目的对足月新生儿缺氧缺血性脑病(HIE)生后6 h 内振幅整合脑电图(aEEG)的变化及其在 HIE 早期诊断和神经学预后评估的价值进行初步探讨。方法对2003年5月至2005年2月间在我院新生儿病房住院的33例足月 HIE 患儿在生后6 h 内进行 aEEG 描...目的对足月新生儿缺氧缺血性脑病(HIE)生后6 h 内振幅整合脑电图(aEEG)的变化及其在 HIE 早期诊断和神经学预后评估的价值进行初步探讨。方法对2003年5月至2005年2月间在我院新生儿病房住院的33例足月 HIE 患儿在生后6 h 内进行 aEEG 描记,并将 aEEG 监测结果与 HIE 患儿临床分度及18个月时的神经学预后进行相关性分析,分析其在 HIE 早期诊断和神经学预后预测中的价值。结果 33例 HIE 患儿中,aEEG 正常20例(60.6%),轻度异常5例(15.2%),重度异常8例(24.2%)。33例 HIE 患儿中,轻度 HIE 17例(51.5%),中度 HIE 9例(27.3%),重度HIE 7例(21.2%)。25例进行神经预后分析,其中19例神经学预后正常,1例伤残(智力缺陷),5例死亡。aEEG 分类结果与 HIE 临床分度及其神经学预后均相关性强。aEEG 异常预测新生儿中重度HIE 的敏感性为100%,特异性为81.3%,阳性预测值为85.0%,阴性预测值为100%;预测 HIE 异常神经学预后的敏感性为100%、特异性为90.9%、阳性预测值为93.3%和阴性预测值为100%。结论对足月 HIE 新生儿生后6 h 内 aEEG 监测能早期预测 HIE 病情轻重程度并预测其神经学预后。展开更多
Objective: To explore cell death and apoptosis in rat hippocampal neurons at different time points after ischemia, hypoxia and reperfusion injury and to elucidate time window characteristics in ischemia neuronal injur...Objective: To explore cell death and apoptosis in rat hippocampal neurons at different time points after ischemia, hypoxia and reperfusion injury and to elucidate time window characteristics in ischemia neuronal injury. Methods: Hippocampal neurons were obtained from rat embryo and were cultured in vitro. The ischemia and reperfusion of cultured rat hippocampal neurons were simulated by oxygen-glucose deprivation (OGD) and recovery. OGD at different time points ((0.25) h to (3.0) h) and then the same recovery (24 h) were prepared. Annexin (V-PI) staining and flow cytometry examined neuron death and apoptosis at different time after injury. Results: After OGD and recovery, both necrosis and apoptosis were observed. At different times after OGD, there were statistically significant differences in neuron necrosis rate (P<(0.05)), but not in apoptosis rate (P>(0.05)). At recovery, survival rate of hippocampal neurons further decreased while apoptosis rate increased. Furthermore, apoptosis rates of different time differed greatly (P<(0.05)). Apoptosis rate gradually increased with significant difference among those of different time points (P<(0.05)). However, 2 h after ischemia, apoptosis rate decreased markedly. Conclusions: Apoptosis is an important pathway of delayed neuron death. The therapeutic time window should be within 2 h after cerebral ischemia and hypoxia.展开更多
Perinatal hypoxic-ischemic encephalopathy is a leading cause of neonatal death and disability.Therapeutic hypothermia significantly reduces death and major disability associated with hypoxic-ischemic encephalopathy;ho...Perinatal hypoxic-ischemic encephalopathy is a leading cause of neonatal death and disability.Therapeutic hypothermia significantly reduces death and major disability associated with hypoxic-ischemic encephalopathy;however,many infants still experience lifelong disabilities to movement,sensation and cognition.Clinical guidelines,based on strong clinical and preclinical evidence,recommend therapeutic hypothermia should be started within 6 hours of birth and continued for a period of 72 hours,with a target brain temperature of 33.5 ±0.5℃ for infants with moderate to severe hypoxic-ischemic encephalopathy.The clinical guidelines also recommend that infants be re warmed at a rate of 0.5℃ per hour,but this is not based on strong evidence.There are no randomized controlled trials investigating the optimal rate of rewarming after therapeutic hypothermia for infants with hypoxic-ischemic encephalopathy.Preclinical studies of rewarming are conflicting and results were confounded by treatment with sub-optimal durations of hypothermia.In this review,we evaluate the evidence for the optimal start time,duration and depth of hypothermia,and whether the rate of rewarming after treatment affects brain injury and neurological outcomes.展开更多
