Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a...Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a water-soluble redox initiating system consisting of ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylenediamine (TEM- ED). Gelation time of PEG diacrylate macromer in phos- phate buffer saline solution was first adjusted to guarantee that the examined initiator concentrations are sufficiently high to trigger polymerization of macromers. NIH/3T3 fi- broblasts were employed to examine cytotoxicity via MTT measurements and optical microscopic observations. It has been found that the combined APS/TEMED system exhibits negative cooperative effect, for the underlying cytotoxicity is even lower than that of APS or TEMED at certain concen- trations.展开更多
基金the Nationa1 Natura1 Science Foundation of China(Grant Nos.2O174006,20221402,30271293 , 20374015)the Key Grant of the Chinese Ministry of Education(Grant No.305004)+3 种基金the Award Foundation for Young Teachers from the Ministry of Educationthe 973 Project(Grant No.G1999054306-03)the 863 Project(Grant No.2004AA215170) the Science and Technology Developing Foundation of Shanghai (Grant Nos.04JC14019,02DJ14017).
文摘Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a water-soluble redox initiating system consisting of ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylenediamine (TEM- ED). Gelation time of PEG diacrylate macromer in phos- phate buffer saline solution was first adjusted to guarantee that the examined initiator concentrations are sufficiently high to trigger polymerization of macromers. NIH/3T3 fi- broblasts were employed to examine cytotoxicity via MTT measurements and optical microscopic observations. It has been found that the combined APS/TEMED system exhibits negative cooperative effect, for the underlying cytotoxicity is even lower than that of APS or TEMED at certain concen- trations.
