环黄芪醇作为黄芪中一种极具药用价值的次生代谢产物,对心血管疾病、脑卒中、脂肪肝、腹主动脉瘤等疾病均有保护作用,可激活端粒酶、调节免疫力、促进伤口愈合及毛发生长等。但目前对于环黄芪醇的药理作用研究还存在一些问题:①相关体...环黄芪醇作为黄芪中一种极具药用价值的次生代谢产物,对心血管疾病、脑卒中、脂肪肝、腹主动脉瘤等疾病均有保护作用,可激活端粒酶、调节免疫力、促进伤口愈合及毛发生长等。但目前对于环黄芪醇的药理作用研究还存在一些问题:①相关体外实验发现环黄芪醇在一定浓度下具有细胞毒性作用,提示环黄芪醇并非绝对安全,其对细胞的毒性作用尚需深入研究,同时在动物体内的安全性还需进一步验证;②对于环黄芪醇的研究多局限于激活端粒酶这一作用靶点,虽使其在相关保健产品开发中存在优势,但一定程度上限制了对其更深层次和更广范围的研究,目前虽已有环黄芪醇对腺苷酸活化蛋白激酶(AMP activiated protein kinase,AMPK)通路、法尼醇X受体(famesoid X receptor,FXR)转录因子和沉默信息调节蛋白(silent mating type information regulation,SIRT1)蛋白调节作用的探讨,为其药理作用靶点做了一定的补充,但仍缺乏相关的药理活性和作用机制研究。尽管对环黄芪醇的药理作用研究还存在一些问题,但不可否认其药用价值,今后需进一步加强其对心脏纤维化、充血性心力衰竭和心律失常等疾病的保护作用研究,以及对于改善心血管疾病风险指标、调节内皮细胞稳态作用的理论和实验研究,以更好地应用于临床。同时,充分挖掘环黄芪醇在治疗脑缺血和精神类疾病方面的潜力,将其对脑部神经血管疾病的作用作为未来研究的一个新方向。展开更多
目的基于网络药理学探讨黄芪治疗类风湿关节炎合并肺间质纤维化的潜在作用机制。方法通过中药系统药理学技术平台(TCMSP)筛选出黄芪中的潜在活性成分,整合TCMSP数据库、靶点预测网站(Swiss Target Prediction)、人类孟德尔遗传数据库(OM...目的基于网络药理学探讨黄芪治疗类风湿关节炎合并肺间质纤维化的潜在作用机制。方法通过中药系统药理学技术平台(TCMSP)筛选出黄芪中的潜在活性成分,整合TCMSP数据库、靶点预测网站(Swiss Target Prediction)、人类孟德尔遗传数据库(OMIM)、GCBI文献数据库和人类基因数据库(Genecards),预测和筛选其治疗类风湿关节炎合并肺间质纤维化的作用靶点,运用韦恩图(Venny 2.1.0)对药物靶点和疾病靶点进行匹配。通过Systems Dock Web Site对主要活性成分和作用靶点进行分子对接验证。运用String数据库和Cytoscape软件构建绘制蛋白质相互作用网络,并用DisGeNET数据库对作用靶点进行类型归属。采用DAVID数据库对黄芪核心作用靶点进行生物功能和代谢通路分析,借助Cytoscape软件构建"药物-活性成分-核心靶点-通路-疾病"网络。结果筛选得到黄芪25个潜在活性成分,涉及9个作用靶点,与疾病靶点有关的活性成分有3个,主要通过调控TNF、NF-κB、MAPK、Toll样受体、TGF-β信号通路发挥治疗作用。结论黄芪对类风湿关节炎合并肺间质纤维化的治疗作用体现了中药多成分-多靶点-多途径的特点,为阐释其治疗类风湿关节炎合并肺间质纤维化的作用机制提供了科学依据。展开更多
Objective This study aimed to analyze the mechanism of action of Huangqi(Astragalus Radix,HQ)-Jinyingzi(Rosae Laevigatae Fructus,JYZ)in the treatment of benign prostatic hyperplasia(BPH)based on network pharmacology a...Objective This study aimed to analyze the mechanism of action of Huangqi(Astragalus Radix,HQ)-Jinyingzi(Rosae Laevigatae Fructus,JYZ)in the treatment of benign prostatic hyperplasia(BPH)based on network pharmacology and to verify the prediction through animal experimentation.Methods Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM)databases,and literature,the active components and related target genes of HQ and JYZ were screened.The BPH target genes were screened based on the DisGeNET and GeneGards databases,and Excel was used to merge and remove duplicates.The Perl language was used to obtain drug-BPH target genes by intersecting shared target genes.A drug-component-target gene network diagram was constructed using Cytoscape software.The drug-BPH intersection target genes were inputted into the STRING database,and the key target genes were selected according to the degree algorithm.The output formed the basis for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses to determine the potential mechanism of HQ and JYZ in BPH treatment.High,medium,and low doses of HQ and JYZ extract were used to intervene in BPH rats,and then the prostate volume,wet weight,and prostate index of the BPH rats were determined.Changes in prostate histopathology and microvessel density(MVD)were evaluated using immunohistochemistry,and the optimal HQ and JYZ extract dose was confirmed.Finally,the optimal dose was used to intervene in a BPH rat model,and AKT1 and VEGF expressions were examined by immunohistochemistry.Results Based on network pharmacology,33 active components and 772 target genes were identified from HQ and JYZ,along with 817 BPH target genes and 112 drug-BPH common target genes.Among them were 10 key target genes,including AKT1,JUN,MAPK1,IL-6,TNF,ESR1,and VEGFA.KEGG enrichment analysis revealed 135 signaling pathways,including PI3K/AKT,I展开更多
Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of H...Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of Huangqi and Danshen ultrafine granule gel on wound vascular healing in rats by network pharmacology and experimental verification.Methods:Using the TCMSP database to screen the active components of two herbs and their potential targets,to search the target related to vascular injury through the Gene Card database,and to select the gene target with correlation coefficient greater than 10%.Then map the disease related target to the potential target of the compound,to obtain the common target,and to import the information to the String online analysis platform and Cytoscape to make the PPI map,and to carry on the topological analysis,KEGG and GO enrichment analysis through Metascape platform.The expression analysis of the main target genes of network pharmacology was verified by pathological section staining and optical density method to determine the content of vascular endothelial growth factor(VEGF)in tissues,the number of tissue new capillaries,and the wound healing rate.Results:A total of 115 active components and 297 target genes were selected from Huangqi and Danshen,and 123 target genes were intersected with vascular injury,among which IL6,AKT1,INS,VEGFA,TNF,EGF,CASP3,MAPK1,TP53 were the active genes with the highest intersection value.The pathways involved are mainly related to AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,Pathways in cancer,human cytomegalovirus infection,Fluid shear stress and atherosclerosis,Endocrine resistance,foxo signaling pathway etc.We experimentally validated VEGFA based on the primary target of vascular regeneration,the experimental results showed that there was different expression of VEGFA proteins in wound vascular healing process,and the quercetin,daidzein,luteolin,apigenin,tanshinone i of 115 active components were selected as the ma展开更多
