AIM TO uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism.METHODS Endogenous zinc finger E-box binding homeobox-1 （ZEB1） was silenced...AIM TO uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism.METHODS Endogenous zinc finger E-box binding homeobox-1 （ZEB1） was silenced using a and the impact of ZEB1 and lentivirus-mediated method, methyI-CpG binding domain protein 1 （MBD1） on aerobic glycolysis was measured using seahorse cellular flux analyzers, reactive oxygen species quantification, and mitochondrial membrane potential measurement. The interaction between ZEB1 and MBD1 was assessed by co-immunoprecipitation and immunofluorescence assays. The impact of ZEB1 and MBD1 interaction on sirtuin 3 （SIRT3） expression was confirmed by quantitative polymerase chain reaction, western blotting, and dual-luciferase and chromatinimmunoprecipitation assays.RESULTS ZEB1 was a positive regulator of aerobic glycolysis in pancreatic cancer. ZEB1 transcriptionally silenced expression of SIRT3, a mitochondrial-localized tumor suppressor, through interaction with MBD1.CONCLUSION ZEB1 silenced SIRT3 expression via interaction with MBD1 to promote aerobic glycolysis in pancreatic cancer.展开更多
Objective: To study the change of homeobox protein 7 (HOXB7) gene expression in colon cancer lesions and its correlation with the pathological characteristics of tumor. Methods: The patients with colon cancer who unde...Objective: To study the change of homeobox protein 7 (HOXB7) gene expression in colon cancer lesions and its correlation with the pathological characteristics of tumor. Methods: The patients with colon cancer who underwent radical resection in Tangshan Hospital of Traditional Chinese Medicine between March 2015 and February 2018 were selected as the research subjects, and right amount of colon cancer lesions and lesions adjacent to carcinoma were collected to measure the expression of HOXB7 gene, cell proliferation-related genes Notch1, Hes1, P53, ASPP2 and WTX as well as invasion-related genes CAT-D, TMEM16a, CBX7, ZEB1, E-cadherin and N-cadherin. Results: HOXB7, Notch1, Hes1, CAT-D, TMEM16a, CBX7, ZEB1 and N-cadherin mRNA expression in colon cancer lesions were higher than those in adjacent lesions whereas P53, ASPP2, WTX and E-cadherin mRNA expression were lower than those in adjacent lesions;the mRNA of HOXB7 gene in colon cancer was positively correlated with the mRNA expression of Notch1, Hes1, CAT-D, TMEM16a, CBX7, ZEB1 and N-cadherin, and negatively correlated with the mRNA expression of P53, ASPP2, WTX and E-cadherin. Conclusion: The high expression of HOXB7 gene in the colon cancer lesions can enhance the pathological characteristics of tumor proliferation and invasion.展开更多
BACKGROUND Cholangiocarcinoma(CCA)represents a rare but highly aggressive malignancy that is often challenging to diagnose,especially in early stages.The role of existing tumor biomarkers for CCA diagnosis,remains con...BACKGROUND Cholangiocarcinoma(CCA)represents a rare but highly aggressive malignancy that is often challenging to diagnose,especially in early stages.The role of existing tumor biomarkers for CCA diagnosis,remains controversial due to their low sensitivity and specificity.Increasing evidence has implicated long non-coding ribonucleic acid polymorphisms with cancer susceptibility in a variety of tumor types.The association between long non-coding ribonucleic acid homeobox protein transcript antisense intergenic ribonucleic acid(HOTAIR)polymorphisms and CCA risk has not been reported yet.AIM To investigate the influence of HOTAIR variants on the risk of CCA development.METHODS We conducted a case-control study in which three HOTAIR single nucleotide polymorphisms(rs920778,rs4759314 and rs7958904)were genotyped in a Greek cohort.Our study population included 122 CCA patients(80 males and 42 females)and 165 healthy controls.The polymorphisms under