【目的】解析高产广适小麦新品种淮麦33的遗传构成,探讨双亲烟农19和郑麦991对其产量相关性状的遗传贡献,为小麦品种改良及亲本选配提供依据。【方法】利用部分农艺及品质性状、高分子量谷蛋白亚基组成及覆盖小麦21条染色体的625个SSR...【目的】解析高产广适小麦新品种淮麦33的遗传构成,探讨双亲烟农19和郑麦991对其产量相关性状的遗传贡献,为小麦品种改良及亲本选配提供依据。【方法】利用部分农艺及品质性状、高分子量谷蛋白亚基组成及覆盖小麦21条染色体的625个SSR分子标记分析淮麦33及其双亲的遗传构成;比对已知的产量性状相关QTL,分析双亲的产量相关区段对淮麦33的遗传贡献。【结果】淮麦33的每平方米穗数和千粒重均介于两亲本之间,穗粒数和小区产量均显著高于两亲本;与烟农19相比,其株高显著降低。淮麦33的高分子量谷蛋白亚基组成为(1、17+18和2+12),其中1和17+18亚基均来自于母本烟农19,2+12亚基来自于父本郑麦991。SSR分子标记分析表明,双亲对淮麦33的遗传贡献和理论值相比出现了较大偏离,淮麦33分别继承了烟农19和郑麦991两亲本73.9%和26.1%的遗传物质。淮麦33与烟农19具有较大的遗传相似度,遗传相似系数为0.78。在不同基因组和染色体水平上,双亲对淮麦33的遗传贡献率差异较大,其中,烟农19在A、B和D基因组水平的遗传贡献率均较高,分别为75.1%、69.4%和68.7%;除6A染色体外,烟农19在其他20条染色体上的遗传贡献率均高于郑麦991,其中在2A染色体上达到100%,在1A、3A、2B、3B和4B等5条染色体上均超过90%。在遗传距离大于5 c M的染色体区段中,淮麦33来源于烟农19和郑麦991的染色体区段分别有34个和7个,其中在2D染色体上来源于烟农19的染色体区段最多,在5A染色体上来源于郑麦991的区段最多。淮麦33有38个不同于双亲的特异位点,主要分布在1B、1D、2A、2B、2D、3A、3B、3D、4A、4B、5A、5B、6B、6D和7A等15条染色体上。比对已知产量性状相关QTL,共发现10个产量相关区段,有6个来源于烟农19,分别位于1A、2D、3B、4B、4D和7A染色体上;3个来源于郑麦991,分别位于4A和5A染色体上;1个为淮麦33特异区段,�展开更多
Sedimentation values, flour glutenin macropolymer (GMP) contents, composition and contents of high-molecular-weight (HMW) glutenin subunits (GS) of 233 flour samples were determined. Our data indicated that subunit 1 ...Sedimentation values, flour glutenin macropolymer (GMP) contents, composition and contents of high-molecular-weight (HMW) glutenin subunits (GS) of 233 flour samples were determined. Our data indicated that subunit 1 occurred more frequently at Glu-A1 , subunit pair 7 + 8 at Glu-B1 and 2 + 12 at Glu-D1. The significant relationships between Glu-1 quality score and total HMW glutenin content, sedimentation value and GMP content suggested that the composition of HMW-GS affects wheat quality strongly. Moreover, the total content of HMW-GS was correlated with certain quality parameters more significantly. Relationship between subunit 5 + 10 content and breadmaking quality was better than others, but 2 + 12, 7 + 8, 7 + 9 and 4 + 12 also correlated with certain quality parameters significantly. The contents of total HMW-glutenin, x-type subunits and y-type subunits related with sedimentation value, flour GMP content, and Glu-1 quality score more strongly than that of individual subunit or subunit pair. The flour GMP content, with excellent correlation to sedimentation value, total contents of HMW glutenin, x- and y-type subunits and many other quality parameters, could be an ideal indicator of breadmaking quality at earlier generations for breeding purpose for its simple procedure and small scale.展开更多
文摘【目的】解析高产广适小麦新品种淮麦33的遗传构成,探讨双亲烟农19和郑麦991对其产量相关性状的遗传贡献,为小麦品种改良及亲本选配提供依据。【方法】利用部分农艺及品质性状、高分子量谷蛋白亚基组成及覆盖小麦21条染色体的625个SSR分子标记分析淮麦33及其双亲的遗传构成;比对已知的产量性状相关QTL,分析双亲的产量相关区段对淮麦33的遗传贡献。【结果】淮麦33的每平方米穗数和千粒重均介于两亲本之间,穗粒数和小区产量均显著高于两亲本;与烟农19相比,其株高显著降低。淮麦33的高分子量谷蛋白亚基组成为(1、17+18和2+12),其中1和17+18亚基均来自于母本烟农19,2+12亚基来自于父本郑麦991。SSR分子标记分析表明,双亲对淮麦33的遗传贡献和理论值相比出现了较大偏离,淮麦33分别继承了烟农19和郑麦991两亲本73.9%和26.1%的遗传物质。淮麦33与烟农19具有较大的遗传相似度,遗传相似系数为0.78。在不同基因组和染色体水平上,双亲对淮麦33的遗传贡献率差异较大,其中,烟农19在A、B和D基因组水平的遗传贡献率均较高,分别为75.1%、69.4%和68.7%;除6A染色体外,烟农19在其他20条染色体上的遗传贡献率均高于郑麦991,其中在2A染色体上达到100%,在1A、3A、2B、3B和4B等5条染色体上均超过90%。在遗传距离大于5 c M的染色体区段中,淮麦33来源于烟农19和郑麦991的染色体区段分别有34个和7个,其中在2D染色体上来源于烟农19的染色体区段最多,在5A染色体上来源于郑麦991的区段最多。淮麦33有38个不同于双亲的特异位点,主要分布在1B、1D、2A、2B、2D、3A、3B、3D、4A、4B、5A、5B、6B、6D和7A等15条染色体上。比对已知产量性状相关QTL,共发现10个产量相关区段,有6个来源于烟农19,分别位于1A、2D、3B、4B、4D和7A染色体上;3个来源于郑麦991,分别位于4A和5A染色体上;1个为淮麦33特异区段,�
基金the National Natural Science Foundation of China(No.39970456 ,39930110).
文摘Sedimentation values, flour glutenin macropolymer (GMP) contents, composition and contents of high-molecular-weight (HMW) glutenin subunits (GS) of 233 flour samples were determined. Our data indicated that subunit 1 occurred more frequently at Glu-A1 , subunit pair 7 + 8 at Glu-B1 and 2 + 12 at Glu-D1. The significant relationships between Glu-1 quality score and total HMW glutenin content, sedimentation value and GMP content suggested that the composition of HMW-GS affects wheat quality strongly. Moreover, the total content of HMW-GS was correlated with certain quality parameters more significantly. Relationship between subunit 5 + 10 content and breadmaking quality was better than others, but 2 + 12, 7 + 8, 7 + 9 and 4 + 12 also correlated with certain quality parameters significantly. The contents of total HMW-glutenin, x-type subunits and y-type subunits related with sedimentation value, flour GMP content, and Glu-1 quality score more strongly than that of individual subunit or subunit pair. The flour GMP content, with excellent correlation to sedimentation value, total contents of HMW glutenin, x- and y-type subunits and many other quality parameters, could be an ideal indicator of breadmaking quality at earlier generations for breeding purpose for its simple procedure and small scale.