目的研究黄芪、水蛭及其配方含药血清干预系膜增生性肾小球肾炎的可能作用机制。方法 20只SD大鼠随机分为黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组,每组5只。大鼠给药量5 g/(kg·d)为低浓度,2倍剂量为...目的研究黄芪、水蛭及其配方含药血清干预系膜增生性肾小球肾炎的可能作用机制。方法 20只SD大鼠随机分为黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组,每组5只。大鼠给药量5 g/(kg·d)为低浓度,2倍剂量为高浓度,各组均配成0.5 g/ml混悬液,每天早晚各灌胃1次,连续灌胃5天,末次灌胃1.5 h后制备含药血清。检测各组不同浓度(10%、20%、40%、80%)的含药血清对大鼠系膜细胞增殖的影响,计算细胞存活率,筛选出各组最佳浓度。系膜细胞以1×105个/孔接种于6孔板中,每孔2 ml,分为正常组、模型组、黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组,除正常组外其余各组加入脂多糖诱导大鼠肾小球系膜细胞增殖,再分别加入筛选出的不同浓度的含药血清进行干预,检测各组系膜细胞中核转录因子(NF-кB)、基质金属蛋白酶2(MMP-2)、Fas、Fas L mRNA转录水平。结果黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组含药血清最佳浓度分别为10%、20%、40%、20%。与正常组比较,模型组系膜细胞中NF-кB、MMP-2、Fas、Fas L mRNA表达明显升高(P<0.05),而各含药血清组NF-кB、MMP-2、Fas、Fas L mRNA表达较模型组显著降低(P<0.01)。结论黄芪、水蛭及其配方含药血清可抑制大鼠系膜细胞增殖,其作用机制可能与下调NF-кB、MMP-2、Fas、Fas L mRNA表达水平有关。展开更多
目的:基于网络药理学方法预测补阳还五汤治疗中风的有效成分、作用靶标,探讨多成分-多靶点-多通路的协同作用机制。方法:针对补阳还五汤的方剂组成,采用多元网络药理学方法预测补阳还五汤的体内作用靶标,通过TCMSP等数据库对比补阳还五...目的:基于网络药理学方法预测补阳还五汤治疗中风的有效成分、作用靶标,探讨多成分-多靶点-多通路的协同作用机制。方法:针对补阳还五汤的方剂组成,采用多元网络药理学方法预测补阳还五汤的体内作用靶标,通过TCMSP等数据库对比补阳还五汤治疗中风药物靶标,采用Cytoscape软件构建补阳还五汤-有效成分-疾病靶点调控网络。最后将中药复方—疾病靶标导入Metascape数据库进行基因本体论(gene ontology,GO)富集分析和京都基因与基因组百科全书(kyoto encyclopedia of gene and genome,KEGG)通路富集分析,获得中药-疾病靶标涉及的主要功能和信号通路。结果:通过数据库筛选得到20个补阳还五汤治疗中风的有效成分和136个对应靶点。中药复方-疾病靶标涉及的KEGG通路主要有癌症通路、流体剪切应力与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路、PI3K-Akt信号通路、乙型肝炎病毒、IL-17信号通路、结核病、TNF信号通路、弓形体病、蛋白聚糖在癌症的作用、恰加斯病(美洲锥虫病)、MAPK信号通路、细胞凋亡、非酒精性脂肪肝、甲型流感、细胞因子-受体相互作用、百日咳、HTLV-I感染、HIF-1信号通路及直肠癌等。结论:初步预测了补阳还五汤治疗中风的有效成分与作用机制,为补阳还五汤治疗中风的有效成分与机制研究奠定了基础。展开更多
文摘环黄芪醇作为黄芪中一种极具药用价值的次生代谢产物,对心血管疾病、脑卒中、脂肪肝、腹主动脉瘤等疾病均有保护作用,可激活端粒酶、调节免疫力、促进伤口愈合及毛发生长等。但目前对于环黄芪醇的药理作用研究还存在一些问题:①相关体外实验发现环黄芪醇在一定浓度下具有细胞毒性作用,提示环黄芪醇并非绝对安全,其对细胞的毒性作用尚需深入研究,同时在动物体内的安全性还需进一步验证;②对于环黄芪醇的研究多局限于激活端粒酶这一作用靶点,虽使其在相关保健产品开发中存在优势,但一定程度上限制了对其更深层次和更广范围的研究,目前虽已有环黄芪醇对腺苷酸活化蛋白激酶(AMP activiated protein kinase,AMPK)通路、法尼醇X受体(famesoid X receptor,FXR)转录因子和沉默信息调节蛋白(silent mating type information regulation,SIRT1)蛋白调节作用的探讨,为其药理作用靶点做了一定的补充,但仍缺乏相关的药理活性和作用机制研究。尽管对环黄芪醇的药理作用研究还存在一些问题,但不可否认其药用价值,今后需进一步加强其对心脏纤维化、充血性心力衰竭和心律失常等疾病的保护作用研究,以及对于改善心血管疾病风险指标、调节内皮细胞稳态作用的理论和实验研究,以更好地应用于临床。同时,充分挖掘环黄芪醇在治疗脑缺血和精神类疾病方面的潜力,将其对脑部神经血管疾病的作用作为未来研究的一个新方向。
文摘目的基于网络药理学探讨黄芪治疗类风湿关节炎合并肺间质纤维化的潜在作用机制。方法通过中药系统药理学技术平台(TCMSP)筛选出黄芪中的潜在活性成分,整合TCMSP数据库、靶点预测网站(Swiss Target Prediction)、人类孟德尔遗传数据库(OMIM)、GCBI文献数据库和人类基因数据库(Genecards),预测和筛选其治疗类风湿关节炎合并肺间质纤维化的作用靶点,运用韦恩图(Venny 2.1.0)对药物靶点和疾病靶点进行匹配。通过Systems Dock Web Site对主要活性成分和作用靶点进行分子对接验证。运用String数据库和Cytoscape软件构建绘制蛋白质相互作用网络,并用DisGeNET数据库对作用靶点进行类型归属。采用DAVID数据库对黄芪核心作用靶点进行生物功能和代谢通路分析,借助Cytoscape软件构建"药物-活性成分-核心靶点-通路-疾病"网络。结果筛选得到黄芪25个潜在活性成分,涉及9个作用靶点,与疾病靶点有关的活性成分有3个,主要通过调控TNF、NF-κB、MAPK、Toll样受体、TGF-β信号通路发挥治疗作用。结论黄芪对类风湿关节炎合并肺间质纤维化的治疗作用体现了中药多成分-多靶点-多途径的特点,为阐释其治疗类风湿关节炎合并肺间质纤维化的作用机制提供了科学依据。
