Hypocholesterolemic activity of Crataegus aronia L. (Rosaceae) is therapeutically praised. Its potent antiobesity (P < 0.001, n = 6 - 8) as well as marked triacylglycerol-reducing efficacies (P < 0.001, n = 6 - ...Hypocholesterolemic activity of Crataegus aronia L. (Rosaceae) is therapeutically praised. Its potent antiobesity (P < 0.001, n = 6 - 8) as well as marked triacylglycerol-reducing efficacies (P < 0.001, n = 6 - 8) in 10 weeks-high cholesterol diet (HCD) fed rats are demonstrated. Pancreatic triacylglycerol lipase (PL), α-amylase and α-glucosidase are an interesting pharmacological target for the management of dyslipidemia, atherosclerosis, diabetes and obesity. Comparable to acarbose, acute starch induced postprandial hyperglycaemia as well glycemic excursions in normoglycemic overnight fasting rats was highly significantly (P < 0.001) dampened by C. aronia 100, 200 and 400 mg/Kg b.wt aqueous extracts (AE), but not acute glucose evoked postprandial hyperglycaemia increments, unlike diabetes pharmaco-therapeutics metformin and glipizide. C. aronia aerial parts as well as fruits AEs (0.1 - 10 mg/mL) were identified as in vitro dual inhibitors of α-amylase and α-glucosidase with respective IC50 (mg/mL) of 2.1 ± 0.3 and 3.5 ± 0.7. Still, it lacked on in vitro hindrance of glucose movement, dissimilar to guar gum. Equivalent to orlistat (PL IC50 of 0.1 ± 0.0 μg/mL), C. aronia tested AEs and its purified bioactive phytoconstituents;quercetin and rutin, inhibited highly substantially in a dose dependent trend PL in vitro (n = 3), in an ascending order of obtained PL-IC50 (μg/mL): quercetin;30.1 ± 2.8, rutin;77.3 ± 11.7, C. aronia aerial parts;225.2 ± 33.4 and C. aronia fruits;286.1 ± 37.4. Flavonoid-rich C. aronia, as a functional food and a nutraceutical, modulating gastrointestinal carbohydrate and lipid digestion and absorption, maybe be advocated as an exquisite and potential candidate for combinatorial obesity-diabetes prevention and phytotherapy.展开更多
Recently use of herbal therapies and diet rich in flavonoids and vitamins has augmented significantly to manage minor to modest illnesses. Several anti-hyperlipidemic agents are currently available;however most of the...Recently use of herbal therapies and diet rich in flavonoids and vitamins has augmented significantly to manage minor to modest illnesses. Several anti-hyperlipidemic agents are currently available;however most of them have various unwanted effects. That’s why more people are switching towards safer alternatives, specially derived from plants with fewer side effects. Hence current study was focused to determine the anti-hyperlipidemic potential of Nelumbo nucifera fruit in rabbits fed with high cholesterol diet. This study would surely help in the exploration of a novel agent from the plant source and therefore may recommend the application of traditional medicines in humans. 