文摘目的对足月新生儿缺氧缺血性脑病(HIE)生后6 h 内振幅整合脑电图(aEEG)的变化及其在 HIE 早期诊断和神经学预后评估的价值进行初步探讨。方法对2003年5月至2005年2月间在我院新生儿病房住院的33例足月 HIE 患儿在生后6 h 内进行 aEEG 描记,并将 aEEG 监测结果与 HIE 患儿临床分度及18个月时的神经学预后进行相关性分析,分析其在 HIE 早期诊断和神经学预后预测中的价值。结果 33例 HIE 患儿中,aEEG 正常20例(60.6%),轻度异常5例(15.2%),重度异常8例(24.2%)。33例 HIE 患儿中,轻度 HIE 17例(51.5%),中度 HIE 9例(27.3%),重度HIE 7例(21.2%)。25例进行神经预后分析,其中19例神经学预后正常,1例伤残(智力缺陷),5例死亡。aEEG 分类结果与 HIE 临床分度及其神经学预后均相关性强。aEEG 异常预测新生儿中重度HIE 的敏感性为100%,特异性为81.3%,阳性预测值为85.0%,阴性预测值为100%;预测 HIE 异常神经学预后的敏感性为100%、特异性为90.9%、阳性预测值为93.3%和阴性预测值为100%。结论对足月 HIE 新生儿生后6 h 内 aEEG 监测能早期预测 HIE 病情轻重程度并预测其神经学预后。
文摘Objective: To explore cell death and apoptosis in rat hippocampal neurons at different time points after ischemia, hypoxia and reperfusion injury and to elucidate time window characteristics in ischemia neuronal injury. Methods: Hippocampal neurons were obtained from rat embryo and were cultured in vitro. The ischemia and reperfusion of cultured rat hippocampal neurons were simulated by oxygen-glucose deprivation (OGD) and recovery. OGD at different time points ((0.25) h to (3.0) h) and then the same recovery (24 h) were prepared. Annexin (V-PI) staining and flow cytometry examined neuron death and apoptosis at different time after injury. Results: After OGD and recovery, both necrosis and apoptosis were observed. At different times after OGD, there were statistically significant differences in neuron necrosis rate (P<(0.05)), but not in apoptosis rate (P>(0.05)). At recovery, survival rate of hippocampal neurons further decreased while apoptosis rate increased. Furthermore, apoptosis rates of different time differed greatly (P<(0.05)). Apoptosis rate gradually increased with significant difference among those of different time points (P<(0.05)). However, 2 h after ischemia, apoptosis rate decreased markedly. Conclusions: Apoptosis is an important pathway of delayed neuron death. The therapeutic time window should be within 2 h after cerebral ischemia and hypoxia.
基金supported by The Health Research Council of New Zealand(grant No.16/003,17/601)the Marsden Fund(grant No.17-UOA232)a Sir Charles Hercus Fellowship from the Health Research Council of New Zealand(grant No.16/003)
文摘Perinatal hypoxic-ischemic encephalopathy is a leading cause of neonatal death and disability.Therapeutic hypothermia significantly reduces death and major disability associated with hypoxic-ischemic encephalopathy;however,many infants still experience lifelong disabilities to movement,sensation and cognition.Clinical guidelines,based on strong clinical and preclinical evidence,recommend therapeutic hypothermia should be started within 6 hours of birth and continued for a period of 72 hours,with a target brain temperature of 33.5 ±0.5℃ for infants with moderate to severe hypoxic-ischemic encephalopathy.The clinical guidelines also recommend that infants be re warmed at a rate of 0.5℃ per hour,but this is not based on strong evidence.There are no randomized controlled trials investigating the optimal rate of rewarming after therapeutic hypothermia for infants with hypoxic-ischemic encephalopathy.Preclinical studies of rewarming are conflicting and results were confounded by treatment with sub-optimal durations of hypothermia.In this review,we evaluate the evidence for the optimal start time,duration and depth of hypothermia,and whether the rate of rewarming after treatment affects brain injury and neurological outcomes.
