Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard D...Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.展开更多
立足信息及通信融合的视角,提出基于业务承载的配网通信网信息通信技术(Information and Communication Technology,ICT)测试平台设计思路,并从测试功能、业务源模型两个方面阐述了此测试平台的框架设计。首先分析了基于业务承载的配网...立足信息及通信融合的视角,提出基于业务承载的配网通信网信息通信技术(Information and Communication Technology,ICT)测试平台设计思路,并从测试功能、业务源模型两个方面阐述了此测试平台的框架设计。首先分析了基于业务承载的配网通信网ICT测试平台实例设计,其次介绍了此测试软件的测试平台功能模块与执行流程,最后通过在无源光纤网络(Passive Optical Network,PON)与可编程逻辑控制器(Programmable Logic Controller,PLC)混合组网方案中的实际应用,得出了通信性能和业务承载性能两方面的测试分析结果,验证了此测试平台在配网通信网工程中的实用性。展开更多
文摘Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.
基金拉里奥哈国际大学通过工程与技术学院(School for Engineering ＆ Technolo-gy)(http：//esit.unir.net)和教育创新和技术研究院(Research Institute for Innovation ＆ Technology in Education,简称iTED,http：//research.unir.net/it-ed)提供的部分资助
文摘立足信息及通信融合的视角,提出基于业务承载的配网通信网信息通信技术(Information and Communication Technology,ICT)测试平台设计思路,并从测试功能、业务源模型两个方面阐述了此测试平台的框架设计。首先分析了基于业务承载的配网通信网ICT测试平台实例设计,其次介绍了此测试软件的测试平台功能模块与执行流程,最后通过在无源光纤网络(Passive Optical Network,PON)与可编程逻辑控制器(Programmable Logic Controller,PLC)混合组网方案中的实际应用,得出了通信性能和业务承载性能两方面的测试分析结果,验证了此测试平台在配网通信网工程中的实用性。
