[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer(ITS region)of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DN...[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer(ITS region)of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.展开更多
基金Supported by National Science Foundation of China(30970187)~~
文摘[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer(ITS region)of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.