目的:观察局部联合注射外源性人胰岛素样生长因子1(human insulin like growth factor1,hIGF1)和人γ干扰素(human interferonγ,hIFNγ)对于小鼠急性钝挫伤骨骼肌修复过程中再生和纤维化的影响。方法:64只8周龄雄性C3H小鼠,制作右侧腓...目的:观察局部联合注射外源性人胰岛素样生长因子1(human insulin like growth factor1,hIGF1)和人γ干扰素(human interferonγ,hIFNγ)对于小鼠急性钝挫伤骨骼肌修复过程中再生和纤维化的影响。方法:64只8周龄雄性C3H小鼠,制作右侧腓肠肌钝挫伤动物模型,随机分为4组,即A组(注射hIFNγ)、B组(注射hIGF1)、C组(联合注射hIFNγ和hIGF1)、D组(注射生理盐水)。挫伤后第10天,A、B、C、D组小鼠,腓肠肌损伤处分别注射不同药物进行干预。于干预前(伤后7d),和干预后4、18、32d,各组随机抽取4只小鼠进行损伤腓肠肌取材,以荧光定量PCR和免疫荧光化学染色方法检测不同时点Ⅱb型肌球蛋白重链(myosin heavy chainⅡb,MHCⅡb)及波形蛋白(Vimentin)的表达。结果:①干预后各时点,B、C组小鼠损伤骨骼肌局部MHCⅡb表达较A、D组明显高;②干预后各时间点A、B、C组小鼠损伤骨骼肌局部Vimentin表达较D组低,其中以A组和B组更明显。结论:①骨骼肌急性损伤后,局部注射hIGF1有明显促进骨骼肌纤维再生,和一定程度抑制纤维化的作用。②局部注射hIFNγ仅表现出抑制纤维化的作用,且比hIGF1更加明显。③联合应用hIGF1和hIFNγ,能够同时促进骨骼肌再生和抑制纤维化,有效地促进损伤骨骼肌的愈合。展开更多
Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cells were transplanted into a hypoxic-ischemic neonatal rat model via the tail vein. BrdU-positive cells at day 7 post-transplantation, ...Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cells were transplanted into a hypoxic-ischemic neonatal rat model via the tail vein. BrdU-positive cells at day 7 post-transplantation, as well as nestin- and neuron specific enolase-positive cells at day 14 were increased compared with those of the single neural stem cell transplantation group. In addition, the proportion of neuronal differentiation was enhanced. The genetically modified cell-transplanted rats exhibited enhanced performance in correctly crossing a Y-maze and climbing an angled slope compared with those of the single neural stem cell transplantation group. These results showed that human insulin-like growth factor 1-transfected neural stem cell transplantation promotes the recovery of the leaming, memory and motor functions in hypoxic-ischemic rats.展开更多
文摘目的:观察局部联合注射外源性人胰岛素样生长因子1(human insulin like growth factor1,hIGF1)和人γ干扰素(human interferonγ,hIFNγ)对于小鼠急性钝挫伤骨骼肌修复过程中再生和纤维化的影响。方法:64只8周龄雄性C3H小鼠,制作右侧腓肠肌钝挫伤动物模型,随机分为4组,即A组(注射hIFNγ)、B组(注射hIGF1)、C组(联合注射hIFNγ和hIGF1)、D组(注射生理盐水)。挫伤后第10天,A、B、C、D组小鼠,腓肠肌损伤处分别注射不同药物进行干预。于干预前(伤后7d),和干预后4、18、32d,各组随机抽取4只小鼠进行损伤腓肠肌取材,以荧光定量PCR和免疫荧光化学染色方法检测不同时点Ⅱb型肌球蛋白重链(myosin heavy chainⅡb,MHCⅡb)及波形蛋白(Vimentin)的表达。结果:①干预后各时点,B、C组小鼠损伤骨骼肌局部MHCⅡb表达较A、D组明显高;②干预后各时间点A、B、C组小鼠损伤骨骼肌局部Vimentin表达较D组低,其中以A组和B组更明显。结论:①骨骼肌急性损伤后,局部注射hIGF1有明显促进骨骼肌纤维再生,和一定程度抑制纤维化的作用。②局部注射hIFNγ仅表现出抑制纤维化的作用,且比hIGF1更加明显。③联合应用hIGF1和hIFNγ,能够同时促进骨骼肌再生和抑制纤维化,有效地促进损伤骨骼肌的愈合。
基金the National Natural Science Foundation of China, No.30770758, 81071114
文摘Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cells were transplanted into a hypoxic-ischemic neonatal rat model via the tail vein. BrdU-positive cells at day 7 post-transplantation, as well as nestin- and neuron specific enolase-positive cells at day 14 were increased compared with those of the single neural stem cell transplantation group. In addition, the proportion of neuronal differentiation was enhanced. The genetically modified cell-transplanted rats exhibited enhanced performance in correctly crossing a Y-maze and climbing an angled slope compared with those of the single neural stem cell transplantation group. These results showed that human insulin-like growth factor 1-transfected neural stem cell transplantation promotes the recovery of the leaming, memory and motor functions in hypoxic-ischemic rats.
