AIM To investigate the role of apolipoprotein E (apoE) polymorphism in the lithogenesis of gallstone and the hereditary pathogenesis of the disease.METHODS Polymerase chain reaction (PCR)was used to study apoE phenoty...AIM To investigate the role of apolipoprotein E (apoE) polymorphism in the lithogenesis of gallstone and the hereditary pathogenesis of the disease.METHODS Polymerase chain reaction (PCR)was used to study apoE phenotypes and allelefrequencies in patients with gallstones and control, and the fasting serum lipids of subjectswere also measured by enzymatic methods.RESULTS The levels of triglyceride (TG) andvery low density lipoprotein cholesterol (VLDLC) were much higher in Ez/, patients than that inE,/, control. E,/, patients were accompanied withremarkably low levels of high density lipoproteincholesterol (HDLC) and its subforms. But in E,/#patients there were only slight changes in levelsof VLDLC and low density lipoprotein cholesterol (LDL--C).CONCLUSION Different apoE phenotype patientswith gallstones have different cheracteristics ofdyslipidemia and the average level of serum lipids in patients with gallstones are higher thansubjects without gallstones in the same apoEgene phenotype. EZ allele is possibly one of thedangerous factors in the lithogenesis of cholecystolithiasis.展开更多
High Available System Software HHA is developed by Institution of Command and Technology of Equipment. The paper describes the management functions based on servers of HHA system ,and analyses the main techniques of i...High Available System Software HHA is developed by Institution of Command and Technology of Equipment. The paper describes the management functions based on servers of HHA system ,and analyses the main techniques of implementing those functions.展开更多
Abstract:Objective To study Sma Ⅰ, Hha Ⅰ, Msp Ⅰ and Rsa Ⅰ restriction fragment length polymorphisms in vWF gene of Chinese population. Methods The vWF gene from 52 unrelated individuals of Han Nationality, 44 of...Abstract:Objective To study Sma Ⅰ, Hha Ⅰ, Msp Ⅰ and Rsa Ⅰ restriction fragment length polymorphisms in vWF gene of Chinese population. Methods The vWF gene from 52 unrelated individuals of Han Nationality, 44 of Yi tribe, 42 of Dai tribe was analyzed by polymerase chain reaction and digestion of the endonucleases of Sma Ⅰ, Hha Ⅰ, Msp Ⅰ and Rsa Ⅰ.Results The allele frequencies were 0.38∶ 0.62 for Sma Ⅰ+∶ Sma Ⅰ- and 0.38∶0.62 for Hha Ⅰ+∶ Hha Ⅰ-; 0.20∶ 0.80 for Msp Ⅰ+∶ Msp Ⅰ-; 0.93∶ 0.07 for Rsa Ⅰ+∶ Rsa Ⅰ- in Chinese Han population. The theoretical heterozygous rates were 0.46, 0.46, 0.36 and 0.13 of these polymorphisms, respectively. The allele frequencies were 0.43∶ 0.57 for Sma Ⅰ+∶ Sma Ⅰ- and 0.43∶0.57 for Hha Ⅰ+∶ Hha Ⅰ-, 0.26∶0.74 for Msp Ⅰ+∶ Msp- in Yi tribe and 0.36∶0.64 for Sma Ⅰ+∶ Sma Ⅰ- and Hha Ⅰ+∶ Hha Ⅰ-, 0.17∶ 0.83 for Msp Ⅰ+∶ Msp Ⅰ- in Dai tribe. The theoretical rates were 0.49 for Sma Ⅰ and Hha Ⅰ and 0.38 for Msp Ⅰ in Yi tribe; and 0.45 for Sma Ⅰ and Hha Ⅰ, 0.28 for Msp Ⅰ in Dai tribe, respectively.Conclusions The allele frequencies and theoretical rates of these polymorphisms in vWF gene are different between Chinese populations and Caucasians. They should be useful for the carrier detection and prenatal diagnosis in vWD families of Chinese.展开更多
基金Project supported by National Natural Science Foundation of China,No.39670709.
文摘AIM To investigate the role of apolipoprotein E (apoE) polymorphism in the lithogenesis of gallstone and the hereditary pathogenesis of the disease.METHODS Polymerase chain reaction (PCR)was used to study apoE phenotypes and allelefrequencies in patients with gallstones and control, and the fasting serum lipids of subjectswere also measured by enzymatic methods.RESULTS The levels of triglyceride (TG) andvery low density lipoprotein cholesterol (VLDLC) were much higher in Ez/, patients than that inE,/, control. E,/, patients were accompanied withremarkably low levels of high density lipoproteincholesterol (HDLC) and its subforms. But in E,/#patients there were only slight changes in levelsof VLDLC and low density lipoprotein cholesterol (LDL--C).CONCLUSION Different apoE phenotype patientswith gallstones have different cheracteristics ofdyslipidemia and the average level of serum lipids in patients with gallstones are higher thansubjects without gallstones in the same apoEgene phenotype. EZ allele is possibly one of thedangerous factors in the lithogenesis of cholecystolithiasis.
文摘High Available System Software HHA is developed by Institution of Command and Technology of Equipment. The paper describes the management functions based on servers of HHA system ,and analyses the main techniques of implementing those functions.
文摘Abstract:Objective To study Sma Ⅰ, Hha Ⅰ, Msp Ⅰ and Rsa Ⅰ restriction fragment length polymorphisms in vWF gene of Chinese population. Methods The vWF gene from 52 unrelated individuals of Han Nationality, 44 of Yi tribe, 42 of Dai tribe was analyzed by polymerase chain reaction and digestion of the endonucleases of Sma Ⅰ, Hha Ⅰ, Msp Ⅰ and Rsa Ⅰ.Results The allele frequencies were 0.38∶ 0.62 for Sma Ⅰ+∶ Sma Ⅰ- and 0.38∶0.62 for Hha Ⅰ+∶ Hha Ⅰ-; 0.20∶ 0.80 for Msp Ⅰ+∶ Msp Ⅰ-; 0.93∶ 0.07 for Rsa Ⅰ+∶ Rsa Ⅰ- in Chinese Han population. The theoretical heterozygous rates were 0.46, 0.46, 0.36 and 0.13 of these polymorphisms, respectively. The allele frequencies were 0.43∶ 0.57 for Sma Ⅰ+∶ Sma Ⅰ- and 0.43∶0.57 for Hha Ⅰ+∶ Hha Ⅰ-, 0.26∶0.74 for Msp Ⅰ+∶ Msp- in Yi tribe and 0.36∶0.64 for Sma Ⅰ+∶ Sma Ⅰ- and Hha Ⅰ+∶ Hha Ⅰ-, 0.17∶ 0.83 for Msp Ⅰ+∶ Msp Ⅰ- in Dai tribe. The theoretical rates were 0.49 for Sma Ⅰ and Hha Ⅰ and 0.38 for Msp Ⅰ in Yi tribe; and 0.45 for Sma Ⅰ and Hha Ⅰ, 0.28 for Msp Ⅰ in Dai tribe, respectively.Conclusions The allele frequencies and theoretical rates of these polymorphisms in vWF gene are different between Chinese populations and Caucasians. They should be useful for the carrier detection and prenatal diagnosis in vWD families of Chinese.
