the progression of post-hepatitic cirrhosis. (Hepatobiliary Pancreat Dis Int 2010; 9: 280-286)展开更多
目的系统评价γ干扰素诱导蛋白10(IP-10)对活动性结核和潜伏性结核感染的鉴别诊断价值。方法检索PubMed、EMBASE、Cochrane图书馆和Web of science数据库,检索时间截止2019年3月,纳入英文发表IP-10对活动性结核和潜伏性结核感染的鉴别...目的系统评价γ干扰素诱导蛋白10(IP-10)对活动性结核和潜伏性结核感染的鉴别诊断价值。方法检索PubMed、EMBASE、Cochrane图书馆和Web of science数据库,检索时间截止2019年3月,纳入英文发表IP-10对活动性结核和潜伏性结核感染的鉴别诊断试验研究,2名研究人员进行数据提取和质量评价后,采用stata14.0软件进行统计分析。结果共纳入10篇文献,14个研究共773例患者,Meta分析结果显示合并敏感度、合并特异度、合并阳性似然比、阴性似然比、诊断比值比和ROC曲线下面积依次0.80(95%CI:0.71~0.87)、0.90(95%CI:0.81~0.95)、7.97(95%CI:4.07~15.61)、0.22(95%CI:0.15~0.33)、35.97(95%CI:14.93~86.65)和0.91(95%CI:0.88~0.93)。Deek′s漏斗图提示存在发表偏倚的可能性较小。结论IP-10鉴别活动性肺结核和潜伏性结核感染具有较高的敏感度和特异度。展开更多
目的:研究SARS冠状病毒S蛋白诱导呼吸道上皮细胞合成释放IP-10(interferon-gamma induc-ible protein 10)的信号分子机制。方法:通过基因芯片检测SARS冠状病毒的S蛋白作用于人支气管上皮细胞16HBE后信号通路基因表达谱的变化;采纳RT-PCR...目的:研究SARS冠状病毒S蛋白诱导呼吸道上皮细胞合成释放IP-10(interferon-gamma induc-ible protein 10)的信号分子机制。方法:通过基因芯片检测SARS冠状病毒的S蛋白作用于人支气管上皮细胞16HBE后信号通路基因表达谱的变化;采纳RT-PCR、EMSA、Western blotting等方法进一步分析JAK-STAT通路中信号分子的磷酸化、IRF-1和IP-10基因表达的变化及其相应信号分子抑制剂对表达水平的影响。结果:S蛋白作用于人支气管上皮细胞16HBE诱导了JAK-STAT信号通路涉及的重要转录因子基因IRF-1的表达,该信号通路的转录因子STAT1在刺激后15 min发生磷酸化,2 h即可检出IP-10基因的表达,IP-10的表达可以完全被STAT1、JAK2抑制剂阻断。EMSA显示:支气管上皮细胞在S蛋白的作用下,其核蛋白能够特异性与ISRE和GASDNA基序相结合,而不能与NF-κB的DNA基序相结合。结论:SARS-CoV的S蛋白通过激活JAK-STAT信号转导通路诱导IP-10在宿主细胞的生成。提示病毒诱导的JAK-STAT信号通路激活在病毒感染相关的急性肺损伤发生中具有重要地位。展开更多
为检测SLE患者外周血γ干扰素诱导蛋白10(interferon gamma-induced protein 10,IP-10)的表达情况并探索其临床意义及调控机制,采集上海交通大学医学院附属仁济医院SLE患者和健康体检者外周血,ELISA检测血清中IP-10和IFN-α水平。结果显...为检测SLE患者外周血γ干扰素诱导蛋白10(interferon gamma-induced protein 10,IP-10)的表达情况并探索其临床意义及调控机制,采集上海交通大学医学院附属仁济医院SLE患者和健康体检者外周血,ELISA检测血清中IP-10和IFN-α水平。结果显示,IP-10在SLE患者血清中的水平明显高于对照者(P<0.05);同时根据SLEDAI评分将患者分为无活动组(0~5分)、低活动度组(5~10分)和高活动度组(10分以上)后,发现SLE患者血清中IP-10水平与SLEDAI评分呈明显正相关(r=0.5703,P<0.05)。FACS检测外周血IP-10+细胞类型,发现高活动度组中IP-10+B细胞、NK细胞和单核细胞百分比明显高于低活动度组和正常对照组。用IFN-α刺激SLE患者和健康人PBMC,发现两组培养上清中IP-10水平明显高于未刺激组,但两组间差异无统计学意义(P>0.05);其中IP-10+B细胞百分比也显著升高,并且患者组升高更明显(均P<0.05)。上述结果提示SLE患者中B细胞在IFN-α作用下直接活化产生抗体的同时,也可通过分泌IP-10参与病理性炎症反应,这揭示了B细胞参与SLE发生发展的新机制。展开更多
HIV感染者即使接受了规律有效的抗病毒治疗,其体内仍然存在慢性长期的免疫激活,这已被证明是引起许多HIV相关非AIDS并发症的重要原因。其中固有免疫激活系统产生的趋化因子干扰素-γ诱导蛋白10(interferon gammainduced protein 10, IP...HIV感染者即使接受了规律有效的抗病毒治疗,其体内仍然存在慢性长期的免疫激活,这已被证明是引起许多HIV相关非AIDS并发症的重要原因。其中固有免疫激活系统产生的趋化因子干扰素-γ诱导蛋白10(interferon gammainduced protein 10, IP10)近年来在HIV研究领域受到更多的关注。IP10是一种由干扰素刺激基因产生的趋化蛋白,在HIV感染者疾病进展,与HIV感染者体内的适应性免疫系统相互作用,影响病毒储存库,引起非AIDS相关并发症方面起到重要作用。降低IP10水平或可为HIV感染者的治疗提供新的思路,为AIDS的功能性治愈奠定基础。展开更多
基金a grant from the Nature Science Foundation of the Department of Education of Anhui Province (No. 2007kj019A).