基金We thank for the funding support from the Hunan Provincial Science and Technology Department(No.2020JJ4068 and No.2018SK4012).
文摘Objective This study aimed to analyze the mechanism of action of Huangqi(Astragalus Radix,HQ)-Jinyingzi(Rosae Laevigatae Fructus,JYZ)in the treatment of benign prostatic hyperplasia(BPH)based on network pharmacology and to verify the prediction through animal experimentation.Methods Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM)databases,and literature,the active components and related target genes of HQ and JYZ were screened.The BPH target genes were screened based on the DisGeNET and GeneGards databases,and Excel was used to merge and remove duplicates.The Perl language was used to obtain drug-BPH target genes by intersecting shared target genes.A drug-component-target gene network diagram was constructed using Cytoscape software.The drug-BPH intersection target genes were inputted into the STRING database,and the key target genes were selected according to the degree algorithm.The output formed the basis for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses to determine the potential mechanism of HQ and JYZ in BPH treatment.High,medium,and low doses of HQ and JYZ extract were used to intervene in BPH rats,and then the prostate volume,wet weight,and prostate index of the BPH rats were determined.Changes in prostate histopathology and microvessel density(MVD)were evaluated using immunohistochemistry,and the optimal HQ and JYZ extract dose was confirmed.Finally,the optimal dose was used to intervene in a BPH rat model,and AKT1 and VEGF expressions were examined by immunohistochemistry.Results Based on network pharmacology,33 active components and 772 target genes were identified from HQ and JYZ,along with 817 BPH target genes and 112 drug-BPH common target genes.Among them were 10 key target genes,including AKT1,JUN,MAPK1,IL-6,TNF,ESR1,and VEGFA.KEGG enrichment analysis revealed 135 signaling pathways,including PI3K/AKT,I
文摘Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of Huangqi and Danshen ultrafine granule gel on wound vascular healing in rats by network pharmacology and experimental verification.Methods:Using the TCMSP database to screen the active components of two herbs and their potential targets,to search the target related to vascular injury through the Gene Card database,and to select the gene target with correlation coefficient greater than 10%.Then map the disease related target to the potential