Objective:Laboratory capacity for Chlamydia trachomatis (C.trachomatis) detection is important in the diagnosis and treatment of C.trachomatis infection,appropriate clinical management of patients,and providing eviden...Objective:Laboratory capacity for Chlamydia trachomatis (C.trachomatis) detection is important in the diagnosis and treatment of C.trachomatis infection,appropriate clinical management of patients,and providing evidence for prevention programs.We conducted this study to assess laboratory capabilities for detecting C.trachomatis in China by analysis of external quality assessment (EQA) results from 2013 to 2018.Methods:Overall,310/1,048 (29.58%) laboratories at national sexually transmitted disease (STD) sentinel site with 252-272 laboratories per time participated in six times of EQA.Each laboratory was requested to test the samples from EQA organization by the common method used,and the test results were reported to EQA organization for assessment.Z test and multinomial logistic regression analyses were used for data analyses.Results:Immunochromatographic test,nucleic acid amplification test (NAAT),and ELISA were used and accounted for 76.69%,21.54%,1.77%,respectively of all participating laboratories from 2013 to 2018.The total specificity for negative samples was 94.76%,the sensitivity for positive samples with medium and high concentration of C.trachomatis samples were 94.31% and 95.51%,respectively,but the sensitivity for sample with low concentration of Co trachomatis was 36.89%,and the immunochromatographic test had the worst sensitivity for detection of this sample (21.17% [95% C/s:18.93%-23.60%]) among the three methods.Three factors were found to be significantly associated with the sensitivity of the low-concentration sample:the location of laboratories (East China:adjusted odds ratio [AOR] =2.98,95% C/s:1.69-5.25,P < 0.05;South China:AOR =3.34,95% CIs:1.38-5.48,P < 0.05;Southwest China:AOR=2.75,95% CIs:1.37-5.48,P<0.05,as compared with Northwest China);the types of hospitals (prevention and control agencies:AOR =0.56,95% CIs:0.40-0.80,P < 0.05,as compared with general hospitals);and the method used (NAAT:AOR=46.99,95% CIs:28.49-77.48,P< 0.050;ELISA:AOR=5.42,95% CIS:2.40-12.25,P<0.05,as compared with i展开更多
免疫层析试纸条技术(Immunochromatographic Test Strip,ICTS)结合了色谱分析的分离能力和免疫分析的特异性,具有操作简单,检测快速以及价格低廉的特点,已成为食品安全快速检测领域研究的热点。传统的ICTS是以胶体金作为信号标记材料,...免疫层析试纸条技术(Immunochromatographic Test Strip,ICTS)结合了色谱分析的分离能力和免疫分析的特异性,具有操作简单,检测快速以及价格低廉的特点,已成为食品安全快速检测领域研究的热点。传统的ICTS是以胶体金作为信号标记材料,但是胶体金试纸条检测灵敏度较低,只适用于定性和半定量检测。为了提升试纸条的检测性能,研究者做了大量的努力。本论文介绍了传统的胶体金试纸条的检测基本原理,并对近年来开发的试纸条检测新技术进行综述,同时也提出该项技术目前所存在的局限性并提出了未来的发展方向,以期为试纸条的进一步开发利用提供一定的文献支持。展开更多
We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was ...We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.展开更多
AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in ad...AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. METHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTM ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection.展开更多
基金supported by the Chinese Academy of Medical Sciences Initiative for Innovative Medicine (2016I2M-3–021)
文摘Objective:Laboratory capacity for Chlamydia trachomatis (C.trachomatis) detection is important in the diagnosis and treatment of C.trachomatis infection,appropriate clinical management of patients,and providing evidence for prevention programs.We conducted this study to assess laboratory capabilities for detecting C.trachomatis in China by analysis of external quality assessment (EQA) results from 2013 to 2018.Methods:Overall,310/1,048 (29.58%) laboratories at national sexually transmitted disease (STD) sentinel site with 252-272 laboratories per time participated in six times of EQA.Each laboratory was requested to test the samples from EQA organization by the common method used,and the test results were reported to EQA organization for assessment.Z test and multinomial logistic regression analyses were used for data analyses.Results:Immunochromatographic test,nucleic acid amplification test (NAAT),and ELISA were used and accounted for 76.69%,21.54%,1.77%,respectively of all participating laboratories from 2013 to 2018.The total specificity for negative samples was 94.76%,the sensitivity for positive samples with medium and high concentration of C.trachomatis samples were 94.31% and 95.51%,respectively,but the sensitivity for sample with low concentration of Co trachomatis was 36.89%,and the immunochromatographic test had the worst sensitivity for detection of this sample (21.17% [95% C/s:18.93%-23.60%]) among the three methods.Three factors were found to be significantly associated with the sensitivity of the low-concentration sample:the location of laboratories (East China:adjusted odds ratio [AOR] =2.98,95% C/s:1.69-5.25,P < 0.05;South China:AOR =3.34,95% CIs:1.38-5.48,P < 0.05;Southwest China:AOR=2.75,95% CIs:1.37-5.48,P<0.05,as compared with Northwest China);the types of hospitals (prevention and control agencies:AOR =0.56,95% CIs:0.40-0.80,P < 0.05,as compared with general hospitals);and the method used (NAAT:AOR=46.99,95% CIs:28.49-77.48,P< 0.050;ELISA:AOR=5.42,95% CIS:2.40-12.25,P<0.05,as compared with i
文摘免疫层析试纸条技术(Immunochromatographic Test Strip,ICTS)结合了色谱分析的分离能力和免疫分析的特异性,具有操作简单,检测快速以及价格低廉的特点,已成为食品安全快速检测领域研究的热点。传统的ICTS是以胶体金作为信号标记材料,但是胶体金试纸条检测灵敏度较低,只适用于定性和半定量检测。为了提升试纸条的检测性能,研究者做了大量的努力。本论文介绍了传统的胶体金试纸条的检测基本原理,并对近年来开发的试纸条检测新技术进行综述,同时也提出该项技术目前所存在的局限性并提出了未来的发展方向,以期为试纸条的进一步开发利用提供一定的文献支持。
基金supported by the National Natural Science Foundation of China(22236002).
文摘We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.
文摘AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. METHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTM ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection.
