获得性骨髓造血衰竭综合征的临床诊断及鉴别诊断是比较难以准确把握的临床问题，相当一部分患者的临床特征不典型，与目前再生障碍性贫血（AA）、骨髓增生异常综合征（MDS）的诊断标准不符。Wimazal等[1]及Valent等[2]提出意义未明特发...获得性骨髓造血衰竭综合征的临床诊断及鉴别诊断是比较难以准确把握的临床问题，相当一部分患者的临床特征不典型，与目前再生障碍性贫血（AA）、骨髓增生异常综合征（MDS）的诊断标准不符。Wimazal等[1]及Valent等[2]提出意义未明特发性血细胞减少症（Idiopathic cytopenia of undetermined significance，ICUS）来表述一组尚不能明确诊断的骨髓造血衰竭。近些年来，随着二代测序技术的发展，我们发现不仅80%~90%的MDS患者存在至少一种克隆性突变[3]，AA患者也可以伴有克隆性造血标志[4,5]，约35%的ICUS患者也有克隆性突变[6]，并且健康人随着年龄增大也会出现髓系肿瘤基因突变[7,8,9]。对于健康人但伴有克隆性突变者，界定为不确定潜能的克隆性造血（Clonal hematopoiesis of indeterminate potential，CHIP）[10]；对于伴有克隆性突变的ICUS患者，界定为意义未明克隆性血细胞减少症（Clonal cytopenias of undetermined significance，CCUS）[6,10]。展开更多
目的:初步分析不同年龄组冠心病心绞痛患者的冠状动脉内超声(ICUS)影像特点。方法:280例心绞痛患者按年龄分为老年(≥65岁)组92例,非老年(<65岁)组188例,应用ICUS对两组的280处缺血相关病变进行分析。结果:共分析280处病变,老年组和...目的:初步分析不同年龄组冠心病心绞痛患者的冠状动脉内超声(ICUS)影像特点。方法:280例心绞痛患者按年龄分为老年(≥65岁)组92例,非老年(<65岁)组188例,应用ICUS对两组的280处缺血相关病变进行分析。结果:共分析280处病变,老年组和非老年组的最小外弹力膜横截面积、斑块负荷、纤维帽厚度、脂质池面积、脂质池面积/斑块面积比值、重构指数分别为(13.9±3.9)mm^2 vs(15.6±4.6)mm^2、(73.4±12.8)％ vs (68.6±11.6)％、(1.12±0.76)mm vs(0.50±0.34)mm、(1.5±0.81)mm^2 vs (3.9±2.1)mm^2、(10.8±7.4)％ vs (25.0±13.2)％、(1.053±1.271) vs (1.186±1.285),P均<0.05。结论:老年冠心病的缺血相关病变以稳定性斑块为主,表现为纤维帽较厚、脂核较小、血管负重构和无重构的比例高。展开更多
Objective:To identify three common genes(bla_(TEM),bla_(SHV)and bla_(CTX-M)responsible for ESBL production in Klebsiella pneumnniae(K.pneumnniae)isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Method...Objective:To identify three common genes(bla_(TEM),bla_(SHV)and bla_(CTX-M)responsible for ESBL production in Klebsiella pneumnniae(K.pneumnniae)isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Methods:A total of 60 non-repetitive nosocomial isolates from 60patients were selected during 2009-2010.The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test(DDST)according to CLSI guidelines.The ESBL's genotype was then analyzed by multiplex PCR of bla_(TEM),bla_(SHV)and bla_(CTX-M)genes and DNA sequencing.Results:The primary susceptibility tests of K.pneumoniae showed that among 10 examined antibiotics,the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem,respectively.The phenotypic determination of ESBL by DDST showed that 60%(n=36)of isolates produced ESBL.Multiplex PCR of genes among K.pneuunoniae isolates showed that 39%(n=18)of them have TEM,39%in=18)of then have both CTX-M and TEM and 13%(n=8)of them have TEM,SHV,CTX-M.Conclusions:Our findings reveal the high prevalence(60%)of ESBL prcxducing K.pneumnniae from ICL patients along with a new pattern of bla_(TEM)distribution differ from other countries.展开更多
Numerous types of research on healthcare monitoring systems have been conducted for calculating heart rate,ECG,nasal/oral airflow,temperature,light sensor,and fall detection sensor.Different researchers have done diff...Numerous types of research on healthcare monitoring systems have been conducted for calculating heart rate,ECG,nasal/oral airflow,temperature,light sensor,and fall detection sensor.Different researchers have done different work in the field of health monitoring with sensor networks.Different researchers used built-in apps,such as some used a small number of parameters,while some other studies used more than one microcontroller and used senders and receivers among the microcontrollers to communicate,and outdated tools for study development.While no efficient,cheap,and updated work is proposed in the field of sensor-based health monitoring systems.Therefore,this study developed an android-based mobile system that can remotely monitor electrocardiograms(ECGs),pulse oximetry,heart rate,and body temperature.The microcontroller’s Wi-Fi device is used to manage wireless data transport.The findings of the patient are saved on the Firebase server for further usage in the mobile app.The performance of the proposed device is tested on ten numbers of different patients age-wise in terms of beats per minute(BPM),ECG,Temperature,and SpO2.This system uses temperature,pulse,ECG,blood pressure,and eye blink sensors.This device makes the usage of a tiny pulse sensor that has been designed to provide an accurate and optimal readout of the pulse rate and a temperature sensor is also included.With the help of an MCU,our system measures the pulse rate in beats per minute(bpm),blood oxygen level temperature measurements,and ECG readings and communicates this information to the Firebase server.To check the performance of the proposed system first,the BPM parameter was checked on the cardiac monitor.Then,the proposed model is tested on different patients age-wise.The simulation result shows that the BPM reading is not much different than the BPM of the cardiac monitor.According to the simulation findings,the proposed model achieved the best performance as compared to commercially available devices.展开更多
AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： I...AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.展开更多
文摘获得性骨髓造血衰竭综合征的临床诊断及鉴别诊断是比较难以准确把握的临床问题，相当一部分患者的临床特征不典型，与目前再生障碍性贫血（AA）、骨髓增生异常综合征（MDS）的诊断标准不符。Wimazal等[1]及Valent等[2]提出意义未明特发性血细胞减少症（Idiopathic cytopenia of undetermined significance，ICUS）来表述一组尚不能明确诊断的骨髓造血衰竭。近些年来，随着二代测序技术的发展，我们发现不仅80%~90%的MDS患者存在至少一种克隆性突变[3]，AA患者也可以伴有克隆性造血标志[4,5]，约35%的ICUS患者也有克隆性突变[6]，并且健康人随着年龄增大也会出现髓系肿瘤基因突变[7,8,9]。对于健康人但伴有克隆性突变者，界定为不确定潜能的克隆性造血（Clonal hematopoiesis of indeterminate potential，CHIP）[10]；对于伴有克隆性突变的ICUS患者，界定为意义未明克隆性血细胞减少症（Clonal cytopenias of undetermined significance，CCUS）[6,10]。
文摘目的:初步分析不同年龄组冠心病心绞痛患者的冠状动脉内超声(ICUS)影像特点。方法:280例心绞痛患者按年龄分为老年(≥65岁)组92例,非老年(<65岁)组188例,应用ICUS对两组的280处缺血相关病变进行分析。结果:共分析280处病变,老年组和非老年组的最小外弹力膜横截面积、斑块负荷、纤维帽厚度、脂质池面积、脂质池面积/斑块面积比值、重构指数分别为(13.9±3.9)mm^2 vs(15.6±4.6)mm^2、(73.4±12.8)％ vs (68.6±11.6)％、(1.12±0.76)mm vs(0.50±0.34)mm、(1.5±0.81)mm^2 vs (3.9±2.1)mm^2、(10.8±7.4)％ vs (25.0±13.2)％、(1.053±1.271) vs (1.186±1.285),P均<0.05。结论:老年冠心病的缺血相关病变以稳定性斑块为主,表现为纤维帽较厚、脂核较小、血管负重构和无重构的比例高。
基金supported by Jinan Science and Technology Deyelopment Plans Grant(No.201121040)
文摘Objective:To identify three common genes(bla_(TEM),bla_(SHV)and bla_(CTX-M)responsible for ESBL production in Klebsiella pneumnniae(K.pneumnniae)isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Methods:A total of 60 non-repetitive nosocomial isolates from 60patients were selected during 2009-2010.The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test(DDST)according to CLSI guidelines.The ESBL's genotype was then analyzed by multiplex PCR of bla_(TEM),bla_(SHV)and bla_(CTX-M)genes and DNA sequencing.Results:The primary susceptibility tests of K.pneumoniae showed that among 10 examined antibiotics,the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem,respectively.The phenotypic determination of ESBL by DDST showed that 60%(n=36)of isolates produced ESBL.Multiplex PCR of genes among K.pneuunoniae isolates showed that 39%(n=18)of them have TEM,39%in=18)of then have both CTX-M and TEM and 13%(n=8)of them have TEM,SHV,CTX-M.Conclusions:Our findings reveal the high prevalence(60%)of ESBL prcxducing K.pneumnniae from ICL patients along with a new pattern of bla_(TEM)distribution differ from other countries.
基金Deputyship for Research&Innovation,Ministry of Education in Saudi Arabia for funding this research work through the Project Number 223202.
文摘Numerous types of research on healthcare monitoring systems have been conducted for calculating heart rate,ECG,nasal/oral airflow,temperature,light sensor,and fall detection sensor.Different researchers have done different work in the field of health monitoring with sensor networks.Different researchers used built-in apps,such as some used a small number of parameters,while some other studies used more than one microcontroller and used senders and receivers among the microcontrollers to communicate,and outdated tools for study development.While no efficient,cheap,and updated work is proposed in the field of sensor-based health monitoring systems.Therefore,this study developed an android-based mobile system that can remotely monitor electrocardiograms(ECGs),pulse oximetry,heart rate,and body temperature.The microcontroller’s Wi-Fi device is used to manage wireless data transport.The findings of the patient are saved on the Firebase server for further usage in the mobile app.The performance of the proposed device is tested on ten numbers of different patients age-wise in terms of beats per minute(BPM),ECG,Temperature,and SpO2.This system uses temperature,pulse,ECG,blood pressure,and eye blink sensors.This device makes the usage of a tiny pulse sensor that has been designed to provide an accurate and optimal readout of the pulse rate and a temperature sensor is also included.With the help of an MCU,our system measures the pulse rate in beats per minute(bpm),blood oxygen level temperature measurements,and ECG readings and communicates this information to the Firebase server.To check the performance of the proposed system first,the BPM parameter was checked on the cardiac monitor.Then,the proposed model is tested on different patients age-wise.The simulation result shows that the BPM reading is not much different than the BPM of the cardiac monitor.According to the simulation findings,the proposed model achieved the best performance as compared to commercially available devices.
文摘AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.
