Das Zytomegalie-Virus ist angesichts seiner hohen Pravalenz das haufigste interuterin ubertragene Virus. Zur Diagnose der Zytomegalie-Virus-Infektion stehen mehrere Moglichkeiten zur Verfugun, z.B. die Viruskultur aus...Das Zytomegalie-Virus ist angesichts seiner hohen Pravalenz das haufigste interuterin ubertragene Virus. Zur Diagnose der Zytomegalie-Virus-Infektion stehen mehrere Moglichkeiten zur Verfugun, z.B. die Viruskultur aus verschiedenen Materialen und die DNA-Hybridisierung. Die schnelle und zuerlassige Diagnostik ist die Bestimmung von Zytomegalie-Virus-Antikorpern, naemlich quantitativer Nachweis von Zytomegalie-Virus-IgG-Antikorpern und qualitativer Nachweis von Zytomeglie-Virus Spezifischen IgM-Antikorpern. Der Nachweis von IgM-Antikorpern oder der Anstieg des IgG-Antikorpers bei einer Serokonversion sind Zeichen der aktiven Zytomegalie-Virus-Infektion, wonach diese Infektion gewertet werden kann. Um die Frage der Brauchbarkeit der methode fur den Zytomegalie-Virus-Antikorper-Nachweis zu beantworten und die Technik zum Nachweis von Zytomegalie-Virus-Antikorpern zu prufen, wurde in der Studie der Nachweis von Zytomegalie-Virus-IgG und IgM-Antikorpern aus filtergetrocknetem Blut durchgefuhrt. Dabei wurde festgestellt, da diese Filterpapier-Technik zum Nachweis von Zytomegalie-Virus-Antikorpern durchfuhrbar ist.展开更多
Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prov...Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.展开更多
文摘Das Zytomegalie-Virus ist angesichts seiner hohen Pravalenz das haufigste interuterin ubertragene Virus. Zur Diagnose der Zytomegalie-Virus-Infektion stehen mehrere Moglichkeiten zur Verfugun, z.B. die Viruskultur aus verschiedenen Materialen und die DNA-Hybridisierung. Die schnelle und zuerlassige Diagnostik ist die Bestimmung von Zytomegalie-Virus-Antikorpern, naemlich quantitativer Nachweis von Zytomegalie-Virus-IgG-Antikorpern und qualitativer Nachweis von Zytomeglie-Virus Spezifischen IgM-Antikorpern. Der Nachweis von IgM-Antikorpern oder der Anstieg des IgG-Antikorpers bei einer Serokonversion sind Zeichen der aktiven Zytomegalie-Virus-Infektion, wonach diese Infektion gewertet werden kann. Um die Frage der Brauchbarkeit der methode fur den Zytomegalie-Virus-Antikorper-Nachweis zu beantworten und die Technik zum Nachweis von Zytomegalie-Virus-Antikorpern zu prufen, wurde in der Studie der Nachweis von Zytomegalie-Virus-IgG und IgM-Antikorpern aus filtergetrocknetem Blut durchgefuhrt. Dabei wurde festgestellt, da diese Filterpapier-Technik zum Nachweis von Zytomegalie-Virus-Antikorpern durchfuhrbar ist.
文摘Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.
