目的评价酒精灭活在重建恶性骨肿瘤骨缺损中的疗效,并分析影响疗效的相关因素。方法回顾性分析1995年1月至2013年6月,我科对53例恶性骨肿瘤患者进行酒精灭活再植治疗。平均年龄17.7（9～49）岁。其中股骨下段23例,股骨中段2例,胫骨上段2...目的评价酒精灭活在重建恶性骨肿瘤骨缺损中的疗效,并分析影响疗效的相关因素。方法回顾性分析1995年1月至2013年6月,我科对53例恶性骨肿瘤患者进行酒精灭活再植治疗。平均年龄17.7（9～49）岁。其中股骨下段23例,股骨中段2例,胫骨上段20例,胫骨下段3例,肱骨上段3例,骶骨、髂骨各1例;Enneking分期II b期48例,III期5例;骨肉瘤44例,尤文肉瘤6例,横纹肌肉瘤、软骨肉瘤、非霍奇金淋巴瘤各1例。术后疗效评价采用（musculoskeletal tumor society system,MSTS）功能评分和（international society of limb salvage,ISOLS）影像评分。统计并分析各因素与术后功能、生存率的相关性。结果随访13～216（平均55）个月。广泛切除39例,边缘切除14例。灭活回植的瘤段平均长度为16.3（5～26）cm。II b期的48例中11例出现肿瘤复发,肺转移9例,转移率18.8%,6例死亡,5例带瘤生存;44例骨肉瘤中7例出现肿瘤复发,肺转移6例,转移率13.6%,术后3年生存35例。5年生存率为42.5%,其中骨肉瘤5年生存率为54.5%。30例灭活骨得以长期存在,其3年总生存率57%（30/53）,保肢率68%（36/53）。术后切口感染4例（7.6%）,骨折、内固定断裂5例（9.4%,5/53）,内固定断裂2例（3.8%,2/53）。灭活骨延迟愈合、不愈合共8例（15.1%,8/53）。MSTS功能评分19～33（平均27）分;ISOLS影像评分22～31（平均26）分。单因素分析提示：（1）灭活瘤段长度与骨痂出现率、骨痂出现时间、术后复发率、总生存率、MSTS评分、ISOLS评分均具有相关性（P〈0.05）;（2）肿瘤分期与术后复发率具有相关性（P〈0.05）;（3）是否保留关节与MSTS评分具有相关性（P〈0.05）;（4）病程、肿瘤分期（P=0.026）、发病部位与患者3年生存率具有相关性（P〈0.05）。多因素分析提示：灭活瘤段长度、病程长短、肿瘤分期是影响总生存率的独立因素（P〈0.05）。结论酒精灭活瘤段骨治�展开更多
A series of new O6-BG derivatives （14-21,23-30） were synthesized as inactivators of O6-Alkylguanine-DNA alkyltransferase （AGT）, and their ability to inhibit AGT was preliminary evaluated by MTT with O6-BG as the c...A series of new O6-BG derivatives （14-21,23-30） were synthesized as inactivators of O6-Alkylguanine-DNA alkyltransferase （AGT）, and their ability to inhibit AGT was preliminary evaluated by MTT with O6-BG as the control. The result suggested compound 30 displayed a higher activity than O6-BG.展开更多
In sewage sludge composting, adding ash from stove （FA20） inactivated active Cu effectively and the results were significantly better than control. For Zn, Mn, Pb and Cd, satisfying results was,achieved by adding as...In sewage sludge composting, adding ash from stove （FA20） inactivated active Cu effectively and the results were significantly better than control. For Zn, Mn, Pb and Cd, satisfying results was,achieved by adding ash from stove and phosphorite powder （FA20, PR10）, phosphorite powder and lime （PR15, CL10）, ash from stove and phosphorite powder （FA10, PR10）, and ash from stove and peat （FA20, PT 10）. Based on their effects of inactivating available forms of heavy metals, ash from stove, phosphorite powder and peat were supposed to be effective inactivators with optimum percentages of 20%, 15% and 10% （FA20, PR15, PT10）.展开更多
OBJECTIVE: To assess complement-mediated myocardial injury on isolated guinea pig working hearts and cardioprotective effects of CD59. METHODS: Using a modified Langendorff apparatus, isolated guinea-pig working heart...OBJECTIVE: To assess complement-mediated myocardial injury on isolated guinea pig working hearts and cardioprotective effects of CD59. METHODS: Using a modified Langendorff apparatus, isolated guinea-pig working hearts were perfused with a modified Krebs Henseleit buffer containing 3% heat-inactivated human plasma and zymosan (IPZ) (control) (n = 10), 3% normal human plasma and zymosan (NPZ) (n = 10), or 3% normal human plasma and zymosan and 1.5 microg/ml CD59 (NPZC) (n = 10), respectively. Epicardial electrocardiogram (ECG), cardiac output (CO), coronary arterial flow (CF), maximum left ventricular developed pressure (LVP(max)), maximum left ventricular developed pressure increase rate (+ dp/dt(max)), maximum left ventricular developed pressure decrease rate (- dp/dt(max)) and heart rate (HR) were recorded at 0, 15, 30, 45 and 60 min of treatment. After the experiment, immunohistochemical examination was performed to detect the presence of C3a or C5b-9 in the myocardium of the isolated hearts. RESULTS: Compared the IPZ group, hearts treated with NPZ showed a slight depression on ST segments of epicardial ECG at 15 min, a significant elevation between 30 min to 60 min, a decrease in CF, CO, LVP(max), + dp/dt(max) and - dp/dt(max), and an increase in HR at 15 min. The observed alterations in CF, CO, LVP(max), + dp/dt(max) and - dp/dt(max) remained decreased, while the HR remained increased until the end of the protocol. The all above parameters of hearts treated with NPZC were similar to the control group (IPZ) at any given time. Immunohistochemical examination showed positive signals of C3a and C5b-9 in the myocardium of hearts treated with NPZ. C3a was positive in NPZC, and C3a and C5b-9 were negative in IPZ. CONCLUSIONS: Activated human complements directly damage isolated guinea pig working hearts, and CD59 offers a significant protection against the injuries.展开更多