28 white healthy rabbits of either sex weighing 1000 - 1500 gm were divided into four groups. Control group was given gum tragacanth and 3 groups served as test groups and were given N. nucifera fruit extract 50, 100 and 200 mg/kg for 45 days. Initially animals of all four groups received high cholesterol diet (HCD) 0.125 gm/kg cholesterol in 0.5% corn oil for 30 days. All drugs were given orally. Blood samples were taken thrice from the ear vein of animals, 1st after 24 hours of thirty days of HCD then again after 24 hours of thirty and forty five days dosing of N. nucifera fruit extract. The results showed remarkable cholesterol lowering effects of N. nucifera fruit extract at doses 50, 100 and 200 mg/kg but were particularly dominant at 200 mg/kg at which fruit extract highly significantly reduced TC, TG and LDL-C after 30 and 45 days and increased HDL-C significantly after 30 days and highly significantly after 45 days. Flavonoids, saponins, tannins and procyanidin are significant constituents of N. nucifera fruit which may have contributed in lipid lowering effects of N. nucifera fruit which can contribute in lowering the risk of CVDs and stroke but more preclinical and clinical studies are required to confirm these findings.展开更多
目的:通过比较不同模型建立方法,筛选制备高效、可靠的胆囊胆固醇结石动物模型。方法:将36只5周龄SPF级雄性ICR小鼠随机分为对照组、2%高脂组、1%胆固醇组,每组各12只。对照组采用普通颗粒饲料喂养,2%高脂组采用2%高脂饲料喂养,1%胆固...目的:通过比较不同模型建立方法,筛选制备高效、可靠的胆囊胆固醇结石动物模型。方法:将36只5周龄SPF级雄性ICR小鼠随机分为对照组、2%高脂组、1%胆固醇组,每组各12只。对照组采用普通颗粒饲料喂养,2%高脂组采用2%高脂饲料喂养,1%胆固醇组采用1%胆固醇饲料喂养。喂养16周,记录各组小鼠的死亡数量,计算存活率;观察并记录各组造模成功的小鼠数量及胆囊体积大小、胆汁的浑浊度、透光性等,计算小鼠成石率;ELISA法检测胆囊胆汁总胆固醇(total cholesterol,TC)、总胆汁酸(total bile acids,TBA)、磷脂(phospholipid,PL)及胆囊收缩素(cholecystokinin,CCK)的含量;HE染色观察小鼠胆囊、肝脏病理学变化。结果:对照组小鼠无死亡,胆囊大小正常,胆囊壁结构正常,胆汁澄清透亮,透光性好,胆囊内无沉淀;肝脏细胞排列整齐,结构完整。2%高脂组小鼠死亡1只,胆囊壁肌层变厚,浆膜结构及形态破坏,胆汁呈浑浊状态,透光性差,成石率为66.67%;肝脏细胞结构破坏严重,可见大量脂质空泡,肝脏存在明显的脂肪变性。1%胆固醇组小鼠死亡1只,胆囊体积明显增大,胆囊壁肌层毛糙、增厚,浆膜结构及形态破坏严重,胆汁呈褐色浑浊状态,透光性差,成石率约为83.33%;肝细胞排列紊乱,细胞结构破坏,肝脏出现小的脂质空泡。与对照组比较,2%高脂组和1%胆固醇组胆汁内TC含量升高,胆汁TBA、PL、CCK含量降低;与2%高脂组比较,1%胆固醇组胆汁TC、CCK含量降低,胆汁内TB、PL含量升高,差异均有统计学意义(P<0.01)。结论:1%胆固醇饲料喂养建立胆囊胆固醇结石动物模型效果最好,可作为胆囊胆固醇结石相关研究的备选模型。展开更多
目的应用组织培养方法,了解高胆固醇饲料及活化的巨噬细胞(M(?))对胆囊上皮细胞合成分泌糖蛋白(GP)的影响。方法豚鼠40只,分为普通饲料组(A 组)与高胆固醇饲料组(B 组),饲养1周。培养胆囊组织,测定其合成分泌 GP 的功能;并加入氢化考的...目的应用组织培养方法,了解高胆固醇饲料及活化的巨噬细胞(M(?))对胆囊上皮细胞合成分泌糖蛋白(GP)的影响。方法豚鼠40只,分为普通饲料组(A 组)与高胆固醇饲料组(B 组),饲养1周。培养胆囊组织,测定其合成分泌 GP 的功能;并加入氢化考的松和腹腔 M(?),观测对胆囊组织合成分泌 GP 的影响。结果 1.B 组比 A 组的腹腔 M(?)活力明显升高。2.培养 B 组胆囊组织,释放入培养液中的 GP 含量比 A 组显著增加。3.在 B 组胆囊组织培养液中加入氢化考的松,胆囊组织释放 GP 明显受抑。4.在 A 组胆囊组织培养液中,加入"普 M(?)",胆囊组织释放 GP 的水平与对照水平接近。加入"高 M(?)",浓度较高时胆囊组织分泌 GP 增强;若同时加入氢化考的松,胆囊分泌的 GP 水平明显下降。结论 1.高胆固醇饲料可致豚鼠胆囊组织合成分泌 GP 功能增强。2.高胆固醇饲料能增强豚鼠 M(?)活力,可促进胆囊组织合成分泌 GP。结合动物胆囊结石模型实验的结果推测,可能与豚鼠M(?)因高胆固醇饲料活化,释放细胞因子 TNF、IL-1等有关。展开更多
文摘Hypocholesterolemic activity of Crataegus aronia L. (Rosaceae) is therapeutically praised. Its potent antiobesity (P < 0.001, n = 6 - 8) as well as marked triacylglycerol-reducing efficacies (P < 0.001, n = 6 - 8) in 10 weeks-high cholesterol diet (HCD) fed rats are demonstrated. Pancreatic triacylglycerol lipase (PL), α-amylase and α-glucosidase are an interesting pharmacological target for the management of dyslipidemia, atherosclerosis, diabetes and obesity. Comparable to acarbose, acute starch induced postprandial hyperglycaemia as well glycemic excursions in normoglycemic overnight