目的分析过敏原特异性Ig E(sI g E)检测应用于过敏性鼻炎患儿的价值。方法从我院收治的过敏性鼻炎患儿中选取416例,对其血清中食入性与吸入性sI g E进行检测,对检测结果进行研究分析。结果 416例患儿至少出现了一种过敏原阳性反应,阳性...目的分析过敏原特异性Ig E(sI g E)检测应用于过敏性鼻炎患儿的价值。方法从我院收治的过敏性鼻炎患儿中选取416例,对其血清中食入性与吸入性sI g E进行检测,对检测结果进行研究分析。结果 416例患儿至少出现了一种过敏原阳性反应,阳性率为100%;吸入性过敏原属于各年龄段过敏性鼻炎患儿的主要过敏原类型,所占比例为76.44%。结论 sI g E检测应用于过敏性鼻炎患儿中的效果显著。展开更多
Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient ser...Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.展开更多
文摘目的分析过敏原特异性Ig E(sI g E)检测应用于过敏性鼻炎患儿的价值。方法从我院收治的过敏性鼻炎患儿中选取416例,对其血清中食入性与吸入性sI g E进行检测,对检测结果进行研究分析。结果 416例患儿至少出现了一种过敏原阳性反应,阳性率为100%;吸入性过敏原属于各年龄段过敏性鼻炎患儿的主要过敏原类型,所占比例为76.44%。结论 sI g E检测应用于过敏性鼻炎患儿中的效果显著。
基金supported by the National Key Research and Development Project (No.2020YFA0709900)the National Natural Science Foundation of China (Nos.81871265,81672508,81702617)+4 种基金Key University Science Research Project of Jiangsu Province (No.19KJA520005)Natural Science Basic Research Program of Shaanxi Province (No.2019JM-016)National Natural Science Foundation of Jiangxi Province (No.20202BABL206043)Science and Technology Project of Jiangxi Provincial Health Commission (No.20204495)Open Research Fund of Anhui Key Laboratory of Tobacco。
文摘Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.