investigation were examined in peripheral blood samples.RESULTS HOTAIR rs4759314 AG and GG genotypes were associated with a significantly increased CCA risk[P=0.004,odds ratio:3.13;95%confidence interval:1.65-5.91 and P=0.005,odds ratio:12.31;95%confidence interval:1.48-101.87,respectively].However,no significant associations of HOTAIR rs920778,and rs7958904 were detected.Similarly,we found no significant associations between rs4759314 AA genotype and CCA susceptibility.CONCLUSION HOTAIR rs4759314 AG and GG genotypes may be implicated with CCA development and may serve as a potential diagnostic biomarker.展开更多
BACKGROUND Neuroblastoma(NB)is one of the most common malignancies in children.Metastasis in NB is not uncommon.However,nasal metastases are rare.Here,we reported two pediatric cases of nasal metastases.CASE SUMMARY C...BACKGROUND Neuroblastoma(NB)is one of the most common malignancies in children.Metastasis in NB is not uncommon.However,nasal metastases are rare.Here,we reported two pediatric cases of nasal metastases.CASE SUMMARY Case 1 was a 3-year-old boy without a history of NB.Case 2 was a 10-year-old girl who had a history of NB for 6 years.Both of them presented with symptoms of nasal and sinus masses such as epistaxis or discharge from the nose.The radiologic imaging results revealed masses in the nasal cavity or nasopharynx in both cases and a mass in the right adrenal gland of case 1.The pathologic examination of biopsy samples of their nasal masses revealed“small round bluecell tumor”along with abundant vascular fibrous septa.The tumor cells expressed synaptophysin,cluster of differentiation 56,chromogranin A,paired like homeobox protein 2B and a very high Ki67 index in both case but were negative for vimentin,desmin,leucocyte common antigen and cytokeratin.Myelocytomatosis viral related oncogene,neuroblastoma derived(MYCN)amplification was detected in both cases.Finally,the two cases were diagnosed as nasal metastases from NB based on the clinical and pathologic findings.The two patients affected by NB were>18 mo old,the primary tumor location was adrenal gland,and they presented with multiple metastases.CONCLUSION It is difficult to differentiate between metastatic NB in the nose and olfactory neuroblastoma in the absence of a history of NB.Paired like homeobox protein 2B can play an important role in the diagnosis and differential diagnosis of this disease.展开更多
目的探讨组织尾侧型同源转录因子2(Caudal type homeobox transcription factor 2,CDX2)、肝细胞核因子4α(Hepatocyte nuclear factor 4 alpha,HNF4α)、腺苷酸环化酶相关蛋白2(Adenylate cyclase related protein 2,CAP2)联合检测对...目的探讨组织尾侧型同源转录因子2(Caudal type homeobox transcription factor 2,CDX2)、肝细胞核因子4α(Hepatocyte nuclear factor 4 alpha,HNF4α)、腺苷酸环化酶相关蛋白2(Adenylate cyclase related protein 2,CAP2)联合检测对幽门螺杆菌(Hp)感染相关高危胃癌患者的诊断价值。方法收集2017年2月-2019年1月胜利油田中心医院收治的47例萎缩性胃炎及59例胃癌患者胃黏膜组织标本;免疫组化染色测定组织CDX2、HNF4α、CAP2蛋白表达情况,快速银染法检测Hp感染情况,分析三者在Hp感染相关胃部炎症恶性转化过程中的作用,受试者工作曲线(Receiver operator characteristic curve,ROC)分析三者联合检测对Hp感染相关胃癌的诊断价值。结果胃癌患者组织标本CDX2阳性率低于萎缩性胃癌患者(P<0.05),HNF4α、CAP2阳性率高于萎缩性胃炎患者(P<0.05);胃癌患者Hp感染率为79.66%,高于萎缩性胃炎Hp感染率42.55%(P<0.05),Hp阳性胃癌、萎缩性胃炎组织标本CDX2、HNF4α、CAP2阳性率皆高于Hp阴性病例组织标本(P<0.05);胃癌患者组织CDX2、HNF4α、CAP2表达与Hp感染率呈正相关(P<0.05);HNF4α阳性染色评分>3分预测Hp相关高危胃癌价值最高,曲线下面积(Area under the curve,AUC)为0.870,敏感度、特异性分别为91.49%、75.00%;其次为CAP2,AUC为0.860,敏感度、特异性为89.36%、75.00%;三者联合预测Hp相关高危胃癌综合效能最高,AUC为0.891,敏感度、特异性分别为89.36%、91.67%。结论组织CDX2、HNF4α、CAP2异常表达与Hp感染有关,参与胃炎向胃癌恶性转化过程;联合检测组织CDX2、HNF4α、CAP2对Hp感染相关高危胃癌有较高的预测价值。展开更多
基金the National Science Fund for Distinguished Young Scholars of China,No.81625016the National Science Foundation of China,No.81502031 and No.81772555+1 种基金Shanghai Municipal Commission of Health and Family Planning Grant,No.20154Y0090Youth Research Foundation of Shanghai Municipal Commission of Health and Family Planning,No.Z0124Y074