fasting rats was highly significantly (P < 0.001) dampened by C. aronia 100, 200 and 400 mg/Kg b.wt aqueous extracts (AE), but not acute glucose evoked postprandial hyperglycaemia increments, unlike diabetes pharmaco-therapeutics metformin and glipizide. C. aronia aerial parts as well as fruits AEs (0.1 - 10 mg/mL) were identified as in vitro dual inhibitors of α-amylase and α-glucosidase with respective IC50 (mg/mL) of 2.1 ± 0.3 and 3.5 ± 0.7. Still, it lacked on in vitro hindrance of glucose movement, dissimilar to guar gum. Equivalent to orlistat (PL IC50 of 0.1 ± 0.0 μg/mL), C. aronia tested AEs and its purified bioactive phytoconstituents;quercetin and rutin, inhibited highly substantially in a dose dependent trend PL in vitro (n = 3), in an ascending order of obtained PL-IC50 (μg/mL): quercetin;30.1 ± 2.8, rutin;77.3 ± 11.7, C. aronia aerial parts;225.2 ± 33.4 and C. aronia fruits;286.1 ± 37.4. Flavonoid-rich C. aronia, as a functional food and a nutraceutical, modulating gastrointestinal carbohydrate and lipid digestion and absorption, maybe be advocated as an exquisite and potential candidate for combinatorial obesity-diabetes prevention and phytotherapy.
文摘Recently use of herbal therapies and diet rich in flavonoids and vitamins has augmented significantly to manage minor to modest illnesses. Several anti-hyperlipidemic agents are currently available;however most of them have various unwanted effects. That’s why more people are switching towards safer alternatives, specially derived from plants with fewer side effects. Hence current study was focused to determine the anti-hyperlipidemic potential of Nelumbo nucifera fruit in rabbits fed with high cholesterol diet. This study would surely help in the exploration of a novel agent from the plant source and therefore may recommend the application of traditional medicines in humans. 28 white healthy rabbits of either sex weighing 1000 - 1500 gm were divided into four groups. Control group was given gum tragacanth and 3 groups served as test groups and were given N. nucifera fruit extract 50, 100 and 200 mg/kg for 45 days. Initially animals of all four groups received high cholesterol diet (HCD) 0.125 gm/kg cholesterol in 0.5% corn oil for 30 days. All drugs were given orally. Blood samples were taken thrice from the ear vein of animals, 1st after 24 hours of thirty days of HCD then again after 24 hours of thirty and forty five days dosing of N. nucifera fruit extract. The results showed remarkable cholesterol lowering effects of N. nucifera fruit extract at doses 50, 100 and 200 mg/kg but were particularly dominant at 200 mg/kg at which fruit extract highly significantly reduced TC, TG and LDL-C after 30 and 45 days and increased HDL-C significantly after 30 days and highly significantly after 45 days. Flavonoids, saponins, tannins and procyanidin are significant constituents of N. nucifera fruit which may have contributed in lipid lowering effects of N. nucifera fruit which can contribute in lowering the risk of CVDs and stroke but more preclinical and clinical studies are required to confirm these findings.
