Background:Hodgkin lymphoma refers to a malignancy of the lymphatic tissue.Extra-nodal Hodgkin lymphoma(ENHL)is a rare variant consisting of Hodgkin lymphoma occurring outside the lymphatic system.Studies investigatin...Background:Hodgkin lymphoma refers to a malignancy of the lymphatic tissue.Extra-nodal Hodgkin lymphoma(ENHL)is a rare variant consisting of Hodgkin lymphoma occurring outside the lymphatic system.Studies investigating the epidemiology associated with ENHL are rare.Methods:618 ENHL cases were analyzed using the National Cancer Institute’s Surveillance,Epidemiology,and End Results(SEER)database(2000–2020).Demographics including sex,race/ethnicity,rural-urban continuum,age group(categorized as adolescent and young adult(AYA)if between years of 15 and 39,and adult if over the age of 40),and living status(alive/deceased)were examined,with survival status as the main outcome.Results:This study included 335 males(54.2%)and 283 females(45.8%).Of this total,47.9%were in the AYA group.In terms of ethnicity the distribution was 12.5%non-Hispanic Black,67.5%non-Hispanic White,5.2%non-Hispanic Asian/Pacific Islander,and 14.9%Hispanic.Bivariate analyses evidenced significant differences in survival by age group with 91.6%in AYA vs.72.4%in adults(P<0.001)alive by the end of the study period.Multivariable analyses identified age as a key predictor of survival,as the AYA patients had a lower odds ratio for death(odds ratio=0.25,P<0.001).In addition,survival outcomes were also impacted by race,with non-Hispanic Blacks showing higher survival probabilities.Regarding treatment,27.0%of patients underwent surgery,with 10.2%receiving post-surgery radiation,reducing odds of mortality(odds ratio=0.32,P=0.046).Conclusion:The background research as such,tends to affirm that these two factors–age and race are quite crucial in the prognosis as well as management of ENHL.Compared to adults,AYA patients had significantly lower odds of death,while non-Hispanic Black individuals exhibited reduced survival probabilities.It should be noted that 27.0%of patients underwent surgery with 10.2%receiving post-operative radiation which led to decrease in mortality rates.Thus,these results reiterate the necessity for tailor-made treatment methods a展开更多
目的 探讨结核抗原Ag85作用于巨噬细胞后对霍奇金淋巴瘤细胞增殖、凋亡的影响,以及结核感染在霍奇金淋巴瘤进展中的可能作用。 方法 采用Transwell嵌套法建立霍奇金淋巴瘤细胞株KM-H2与人单核细胞白血病细胞株THP-1(模拟巨噬细胞)非直...目的 探讨结核抗原Ag85作用于巨噬细胞后对霍奇金淋巴瘤细胞增殖、凋亡的影响,以及结核感染在霍奇金淋巴瘤进展中的可能作用。 方法 采用Transwell嵌套法建立霍奇金淋巴瘤细胞株KM-H2与人单核细胞白血病细胞株THP-1(模拟巨噬细胞)非直接接触共培养体系。KM-H2细胞单独培养为KM-H2组,Ag85干预的KM-H2细胞为KM-H2+Ag85组,KM-H2细胞与THP-1细胞共培养为KM-H2+THP-1组,Ag85干预的KM-H2细胞与THP-1细胞共培养为KM-H2+THP-1+Ag85组。采用CCK-8法检测各组KM-H2细胞的增殖情况,绘制生长曲线;采用流式细胞术检测各组细胞的凋亡情况;采用实时荧光定量聚合酶链反应(qRT-PCR)检测各组细胞p53、c-myc、bcl-2、血管内皮生长因子受体3(VEGFR3)mRNA表达水平;采用蛋白质印迹法检测各组细胞bax、bcl-2蛋白的表达。 结果 培养24、48 h后KM-H2+Ag85组细胞增殖能力均高于KM-H2组(均 P =0.001),而培养24、48、72 h后KM-H2+THP-1组细胞增殖能力均低于KM-H2组(均 P <0.05);培养48、72 h后KM-H2+THP-1+Ag85组细胞增殖能力均低于KM-H2组(均 P <0.05),但与KM-H2+THP-1组相比,培养24、48 h后KM-H2+THP-1+Ag85组细胞增殖能力均增强,培养72h后两组间差异无统计学意义( P >0.05)。KM-H2+Ag85组细胞凋亡率[(0.92±0.80)%]低于KM-H2组[(6.02±1.63)%]( P <0.001),KM-H2+THP-1组细胞凋亡率[(8.57±0.57)%]高于KM-H2组( P <0.05),而KM-H2+THP-1+Ag85组细胞凋亡率[(0.60±0.13)%]较KM-H2+THP-1组降低( P <0.001)。KM-H2+Ag85组细胞bcl-2、VEGFR3 mRNA相对表达量均高于KM-H2组( P 值分别为0.018、0.017),c-myc mRNA相对表达量低于KM-H2组( P =0.016),两组间p53 mRNA相对表达量差异无统计学意义( P >0.05);KM-H2+THP-1+Ag85组细胞p53 mRNA相对表达量低于KM-H2+THP-1组( P =0.048),bcl-2、VEGFR3 mRNA相对表达量均高于KM-H2+THP-1组( P 值分别为0.016、0.021)。KM-H2+Ag85组细胞bax蛋白表达较KM-H2组降低( P =0.019),bcl-2蛋白表达较KM-H2组增加( P =0.001);KM-H2+THP-1+Ag展开更多