文摘目的评价酒精灭活在重建恶性骨肿瘤骨缺损中的疗效,并分析影响疗效的相关因素。方法回顾性分析1995年1月至2013年6月,我科对53例恶性骨肿瘤患者进行酒精灭活再植治疗。平均年龄17.7（9～49）岁。其中股骨下段23例,股骨中段2例,胫骨上段20例,胫骨下段3例,肱骨上段3例,骶骨、髂骨各1例;Enneking分期II b期48例,III期5例;骨肉瘤44例,尤文肉瘤6例,横纹肌肉瘤、软骨肉瘤、非霍奇金淋巴瘤各1例。术后疗效评价采用（musculoskeletal tumor society system,MSTS）功能评分和（international society of limb salvage,ISOLS）影像评分。统计并分析各因素与术后功能、生存率的相关性。结果随访13～216（平均55）个月。广泛切除39例,边缘切除14例。灭活回植的瘤段平均长度为16.3（5～26）cm。II b期的48例中11例出现肿瘤复发,肺转移9例,转移率18.8%,6例死亡,5例带瘤生存;44例骨肉瘤中7例出现肿瘤复发,肺转移6例,转移率13.6%,术后3年生存35例。5年生存率为42.5%,其中骨肉瘤5年生存率为54.5%。30例灭活骨得以长期存在,其3年总生存率57%（30/53）,保肢率68%（36/53）。术后切口感染4例（7.6%）,骨折、内固定断裂5例（9.4%,5/53）,内固定断裂2例（3.8%,2/53）。灭活骨延迟愈合、不愈合共8例（15.1%,8/53）。MSTS功能评分19～33（平均27）分;ISOLS影像评分22～31（平均26）分。单因素分析提示：（1）灭活瘤段长度与骨痂出现率、骨痂出现时间、术后复发率、总生存率、MSTS评分、ISOLS评分均具有相关性（P〈0.05）;（2）肿瘤分期与术后复发率具有相关性（P〈0.05）;（3）是否保留关节与MSTS评分具有相关性（P〈0.05）;（4）病程、肿瘤分期（P=0.026）、发病部位与患者3年生存率具有相关性（P〈0.05）。多因素分析提示：灭活瘤段长度、病程长短、肿瘤分期是影响总生存率的独立因素（P〈0.05）。结论酒精灭活瘤段骨治�
基金research Grant from Beijing Science and Technology Program (No.00004105040311).
文摘A series of new O6-BG derivatives （14-21,23-30） were synthesized as inactivators of O6-Alkylguanine-DNA alkyltransferase （AGT）, and their ability to inhibit AGT was preliminary evaluated by MTT with O6-BG as the control. The result suggested compound 30 displayed a higher activity than O6-BG.
文摘In sewage sludge composting, adding ash from stove （FA20） inactivated active Cu effectively and the results were significantly better than control. For Zn, Mn, Pb and Cd, satisfying results was,achieved by adding ash from stove and phosphorite powder （FA20, PR10）, phosphorite powder and lime （PR15, CL10）, ash from stove and phosphorite powder （FA10, PR10）, and ash from stove and peat （FA20, PT 10）. Based on their effects of inactivating available forms of heavy metals, ash from stove, phosphorite powder and peat were supposed to be effective inactivators with optimum percentages of 20%, 15% and 10% （FA20, PR15, PT10）.
基金theScientificResearchFoundationoftheMinistryofHealth ,China (No .96 1115 )
文摘OBJECTIVE: To assess complement-mediated myocardial injury on isolated guinea pig working hearts and cardioprotective effects of CD59. METHODS: Using a modified Langendorff apparatus, isolated guinea-pig working hearts were perfused with a modified Krebs Henseleit buffer containing 3% heat-inactivated human plasma and zymosan (IPZ) (control) (n = 10), 3% normal human plasma and zymosan (NPZ) (n = 10), or 3% normal human plasma and zymosan and 1.5 microg/ml CD59 (NPZC) (n = 10), respectively. Epicardial electrocardiogram (ECG), cardiac output (CO), coronary arterial flow (CF), maximum left ventricular developed pressure (LVP(max)), maximum left ventricular developed pressure increase rate (+ dp/dt(max)), maximum left ventricular developed pressure decrease rate (- dp/dt(max)) and heart rate (HR) were recorded at 0, 15, 30, 45 and 60 min of treatment. After the experiment, immunohistochemical examination was performed to detect the presence of C3a or C5b-9 in the myocardium of the isolated hearts. RESULTS: Compared the IPZ group, hearts treated with NPZ showed a slight depression on ST segments of epicardial ECG at 15 min, a significant elevation between 30 min to 60 min, a decrease in CF, CO, LVP(max), + dp/dt(max) and - dp/dt(max), and an increase in HR at 15 min. The observed alterations in CF, CO, LVP(max), + dp/dt(max) and - dp/dt(max) remained decreased, while the HR remained increased until the end of the protocol. The all above parameters of hearts treated with NPZC were similar to the control group (IPZ) at any given time. Immunohistochemical examination showed positive signals of C3a and C5b-9 in the myocardium of hearts treated with NPZ. C3a was positive in NPZC, and C3a and C5b-9 were negative in IPZ. CONCLUSIONS: Activated human complements directly damage isolated guinea pig working hearts, and CD59 offers a significant protection against the injuries.