文摘目的:通过比较不同模型建立方法,筛选制备高效、可靠的胆囊胆固醇结石动物模型。方法:将36只5周龄SPF级雄性ICR小鼠随机分为对照组、2%高脂组、1%胆固醇组,每组各12只。对照组采用普通颗粒饲料喂养,2%高脂组采用2%高脂饲料喂养,1%胆固醇组采用1%胆固醇饲料喂养。喂养16周,记录各组小鼠的死亡数量,计算存活率;观察并记录各组造模成功的小鼠数量及胆囊体积大小、胆汁的浑浊度、透光性等,计算小鼠成石率;ELISA法检测胆囊胆汁总胆固醇(total cholesterol,TC)、总胆汁酸(total bile acids,TBA)、磷脂(phospholipid,PL)及胆囊收缩素(cholecystokinin,CCK)的含量;HE染色观察小鼠胆囊、肝脏病理学变化。结果:对照组小鼠无死亡,胆囊大小正常,胆囊壁结构正常,胆汁澄清透亮,透光性好,胆囊内无沉淀;肝脏细胞排列整齐,结构完整。2%高脂组小鼠死亡1只,胆囊壁肌层变厚,浆膜结构及形态破坏,胆汁呈浑浊状态,透光性差,成石率为66.67%;肝脏细胞结构破坏严重,可见大量脂质空泡,肝脏存在明显的脂肪变性。1%胆固醇组小鼠死亡1只,胆囊体积明显增大,胆囊壁肌层毛糙、增厚,浆膜结构及形态破坏严重,胆汁呈褐色浑浊状态,透光性差,成石率约为83.33%;肝细胞排列紊乱,细胞结构破坏,肝脏出现小的脂质空泡。与对照组比较,2%高脂组和1%胆固醇组胆汁内TC含量升高,胆汁TBA、PL、CCK含量降低;与2%高脂组比较,1%胆固醇组胆汁TC、CCK含量降低,胆汁内TB、PL含量升高,差异均有统计学意义(P<0.01)。结论:1%胆固醇饲料喂养建立胆囊胆固醇结石动物模型效果最好,可作为胆囊胆固醇结石相关研究的备选模型。
文摘目的应用组织培养方法,了解高胆固醇饲料及活化的巨噬细胞(M(?))对胆囊上皮细胞合成分泌糖蛋白(GP)的影响。方法豚鼠40只,分为普通饲料组(A 组)与高胆固醇饲料组(B 组),饲养1周。培养胆囊组织,测定其合成分泌 GP 的功能;并加入氢化考的松和腹腔 M(?),观测对胆囊组织合成分泌 GP 的影响。结果 1.B 组比 A 组的腹腔 M(?)活力明显升高。2.培养 B 组胆囊组织,释放入培养液中的 GP 含量比 A 组显著增加。3.在 B 组胆囊组织培养液中加入氢化考的松,胆囊组织释放 GP 明显受抑。4.在 A 组胆囊组织培养液中,加入"普 M(?)",胆囊组织释放 GP 的水平与对照水平接近。加入"高 M(?)",浓度较高时胆囊组织分泌 GP 增强;若同时加入氢化考的松,胆囊分泌的 GP 水平明显下降。结论 1.高胆固醇饲料可致豚鼠胆囊组织合成分泌 GP 功能增强。2.高胆固醇饲料能增强豚鼠 M(?)活力,可促进胆囊组织合成分泌 GP。结合动物胆囊结石模型实验的结果推测,可能与豚鼠M(?)因高胆固醇饲料活化,释放细胞因子 TNF、IL-1等有关。
