Insulin-like growth factor-II mRNA binding protein(igf2bp)is an mRNA binding protein that regulates the post-transcriptional fate of multiple transcripts.In this study,a novel igf2bp gene was identified in the razor c...Insulin-like growth factor-II mRNA binding protein(igf2bp)is an mRNA binding protein that regulates the post-transcriptional fate of multiple transcripts.In this study,a novel igf2bp gene was identified in the razor clam Sinonovacula constricta.The complete 2254 bp Sc-igf2bp cDNA consists of an open reading frame(ORF)encoding 610 amino acid(aa)residues.The deduced protein includes a single RNA recognition motif(RRM)and four K homology(KH)domains.Sc-igf2bp transcripts were expressed widely during different developmental stages and in adult tissues,and a 95 kDa recombinant pEGX-4T-1-Scigf2bp protein was expressed in Escherichia coli Rosetta(DE3)pLysS cells and purified using Glutathione Sepharose 4B resin.Activity was evaluated by GST pull-down assays,and mass spectrometry results indicated that Sc-igf2bp may participate in RNA-related processes.The findings provide a basis for further research on the role of igf2bp in regulating the growth and development of the razor clam.展开更多
基金This work was supported by the grants from the National Natural Science Foundation of China[31472278]the Shanghai Universities Knowledge Service Platform[ZF1206].
文摘Insulin-like growth factor-II mRNA binding protein(igf2bp)is an mRNA binding protein that regulates the post-transcriptional fate of multiple transcripts.In this study,a novel igf2bp gene was identified in the razor clam Sinonovacula constricta.The complete 2254 bp Sc-igf2bp cDNA consists of an open reading frame(ORF)encoding 610 amino acid(aa)residues.The deduced protein includes a single RNA recognition motif(RRM)and four K homology(KH)domains.Sc-igf2bp transcripts were expressed widely during different developmental stages and in adult tissues,and a 95 kDa recombinant pEGX-4T-1-Scigf2bp protein was expressed in Escherichia coli Rosetta(DE3)pLysS cells and purified using Glutathione Sepharose 4B resin.Activity was evaluated by GST pull-down assays,and mass spectrometry results indicated that Sc-igf2bp may participate in RNA-related processes.The findings provide a basis for further research on the role of igf2bp in regulating the growth and development of the razor clam.