文摘AIM TO uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism.METHODS Endogenous zinc finger E-box binding homeobox-1 （ZEB1） was silenced using a and the impact of ZEB1 and lentivirus-mediated method, methyI-CpG binding domain protein 1 （MBD1） on aerobic glycolysis was measured using seahorse cellular flux analyzers, reactive oxygen species quantification, and mitochondrial membrane potential measurement. The interaction between ZEB1 and MBD1 was assessed by co-immunoprecipitation and immunofluorescence assays. The impact of ZEB1 and MBD1 interaction on sirtuin 3 （SIRT3） expression was confirmed by quantitative polymerase chain reaction, western blotting, and dual-luciferase and chromatinimmunoprecipitation assays.RESULTS ZEB1 was a positive regulator of aerobic glycolysis in pancreatic cancer. ZEB1 transcriptionally silenced expression of SIRT3, a mitochondrial-localized tumor suppressor, through interaction with MBD1.CONCLUSION ZEB1 silenced SIRT3 expression via interaction with MBD1 to promote aerobic glycolysis in pancreatic cancer.
文摘Objective: To study the change of homeobox protein 7 (HOXB7) gene expression in colon cancer lesions and its correlation with the pathological characteristics of tumor. Methods: The patients with colon cancer who underwent radical resection in Tangshan Hospital of Traditional Chinese Medicine between March 2015 and February 2018 were selected as the research subjects, and right amount of colon cancer lesions and lesions adjacent to carcinoma were collected to measure the expression of HOXB7 gene, cell proliferation-related genes Notch1, Hes1, P53, ASPP2 and WTX as well as invasion-related genes CAT-D, TMEM16a, CBX7, ZEB1, E-cadherin and N-cadherin. Results: HOXB7, Notch1, Hes1, CAT-D, TMEM16a, CBX7, ZEB1 and N-cadherin mRNA expression in colon cancer lesions were higher than those in adjacent lesions whereas P53, ASPP2, WTX and E-cadherin mRNA expression were lower than those in adjacent lesions;the mRNA of HOXB7 gene in colon cancer was positively correlated with the mRNA expression of Notch1, Hes1, CAT-D, TMEM16a, CBX7, ZEB1 and N-cadherin, and negatively correlated with the mRNA expression of P53, ASPP2, WTX and E-cadherin. Conclusion: The high expression of HOXB7 gene in the colon cancer lesions can enhance the pathological characteristics of tumor proliferation and invasion.
基金The Hellenic Society of Medical Oncology,No.8021/25.09.2020.
文摘BACKGROUND Cholangiocarcinoma(CCA)represents a rare but highly aggressive malignancy that is often challenging to diagnose,especially in early stages.The role of existing tumor biomarkers for CCA diagnosis,remains controversial due to their low sensitivity and specificity.Increasing evidence has implicated long non-coding ribonucleic acid polymorphisms with cancer susceptibility in a variety of tumor types.The association between long non-coding ribonucleic acid homeobox protein transcript antisense intergenic ribonucleic acid(HOTAIR)polymorphisms and CCA risk has not been reported yet.AIM To investigate the influence of HOTAIR variants on the risk of CCA development.METHODS We conducted a case-control study in which three HOTAIR single nucleotide polymorphisms(rs920778,rs4759314 and rs7958904)were genotyped in a Greek cohort.Our study population included 122 CCA patients(80 males and 42 females)and 165 healthy controls.The polymorphisms under investigation were examined in peripheral blood samples.RESULTS HOTAIR rs4759314 AG and GG genotypes were associated with a significantly increased CCA risk[P=0.004,odds ratio:3.13;95%confidence interval:1.65-5.91 and P=0.005,odds ratio:12.31;95%confidence interval:1.48-101.87,respectively].However,no significant associations of HOTAIR rs920778,and rs7958904 were detected.Similarly,we found no significant associations between rs4759314 AA genotype and CCA susceptibility.CONCLUSION HOTAIR rs4759314 AG and GG genotypes may be implicated with CCA development and may serve as a potential diagnostic biomarker.
