1例41岁女性患者因腹部隐痛行盆腔增强CT检查。在医生给予其碘佛醇注射液1 m L做过敏试验后40 min,患者出现憋气、恶心、呕吐、面色苍白,四肢发凉,血压测不到。即刻静脉注射地塞米松磷酸钠注射液、肾上腺素注射液,并数次静脉使用注射用...1例41岁女性患者因腹部隐痛行盆腔增强CT检查。在医生给予其碘佛醇注射液1 m L做过敏试验后40 min,患者出现憋气、恶心、呕吐、面色苍白,四肢发凉,血压测不到。即刻静脉注射地塞米松磷酸钠注射液、肾上腺素注射液,并数次静脉使用注射用甲泼尼龙琥珀酸钠、多巴胺注射液、呋塞米注射液、氯化钠注射液等药物对症治疗。12 h后患者各项生命体征平稳。展开更多
Three crosses,Hawaii7981×PI128216,Hawaii7981×LA1589,and PI128216×LA1589,were made to develop F_2 populations for testing allelism among three genes Xv3,Rx4,and Rx_(LA1589) conferring resistance to bacte...Three crosses,Hawaii7981×PI128216,Hawaii7981×LA1589,and PI128216×LA1589,were made to develop F_2 populations for testing allelism among three genes Xv3,Rx4,and Rx_(LA1589) conferring resistance to bacterial spot caused by Xanthomonas perforans race T3 in tomato. Each population consisted of 535–1 655 individuals. An infiltration method was used to inoculate the leaves of the parental and F_2 plants as well as the susceptible control OH88119 for detecting hypersensitive resistance(HR). The results showed that all the tomato plants except OH88119 had HR to race T3,indicating that Xv3,Rx4,and Rx_(LA1589) were allelic genes. Genomic DNA fragments of the Rx4 alleles from Hawaii7981,PI128216,and LA1589 were amplified using gene-specific primers and sequenced. No sequence variation was observed in the coding region of Rx4 in the three resistant lines. Based on the published map positions of these loci as well as the allelic tests and sequence data obtained in this study,we speculated that Xv3,Rx4,and Rx_(LA1589) were the same gene. The results will provide useful information for understanding the mechanism of resistance to race T3 and developing resistant tomato varieties.展开更多
文摘1例41岁女性患者因腹部隐痛行盆腔增强CT检查。在医生给予其碘佛醇注射液1 m L做过敏试验后40 min,患者出现憋气、恶心、呕吐、面色苍白,四肢发凉,血压测不到。即刻静脉注射地塞米松磷酸钠注射液、肾上腺素注射液,并数次静脉使用注射用甲泼尼龙琥珀酸钠、多巴胺注射液、呋塞米注射液、氯化钠注射液等药物对症治疗。12 h后患者各项生命体征平稳。
基金supported by the National Natural Science Foundation of China(Grant No.31372073)Beijing Modern Agro-industry Technology Research System(Grant No.GCTDZJ2014033001)
文摘Three crosses,Hawaii7981×PI128216,Hawaii7981×LA1589,and PI128216×LA1589,were made to develop F_2 populations for testing allelism among three genes Xv3,Rx4,and Rx_(LA1589) conferring resistance to bacterial spot caused by Xanthomonas perforans race T3 in tomato. Each population consisted of 535–1 655 individuals. An infiltration method was used to inoculate the leaves of the parental and F_2 plants as well as the susceptible control OH88119 for detecting hypersensitive resistance(HR). The results showed that all the tomato plants except OH88119 had HR to race T3,indicating that Xv3,Rx4,and Rx_(LA1589) were allelic genes. Genomic DNA fragments of the Rx4 alleles from Hawaii7981,PI128216,and LA1589 were amplified using gene-specific primers and sequenced. No sequence variation was observed in the coding region of Rx4 in the three resistant lines. Based on the published map positions of these loci as well as the allelic tests and sequence data obtained in this study,we speculated that Xv3,Rx4,and Rx_(LA1589) were the same gene. The results will provide useful information for understanding the mechanism of resistance to race T3 and developing resistant tomato varieties.