文摘BACKGROUND: Chemokines have strong chemoattractant effects and are involved in a variety of immune and inflammatory reactions, such as attracting activated T lymphocytes, neutrophils, monocytes and natural killer cells via the pathway of G protein-coupled receptors to sites of inflammatory injury and contribute to wound repair. This investigation was designed to assess the levels of chemokine interferon-gamma inducible protein-10 (IP-10) and IP-10 mRNA, and the relationship between IP-10 mRNA and HBV-DNA and alanine aminotransferase (ALT) in patients with chronic hepatitis B. METHODS: The levels of IP-10 mRNA in peripheral blood mononuclear cells (PBMCs) were kinetically detected by real-time polymerase chain reaction (PCR). The rate of chemokine/GAPDH was regarded as the extreme level of chemokine. The level of IP-10 in serum was measured by enzyme linked immunosorbent assay (ELISA), and the expression of IP-10 in hepatic biopsy tissue was detected by streptavidin-peroxidase (SP) immunohistochemistry. RESULTS: The level of IP-10 mRNA in the PBMCs of patients was 0.7387 +/- 0.0768 (lg cDNA/lg GAPDH); it was significantly higher in patients with chronic hepatitis B than that in normal controls (P<0.001). The level of IP-10 in the serum of patients was 660.9 +/- 75.5 pg/ml. There was a significant difference between patients with chronic hepatitis B and normal controls (P<0.05). In patients with chronic hepatitis B, the level of IP-10 mRNA in PBMCs was correlated with the IP-10 plasma level (r=0.7312, P<0.001), and the IP-10 plasma level was fairly correlated with the levels of ALT and HBV-DNA plasma (r=0.7235, P<0.001; r=0.7371, P<0.001). IP-10 was found by immunohistochemical analysis to be selectively upregulated on sinusoidal endothelium. CONCLUSIONS: The expression of IP-10 mRNA in PBMCs, IP-10 plasma concentration and the expression of IP-10 in sinusoidal endothelium are all high in patients with chronic hepatitis B. Chemokine IP-10 may play an important role in trafficking inflammatory cells to the local foc
基金supported by grants from the Natural Science Foundation of Anhui Province(090413138)the Natural Science Foundation of the Department of Education of Anhui Province(KJ2007A019,KJ2009A032,KJ2010A086)
文摘BACKGROUND: Post-hepatitic cirrhosis is regarded as common and severe form of liver damage. Interferon gamma-inducible protein 10 (IP-10), a member of the non-ELR (glutamic-leucine-arginine) motif CXC chemokine family, has recently been shown to recruit and activate specific subsets of leukocytes to sites of inflammation or an immune response during the development of hepatic cirrhosis. However, the effects of IP-10 and IP-10 mRNA on inflammatory infiltration at local sites and in the peripheral blood