target of the compound,to obtain the common target,and to import the information to the String online analysis platform and Cytoscape to make the PPI map,and to carry on the topological analysis,KEGG and GO enrichment analysis through Metascape platform.The expression analysis of the main target genes of network pharmacology was verified by pathological section staining and optical density method to determine the content of vascular endothelial growth factor(VEGF)in tissues,the number of tissue new capillaries,and the wound healing rate.Results:A total of 115 active components and 297 target genes were selected from Huangqi and Danshen,and 123 target genes were intersected with vascular injury,among which IL6,AKT1,INS,VEGFA,TNF,EGF,CASP3,MAPK1,TP53 were the active genes with the highest intersection value.The pathways involved are mainly related to AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,Pathways in cancer,human cytomegalovirus infection,Fluid shear stress and atherosclerosis,Endocrine resistance,foxo signaling pathway etc.We experimentally validated VEGFA based on the primary target of vascular regeneration,the experimental results showed that there was different expression of VEGFA proteins in wound vascular healing process,and the quercetin,daidzein,luteolin,apigenin,tanshinone i of 115 active components were selected as the ma
文摘目的研究黄芪、水蛭及其配方含药血清干预系膜增生性肾小球肾炎的可能作用机制。方法 20只SD大鼠随机分为黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组,每组5只。大鼠给药量5 g/(kg·d)为低浓度,2倍剂量为高浓度,各组均配成0.5 g/ml混悬液,每天早晚各灌胃1次,连续灌胃5天,末次灌胃1.5 h后制备含药血清。检测各组不同浓度(10%、20%、40%、80%)的含药血清对大鼠系膜细胞增殖的影响,计算细胞存活率,筛选出各组最佳浓度。系膜细胞以1×105个/孔接种于6孔板中,每孔2 ml,分为正常组、模型组、黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组,除正常组外其余各组加入脂多糖诱导大鼠肾小球系膜细胞增殖,再分别加入筛选出的不同浓度的含药血清进行干预,检测各组系膜细胞中核转录因子(NF-кB)、基质金属蛋白酶2(MMP-2)、Fas、Fas L mRNA转录水平。结果黄芪高血清组、水蛭高血清组、黄芪高水蛭高血清组、黄芪低水蛭高血清组含药血清最佳浓度分别为10%、20%、40%、20%。与正常组比较,模型组系膜细胞中NF-кB、MMP-2、Fas、Fas L mRNA表达明显升高(P<0.05),而各含药血清组NF-кB、MMP-2、Fas、Fas L mRNA表达较模型组显著降低(P<0.01)。结论黄芪、水蛭及其配方含药血清可抑制大鼠系膜细胞增殖,其作用机制可能与下调NF-кB、MMP-2、Fas、Fas L mRNA表达水平有关。
文摘目的:基于网络药理学方法预测补阳还五汤治疗中风的有效成分、作用靶标,探讨多成分-多靶点-多通路的协同作用机制。方法:针对补阳还五汤的方剂组成,采用多元网络药理学方法预测补阳还五汤的体内作用靶标,通过TCMSP等数据库对比补阳还五汤治疗中风药物靶标,采用Cytoscape软件构建补阳还五汤-有效成分-疾病靶点调控网络。最后将中药复方—疾病靶标导入Metascape数据库进行基因本体论(gene ontology,GO)富集分析和京都基因与基因组百科全书(kyoto encyclopedia of gene and genome,KEGG)通路富集分析,获得中药-疾病靶标涉及的主要功能和信号通路。结果:通过数据库筛选得到20个补阳还五汤治疗中风的有效成分和136个对应靶点。中药复方-疾病靶标涉及的KEGG通路主要有癌症通路、流体剪切应力与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路、PI3K-Akt信号通路、乙型肝炎病毒、IL-17信号通路、结核病、TNF信号通路、弓形体病、蛋白聚糖在癌症的作用、恰加斯病(美洲锥虫病)、MAPK信号通路、细胞凋亡、非酒精性脂肪肝、甲型流感、细胞因子-受体相互作用、百日咳、HTLV-I感染、HIF-1信号通路及直肠癌等。结论:初步预测了补阳还五汤治疗中风的有效成分与作用机制,为补阳还五汤治疗中风的有效成分与机制研究奠定了基础。
