AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis...AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis (SAP) was induced by retrograde infusion of artificial bile juice into biliary pancreatic duct, and treated with YHI and HHI I intravenously. The protective effect was judged by the survival time and rate, serum amylase, serum interleukin 6, pancreatic microcirculation and pathological alteration. RESULTS Combined use of YHI and HHI I could markedly increase the rabbits′ 5 day survival rate after AP (83 3% in the treatment group and 33 3% in control). The serum amylase value ( ±s ) decreased to 1596 6U/L ± 760 50U/L in the 5th day from the high level ( 6320 83U/L ± 2614 12U/L ) in the 1st day after AP in the treatment group, while in the control group the amylase activity in the 5th day was 2095 0U/L ± 1081 87U/L , being significantly different from that before AP ( 837 17U/L ± 189 12U/L ). YHI and HHI I also obviously improved the pancreatic microcirculation and lowered the serum interleukin 6 level, one of the indices of severe pancreatitis. Pathological examination indicated all the changes typical for AP in YHI and HHI I treatment group were milder than those in the control. CONCLUSION YHI and HHI I used in combination might have protective effect against acute pancreatitis in rabbits.展开更多
文摘AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis (SAP) was induced by retrograde infusion of artificial bile juice into biliary pancreatic duct, and treated with YHI and HHI I intravenously. The protective effect was judged by the survival time and rate, serum amylase, serum interleukin 6, pancreatic microcirculation and pathological alteration. RESULTS Combined use of YHI and HHI I could markedly increase the rabbits′ 5 day survival rate after AP (83 3% in the treatment group and 33 3% in control). The serum amylase value ( ±s ) decreased to 1596 6U/L ± 760 50U/L in the 5th day from the high level ( 6320 83U/L ± 2614 12U/L ) in the 1st day after AP in the treatment group, while in the control group the amylase activity in the 5th day was 2095 0U/L ± 1081 87U/L , being significantly different from that before AP ( 837 17U/L ± 189 12U/L ). YHI and HHI I also obviously improved the pancreatic microcirculation and lowered the serum interleukin 6 level, one of the indices of severe pancreatitis. Pathological examination indicated all the changes typical for AP in YHI and HHI I treatment group were milder than those in the control. CONCLUSION YHI and HHI I used in combination might have protective effect against acute pancreatitis in rabbits.
文摘【目的】探讨手足口病不同临床分期（1、2、3、4期）肿瘤坏死因子（tumor necrosis factor ，TNF）、白介素-6（IL-6）及B型脑钠肽（BNP）的变化特点，为临床评估此类患者提供参考。【方法】选择2011年5月至2013年10月在本院就诊的手足口病患儿292例（其中1期156例，2期106例，3期20例，4期10例），于患儿入院24 h内抽血行TNF、IL-6、BNP检测。观察比较不同分期手足口病患儿细胞因子及BNP的变化特点。【结果】各期TNF、IL-6、BNP水平均较健康对照组明显增高，2期、3期患儿TNF、IL-6、BNP水平明显高于1期，4期的TNF、IL-6、BNP水平明显高于2期、3期水平，且差异相比较均有显著性（ P ＜0．05）。2、3期TNF、IL-6、BNP水平相比较差异无显著性（ P ＞0．05）。【结论】随着患儿病情加重，TNF、IL-6及BNP水平随之相应增高，TNF、IL-6及BN P水平可用于手足口病患儿病情评估的参考指标。