文摘Background:Hodgkin lymphoma refers to a malignancy of the lymphatic tissue.Extra-nodal Hodgkin lymphoma(ENHL)is a rare variant consisting of Hodgkin lymphoma occurring outside the lymphatic system.Studies investigating the epidemiology associated with ENHL are rare.Methods:618 ENHL cases were analyzed using the National Cancer Institute’s Surveillance,Epidemiology,and End Results(SEER)database(2000–2020).Demographics including sex,race/ethnicity,rural-urban continuum,age group(categorized as adolescent and young adult(AYA)if between years of 15 and 39,and adult if over the age of 40),and living status(alive/deceased)were examined,with survival status as the main outcome.Results:This study included 335 males(54.2%)and 283 females(45.8%).Of this total,47.9%were in the AYA group.In terms of ethnicity the distribution was 12.5%non-Hispanic Black,67.5%non-Hispanic White,5.2%non-Hispanic Asian/Pacific Islander,and 14.9%Hispanic.Bivariate analyses evidenced significant differences in survival by age group with 91.6%in AYA vs.72.4%in adults(P<0.001)alive by the end of the study period.Multivariable analyses identified age as a key predictor of survival,as the AYA patients had a lower odds ratio for death(odds ratio=0.25,P<0.001).In addition,survival outcomes were also impacted by race,with non-Hispanic Blacks showing higher survival probabilities.Regarding treatment,27.0%of patients underwent surgery,with 10.2%receiving post-surgery radiation,reducing odds of mortality(odds ratio=0.32,P=0.046).Conclusion:The background research as such,tends to affirm that these two factors–age and race are quite crucial in the prognosis as well as management of ENHL.Compared to adults,AYA patients had significantly lower odds of death,while non-Hispanic Black individuals exhibited reduced survival probabilities.It should be noted that 27.0%of patients underwent surgery with 10.2%receiving post-operative radiation which led to decrease in mortality rates.Thus,these results reiterate the necessity for tailor-made treatment methods a
文摘目的 探讨结核抗原Ag85作用于巨噬细胞后对霍奇金淋巴瘤细胞增殖、凋亡的影响,以及结核感染在霍奇金淋巴瘤进展中的可能作用。 方法 采用Transwell嵌套法建立霍奇金淋巴瘤细胞株KM-H2与人单核细胞白血病细胞株THP-1(模拟巨噬细胞)非直接接触共培养体系。KM-H2细胞单独培养为KM-H2组,Ag85干预的KM-H2细胞为KM-H2+Ag85组,KM-H2细胞与THP-1细胞共培养为KM-H2+THP-1组,Ag85干预的KM-H2细胞与THP-1细胞共培养为KM-H2+THP-1+Ag85组。采用CCK-8法检测各组KM-H2细胞的增殖情况,绘制生长曲线;采用流式细胞术检测各组细胞的凋亡情况;采用实时荧光定量聚合酶链反应(qRT-PCR)检测各组细胞p53、c-myc、bcl-2、血管内皮生长因子受体3(VEGFR3)mRNA表达水平;采用蛋白质印迹法检测各组细胞bax、bcl-2蛋白的表达。 结果 培养24、48 h后KM-H2+Ag85组细胞增殖能力均高于KM-H2组(均 P =0.001),而培养24、48、72 h后KM-H2+THP-1组细胞增殖能力均低于KM-H2组(均 P <0.05);培养48、72 h后KM-H2+THP-1+Ag85组细胞增殖能力均低于KM-H2组(均 P <0.05),但与KM-H2+THP-1组相比,培养24、48 h后KM-H2+THP-1+Ag85组细胞增殖能力均增强,培养72h后两组间差异无统计学意义( P >0.05)。KM-H2+Ag85组细胞凋亡率[(0.92±0.80)%]低于KM-H2组[(6.02±1.63)%]( P <0.001),KM-H2+THP-1组细胞凋亡率[(8.57±0.57)%]高于KM-H2组( P <0.05),而KM-H2+THP-1+Ag85组细胞凋亡率[(0.60±0.13)%]较KM-H2+THP-1组降低( P <0.001)。KM-H2+Ag85组细胞bcl-2、VEGFR3 mRNA相对表达量均高于KM-H2组( P 值分别为0.018、0.017),c-myc mRNA相对表达量低于KM-H2组( P =0.016),两组间p53 mRNA相对表达量差异无统计学意义( P >0.05);KM-H2+THP-1+Ag85组细胞p53 mRNA相对表达量低于KM-H2+THP-1组( P =0.048),bcl-2、VEGFR3 mRNA相对表达量均高于KM-H2+THP-1组( P 值分别为0.016、0.021)。KM-H2+Ag85组细胞bax蛋白表达较KM-H2组降低( P =0.019),bcl-2蛋白表达较KM-H2组增加( P =0.001);KM-H2+THP-1+Ag
