AIM: Expression of heat shock proteins (HSPs) is frequently up-regulated in hepatocellular carcinoma (HCC), which evolves from dysplastic nodule (DN) and early HCC to advanced HCC. However, little is known about the d...AIM: Expression of heat shock proteins (HSPs) is frequently up-regulated in hepatocellular carcinoma (HCC), which evolves from dysplastic nodule (DN) and early HCC to advanced HCC. However, little is known about the differential expression of HSPs in multistep hepatocarcinogenesis. It was the purpose of this study to monitor the expression of HSPs in multistep hepatocarcinogenesis and to evaluate their prognostic significance in hepatitis B virus (HBV)related HCC.METHODS: Thirty-eight HCC and 19 DN samples were obtained from 52 hepatitis B surface antigen-positive Korean patients. Immunohistochemical and dot immunoblot analyses of HSP27, HSP60, HSP70, HSP90, glucoseregulated protein (GRP)78, and GRP94 were performed and their expression at different stages of HCC development was statistically analyzed.RESULTS: Expression of HSP27, HSP70, HSP90, GRP78, and GRP94 increased along with the stepwise progression of hepatocarcinogenesis. Strong correlation was found only in GRP78 (Spearman's r= 0.802). There was a positive correlation between the expressions of GRP78, GRP94, HSP90, or HSP70 and prognostic factors of HCC. Specifically, the expression of GRP78, GRP94, or HSP90 was associated significantly with vascular invasion and intrahepatic metastasis.CONCLUSION: The expressions of HSPs are commonly up-regulated in HBV-related HCCs and GRP78 might play an important role in the stepwise progression of HBVrelated hepatocarcinogenesis. GRP78, GRP94, and HSP90 may be important prognostic markers of HBV-related HCC, strongly suggesting vascular invasion and intrahepatic metastasis.展开更多
The distribution and biosynthesis sites of cathepsin B in Helicoverpa armigera through the developing stages were studied by means of Immunoblot and Northern Blot.The results of Immunoblot showed that cathepsin B exis...The distribution and biosynthesis sites of cathepsin B in Helicoverpa armigera through the developing stages were studied by means of Immunoblot and Northern Blot.The results of Immunoblot showed that cathepsin B existed in ovary,female and male adult fat body.The mRNA in larval fat body,female pupal fat body,male adult fat body,female adult fat body and ovary were detected using Northern Blot.This indicated that cathepsin B mRNA could be transcribed in these tissues.However there was no cathepsin B mRNA in larval epidermis and larval mid gut as also no distribution of this proteinase.Furthermore,we detected mRNA transcription in larval fat body,female pupal fat body using Northern Blot,but there were no cathepsin B distributed in these two issues accordingly in Immunoblot.This indicated that the mRNA in these two tissues could not be translated into cathepsin B,and there might be some kind of regulation in translation level.展开更多
基金Supported by the fund from the Korea Science and Engineering Foundation (Grant No. R01-2001-00098). Seung Oe Lim was supported by BK21 Research Fellowship from the Ministry of Education and Human Resources Development
文摘AIM: Expression of heat shock proteins (HSPs) is frequently up-regulated in hepatocellular carcinoma (HCC), which evolves from dysplastic nodule (DN) and early HCC to advanced HCC. However, little is known about the differential expression of HSPs in multistep hepatocarcinogenesis. It was the purpose of this study to monitor the expression of HSPs in multistep hepatocarcinogenesis and to evaluate their prognostic significance in hepatitis B virus (HBV)related HCC.METHODS: Thirty-eight HCC and 19 DN samples were obtained from 52 hepatitis B surface antigen-positive Korean patients. Immunohistochemical and dot immunoblot analyses of HSP27, HSP60, HSP70, HSP90, glucoseregulated protein (GRP)78, and GRP94 were performed and their expression at different stages of HCC development was statistically analyzed.RESULTS: Expression of HSP27, HSP70, HSP90, GRP78, and GRP94 increased along with the stepwise progression of hepatocarcinogenesis. Strong correlation was found only in GRP78 (Spearman's r= 0.802). There was a positive correlation between the expressions of GRP78, GRP94, HSP90, or HSP70 and prognostic factors of HCC. Specifically, the expression of GRP78, GRP94, or HSP90 was associated significantly with vascular invasion and intrahepatic metastasis.CONCLUSION: The expressions of HSPs are commonly up-regulated in HBV-related HCCs and GRP78 might play an important role in the stepwise progression of HBVrelated hepatocarcinogenesis. GRP78, GRP94, and HSP90 may be important prognostic markers of HBV-related HCC, strongly suggesting vascular invasion and intrahepatic metastasis.
文摘The distribution and biosynthesis sites of cathepsin B in Helicoverpa armigera through the developing stages were studied by means of Immunoblot and Northern Blot.The results of Immunoblot showed that cathepsin B existed in ovary,female and male adult fat body.The mRNA in larval fat body,female pupal fat body,male adult fat body,female adult fat body and ovary were detected using Northern Blot.This indicated that cathepsin B mRNA could be transcribed in these tissues.However there was no cathepsin B mRNA in larval epidermis and larval mid gut as also no distribution of this proteinase.Furthermore,we detected mRNA transcription in larval fat body,female pupal fat body using Northern Blot,but there were no cathepsin B distributed in these two issues accordingly in Immunoblot.This indicated that the mRNA in these two tissues could not be translated into cathepsin B,and there might be some kind of regulation in translation level.