文摘BACKGROUND Neuroblastoma(NB)is one of the most common malignancies in children.Metastasis in NB is not uncommon.However,nasal metastases are rare.Here,we reported two pediatric cases of nasal metastases.CASE SUMMARY Case 1 was a 3-year-old boy without a history of NB.Case 2 was a 10-year-old girl who had a history of NB for 6 years.Both of them presented with symptoms of nasal and sinus masses such as epistaxis or discharge from the nose.The radiologic imaging results revealed masses in the nasal cavity or nasopharynx in both cases and a mass in the right adrenal gland of case 1.The pathologic examination of biopsy samples of their nasal masses revealed“small round bluecell tumor”along with abundant vascular fibrous septa.The tumor cells expressed synaptophysin,cluster of differentiation 56,chromogranin A,paired like homeobox protein 2B and a very high Ki67 index in both case but were negative for vimentin,desmin,leucocyte common antigen and cytokeratin.Myelocytomatosis viral related oncogene,neuroblastoma derived(MYCN)amplification was detected in both cases.Finally,the two cases were diagnosed as nasal metastases from NB based on the clinical and pathologic findings.The two patients affected by NB were>18 mo old,the primary tumor location was adrenal gland,and they presented with multiple metastases.CONCLUSION It is difficult to differentiate between metastatic NB in the nose and olfactory neuroblastoma in the absence of a history of NB.Paired like homeobox protein 2B can play an important role in the diagnosis and differential diagnosis of this disease.
文摘目的探讨组织尾侧型同源转录因子2(Caudal type homeobox transcription factor 2,CDX2)、肝细胞核因子4α(Hepatocyte nuclear factor 4 alpha,HNF4α)、腺苷酸环化酶相关蛋白2(Adenylate cyclase related protein 2,CAP2)联合检测对幽门螺杆菌(Hp)感染相关高危胃癌患者的诊断价值。方法收集2017年2月-2019年1月胜利油田中心医院收治的47例萎缩性胃炎及59例胃癌患者胃黏膜组织标本;免疫组化染色测定组织CDX2、HNF4α、CAP2蛋白表达情况,快速银染法检测Hp感染情况,分析三者在Hp感染相关胃部炎症恶性转化过程中的作用,受试者工作曲线(Receiver operator characteristic curve,ROC)分析三者联合检测对Hp感染相关胃癌的诊断价值。结果胃癌患者组织标本CDX2阳性率低于萎缩性胃癌患者(P<0.05),HNF4α、CAP2阳性率高于萎缩性胃炎患者(P<0.05);胃癌患者Hp感染率为79.66%,高于萎缩性胃炎Hp感染率42.55%(P<0.05),Hp阳性胃癌、萎缩性胃炎组织标本CDX2、HNF4α、CAP2阳性率皆高于Hp阴性病例组织标本(P<0.05);胃癌患者组织CDX2、HNF4α、CAP2表达与Hp感染率呈正相关(P<0.05);HNF4α阳性染色评分>3分预测Hp相关高危胃癌价值最高,曲线下面积(Area under the curve,AUC)为0.870,敏感度、特异性分别为91.49%、75.00%;其次为CAP2,AUC为0.860,敏感度、特异性为89.36%、75.00%;三者联合预测Hp相关高危胃癌综合效能最高,AUC为0.891,敏感度、特异性分别为89.36%、91.67%。结论组织CDX2、HNF4α、CAP2异常表达与Hp感染有关,参与胃炎向胃癌恶性转化过程;联合检测组织CDX2、HNF4α、CAP2对Hp感染相关高危胃癌有较高的预测价值。