of patients with post-hepatitic cirrhosis as well as their relationship with viral load are still poorly defined. This study aimed to detect the relationship between the expression of IP-10 in serum, IP-10 mRNA in peripheral blood mononuclear cells (PBMCs), and the levels of HBV DNA in the serum of patients, and to explore their role in the pathogenesis of cirrhosis. METHODS: Typical patients with cirrhosis after HBV infection were selected, and their serum IP-10 concentrations were evaluated with ELISA, the content of IP-10 mRNA in PBMCs was measured by real-time PCR, and the load of HBV DNA in serum and PBMCs was assessed by semi-quantitative analysis of gel imaging. RESULTS: The levels of IP-10 in serum and IP-10 mRNA in PBMCs of patients with cirrhosis were 299.9 +/- 77.2 pg/ml and 0.7500 +/- 0.1495, respectively. They were higher than those of controls (P<0.05) and also increased in the HBV DNA(+) groups (P<0.05, P<0.01) to 343.0 +/- 80.3 pg/ml and 0.8465 +/- 0.1528, respectively. The levels of IP-10 in serum and IP-10 mRNA in PBMCs were clearly correlated with the load of HBV DNA (P<0.01). CONCLUSIONS: The levels of IP-10 and IP-10 mRNA in the peripheral blood of patients with cirrhosis increase are closely correlated with the load of HBV DNA in serum, and play a key role in the progression of post-hepatitic cirrhosis. (Hepatobiliary Pancreat Dis Int 2010; 9: 280-286)
文摘目的系统评价γ干扰素诱导蛋白10(IP-10)对活动性结核和潜伏性结核感染的鉴别诊断价值。方法检索PubMed、EMBASE、Cochrane图书馆和Web of science数据库,检索时间截止2019年3月,纳入英文发表IP-10对活动性结核和潜伏性结核感染的鉴别诊断试验研究,2名研究人员进行数据提取和质量评价后,采用stata14.0软件进行统计分析。结果共纳入10篇文献,14个研究共773例患者,Meta分析结果显示合并敏感度、合并特异度、合并阳性似然比、阴性似然比、诊断比值比和ROC曲线下面积依次0.80(95%CI:0.71~0.87)、0.90(95%CI:0.81~0.95)、7.97(95%CI:4.07~15.61)、0.22(95%CI:0.15~0.33)、35.97(95%CI:14.93~86.65)和0.91(95%CI:0.88~0.93)。Deek′s漏斗图提示存在发表偏倚的可能性较小。结论IP-10鉴别活动性肺结核和潜伏性结核感染具有较高的敏感度和特异度。
文摘为检测SLE患者外周血γ干扰素诱导蛋白10(interferon gamma-induced protein 10,IP-10)的表达情况并探索其临床意义及调控机制,采集上海交通大学医学院附属仁济医院SLE患者和健康体检者外周血,ELISA检测血清中IP-10和IFN-α水平。结果显示,IP-10在SLE患者血清中的水平明显高于对照者(P<0.05);同时根据SLEDAI评分将患者分为无活动组(0~5分)、低活动度组(5~10分)和高活动度组(10分以上)后,发现SLE患者血清中IP-10水平与SLEDAI评分呈明显正相关(r=0.5703,P<0.05)。FACS检测外周血IP-10+细胞类型,发现高活动度组中IP-10+B细胞、NK细胞和单核细胞百分比明显高于低活动度组和正常对照组。用IFN-α刺激SLE患者和健康人PBMC,发现两组培养上清中IP-10水平明显高于未刺激组,但两组间差异无统计学意义(P>0.05);其中IP-10+B细胞百分比也显著升高,并且患者组升高更明显(均P<0.05)。上述结果提示SLE患者中B细胞在IFN-α作用下直接活化产生抗体的同时,也可通过分泌IP-10参与病理性炎症反应,这揭示了B细胞参与SLE发生发展的新机制。
文摘HIV感染者即使接受了规律有效的抗病毒治疗,其体内仍然存在慢性长期的免疫激活,这已被证明是引起许多HIV相关非AIDS并发症的重要原因。其中固有免疫激活系统产生的趋化因子干扰素-γ诱导蛋白10(interferon gammainduced protein 10, IP10)近年来在HIV研究领域受到更多的关注。IP10是一种由干扰素刺激基因产生的趋化蛋白,在HIV感染者疾病进展,与HIV感染者体内的适应性免疫系统相互作用,影响病毒储存库,引起非AIDS相关并发症方面起到重要作用。降低IP10水平或可为HIV感染者的治疗提供新的思路,为AIDS的功能性治愈奠定基础。
