The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro,as well as the roles of nuclear factor-kappa B（NF-κB） and IκBα was investigated.The effect of tritptolide...The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro,as well as the roles of nuclear factor-kappa B（NF-κB） and IκBα was investigated.The effect of tritptolide on the growth of RPMI-8226 cells was studied by MTT assay.Apoptosis was detected by Hoechest 33258 staining and Annexin V/PI double staining assay.The expression of NF-κB and IκBα was observed by Western blot and confocal microscopy.The results showed that triptolide inactivated NF-κB apoptotic pathway in human multiple myeloma RPMI-8226 cells.Triptolide at nM range induced proliferation inhibition in a dose-and time-dependent manner and apoptosis in a dose-dependent fashion in RPMI-8226 cells.Besides,we observed the inhibition of NF-κB /p65 in the nuclear fraction was correlated with the increase in the protein expression of IκBα in the cytosol.These results suggested that triptolide might exhibit its strong anti-tumor effects via inactivation of NF-κB/p65 and IκBα.展开更多
目的探讨重组人促红细胞生成素(r HuEPO)治疗多发性骨髓瘤小鼠的疗效及其可能作用机制。方法 420只BALB/c小鼠,荷瘤组410只和正常对照组10只。荷瘤组小鼠均皮下接种1×104个MPC-11骨髓瘤细胞,荷瘤后第5 d随机抽取50只作为荷瘤对照组...目的探讨重组人促红细胞生成素(r HuEPO)治疗多发性骨髓瘤小鼠的疗效及其可能作用机制。方法 420只BALB/c小鼠,荷瘤组410只和正常对照组10只。荷瘤组小鼠均皮下接种1×104个MPC-11骨髓瘤细胞,荷瘤后第5 d随机抽取50只作为荷瘤对照组,余小鼠随机分为3个不同剂量的r HuEPO(10 I U、20 I U、30 I U)治疗组,120只/组。r HuEPO皮下注射,每天1次,连续注射2周后减为1周3次。荷瘤对照组小鼠接受生理盐水皮下注射。荷瘤后动态监测血清M蛋白的出现时间,检测小鼠血红蛋白(Hb)水平,并取皮下结节进行病理检查。测定血清IL-6、TNF-α、TNF-γ、IgG、κ轻链浓度,全血CD4+、CD8+细胞计数(流式细胞分析方法),肿瘤组织的微血管密度(MVD)以及肿瘤细胞凋亡(TUNEL法)。结果荷瘤后22 d检测到荷瘤小鼠血清中出现M蛋白;r HuEPO治疗后,各治疗组的Hb水平及生存时间均高于荷瘤对照组(P<0.05),各治疗组间差异无统计学意义(P>0.05);r HuEPO应用2月后,各治疗组血清IL-6、IgG及κ轻链的浓度均低于荷瘤对照组(P<0.05);荷瘤小鼠的总体生存时间与Hb水平呈正相关(P=0.000)、与血清IL-6水平呈负相关(P=0.009)。结论 r HuEPO明显提高荷瘤小鼠的Hb水平并延长其生存时间,同时降低其血清IL-6水平和M蛋白水平。展开更多
基金supported by a grant from National Natural Sciences Foundation of China (No. 30700882)
文摘The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro,as well as the roles of nuclear factor-kappa B（NF-κB） and IκBα was investigated.The effect of tritptolide on the growth of RPMI-8226 cells was studied by MTT assay.Apoptosis was detected by Hoechest 33258 staining and Annexin V/PI double staining assay.The expression of NF-κB and IκBα was observed by Western blot and confocal microscopy.The results showed that triptolide inactivated NF-κB apoptotic pathway in human multiple myeloma RPMI-8226 cells.Triptolide at nM range induced proliferation inhibition in a dose-and time-dependent manner and apoptosis in a dose-dependent fashion in RPMI-8226 cells.Besides,we observed the inhibition of NF-κB /p65 in the nuclear fraction was correlated with the increase in the protein expression of IκBα in the cytosol.These results suggested that triptolide might exhibit its strong anti-tumor effects via inactivation of NF-κB/p65 and IκBα.
文摘目的探讨重组人促红细胞生成素(r HuEPO)治疗多发性骨髓瘤小鼠的疗效及其可能作用机制。方法 420只BALB/c小鼠,荷瘤组410只和正常对照组10只。荷瘤组小鼠均皮下接种1×104个MPC-11骨髓瘤细胞,荷瘤后第5 d随机抽取50只作为荷瘤对照组,余小鼠随机分为3个不同剂量的r HuEPO(10 I U、20 I U、30 I U)治疗组,120只/组。r HuEPO皮下注射,每天1次,连续注射2周后减为1周3次。荷瘤对照组小鼠接受生理盐水皮下注射。荷瘤后动态监测血清M蛋白的出现时间,检测小鼠血红蛋白(Hb)水平,并取皮下结节进行病理检查。测定血清IL-6、TNF-α、TNF-γ、IgG、κ轻链浓度,全血CD4+、CD8+细胞计数(流式细胞分析方法),肿瘤组织的微血管密度(MVD)以及肿瘤细胞凋亡(TUNEL法)。结果荷瘤后22 d检测到荷瘤小鼠血清中出现M蛋白;r HuEPO治疗后,各治疗组的Hb水平及生存时间均高于荷瘤对照组(P<0.05),各治疗组间差异无统计学意义(P>0.05);r HuEPO应用2月后,各治疗组血清IL-6、IgG及κ轻链的浓度均低于荷瘤对照组(P<0.05);荷瘤小鼠的总体生存时间与Hb水平呈正相关(P=0.000)、与血清IL-6水平呈负相关(P=0.009)。结论 r HuEPO明显提高荷瘤小鼠的Hb水平并延长其生存时间,同时降低其血清IL-6水平和M蛋白水平。
