Objective: To observe the blocking effect of Huoxiang Zhengqi liqu id (HXZQL) on histamine release of basophils. Methods: The study was conducted using HXZQL to neutralize and b lock IgE and antagonize the effect of I...Objective: To observe the blocking effect of Huoxiang Zhengqi liqu id (HXZQL) on histamine release of basophils. Methods: The study was conducted using HXZQL to neutralize and b lock IgE and antagonize the effect of IL 3 induced enhancing histamine relea se of bosaphils. Concentration and percentage of histamine release of bosaphils were tested by the basophil histamine release test. Results: The HXZQL drug serum had obvious inhibitive effect on degranulation of bosaphils induced by IgE antigen antibody complex ( P <0.0 5) and can successfully weaken the histamine release of basophils stimulated by IL 3. When compared with those in the untreated group, it showed P <0.01. Conclusion: HXZQL drug serum may neutralize and block IgE and has inhibitive effects on degranulation of cells when resensitized by antigens. It can also block allergic response type Ⅰ by antagonizing the effect of IL 3 induced enhancing histamine release.展开更多
To establish HPLC characteristic spectrum of Huoxiang(Herba Agastachis)Eliminating Summer-heat Soft Capsule.Chromatographic separation was performed on Tech Mate C18-ST(5 um,4.6×250 nm)column by Agilent 1260 high...To establish HPLC characteristic spectrum of Huoxiang(Herba Agastachis)Eliminating Summer-heat Soft Capsule.Chromatographic separation was performed on Tech Mate C18-ST(5 um,4.6×250 nm)column by Agilent 1260 high performance liquid chromatograph,eluted with acetonitrile and 0.05%phosphoric acid in a gradient elution at a flow rate of 1.0 mL∙min-1.The detection wavelength was set at 270 nm.Hesperidin was selected as the reference peak to calculate the RSD of the relative retention time.Precision,stability and reproducibility were investigated as well.80 different samples from 5 manufacturers were included in this study.The HPLC characteristic spectrum of Huoxiang Eliminating Summer-heat Soft Capsule was constructed with 11 specific chromatographic peaks in 80 samples.The method is accurate,reliable and with good reproducibility,and could overall control the quality of Huoxiang Eliminating Summerheat Soft Capsule.展开更多
基金The item is subsidized by Natural Science Fund of Fujian Province (No. C96044)
文摘Objective: To observe the blocking effect of Huoxiang Zhengqi liqu id (HXZQL) on histamine release of basophils. Methods: The study was conducted using HXZQL to neutralize and b lock IgE and antagonize the effect of IL 3 induced enhancing histamine relea se of bosaphils. Concentration and percentage of histamine release of bosaphils were tested by the basophil histamine release test. Results: The HXZQL drug serum had obvious inhibitive effect on degranulation of bosaphils induced by IgE antigen antibody complex ( P <0.0 5) and can successfully weaken the histamine release of basophils stimulated by IL 3. When compared with those in the untreated group, it showed P <0.01. Conclusion: HXZQL drug serum may neutralize and block IgE and has inhibitive effects on degranulation of cells when resensitized by antigens. It can also block allergic response type Ⅰ by antagonizing the effect of IL 3 induced enhancing histamine release.
基金This work is supported by National Comprehensive Drug Evaluation Fund Projects of National Food and Drug Administration in 2016.
文摘To establish HPLC characteristic spectrum of Huoxiang(Herba Agastachis)Eliminating Summer-heat Soft Capsule.Chromatographic separation was performed on Tech Mate C18-ST(5 um,4.6×250 nm)column by Agilent 1260 high performance liquid chromatograph,eluted with acetonitrile and 0.05%phosphoric acid in a gradient elution at a flow rate of 1.0 mL∙min-1.The detection wavelength was set at 270 nm.Hesperidin was selected as the reference peak to calculate the RSD of the relative retention time.Precision,stability and reproducibility were investigated as well.80 different samples from 5 manufacturers were included in this study.The HPLC characteristic spectrum of Huoxiang Eliminating Summer-heat Soft Capsule was constructed with 11 specific chromatographic peaks in 80 samples.The method is accurate,reliable and with good reproducibility,and could overall control the quality of Huoxiang Eliminating Summerheat Soft Capsule.
