目的:建立和验证人孕烷X受体(human pregnant X receptor,hPXR)介导的CYP3A4、MDR1药物诱导剂的体外筛选体系,考察豆腐果苷对hPXR介导的CYP3A4、MDR1的转录调节作用。方法:利用构建的双荧光素酶报告基因系统,将表达载体和报告载体共转染...目的:建立和验证人孕烷X受体(human pregnant X receptor,hPXR)介导的CYP3A4、MDR1药物诱导剂的体外筛选体系,考察豆腐果苷对hPXR介导的CYP3A4、MDR1的转录调节作用。方法:利用构建的双荧光素酶报告基因系统,将表达载体和报告载体共转染HepG2细胞,以10μmol/L利福平为阳性对照,用不同浓度(0.004、0.04、0.4μmol/L)豆腐果苷处理48h后裂解细胞进行双荧光素酶活性检测。结果:不同浓度的豆腐果苷均不能通过激活hPXR来介导CYP3A4和MDR1表达上调,各浓度处理组的双荧光素酶比活性值与DMSO溶媒组差异无统计学意义(P>0.05)。结论:成功构建了hPXR介导的CYP3A4和MDR1药物诱导剂的体外筛选体系,并发现豆腐果苷不能通过激活hPXR介导CYP3A4和MDR1的表达上调。展开更多
The orthogonal design was used to optimize the formulation of helicid orally disintegrating tabletsprepared by direct powder compression method. The optimized formulation was as follows: weight ratio ofpolyvinylpolypy...The orthogonal design was used to optimize the formulation of helicid orally disintegrating tabletsprepared by direct powder compression method. The optimized formulation was as follows: weight ratio ofpolyvinylpolypyrrolidone and low-substituted hydroxypropylcellulose 1∶1; the amount of NaHCO3-citric acid(1∶1)14% and microcrystalline cellulose 10%. The prepared tablets disintegrated completely within 30s, dissolved in 2min andtasted fine.展开更多
文摘目的:建立和验证人孕烷X受体(human pregnant X receptor,hPXR)介导的CYP3A4、MDR1药物诱导剂的体外筛选体系,考察豆腐果苷对hPXR介导的CYP3A4、MDR1的转录调节作用。方法:利用构建的双荧光素酶报告基因系统,将表达载体和报告载体共转染HepG2细胞,以10μmol/L利福平为阳性对照,用不同浓度(0.004、0.04、0.4μmol/L)豆腐果苷处理48h后裂解细胞进行双荧光素酶活性检测。结果:不同浓度的豆腐果苷均不能通过激活hPXR来介导CYP3A4和MDR1表达上调,各浓度处理组的双荧光素酶比活性值与DMSO溶媒组差异无统计学意义(P>0.05)。结论:成功构建了hPXR介导的CYP3A4和MDR1药物诱导剂的体外筛选体系,并发现豆腐果苷不能通过激活hPXR介导CYP3A4和MDR1的表达上调。
文摘The orthogonal design was used to optimize the formulation of helicid orally disintegrating tabletsprepared by direct powder compression method. The optimized formulation was as follows: weight ratio ofpolyvinylpolypyrrolidone and low-substituted hydroxypropylcellulose 1∶1; the amount of NaHCO3-citric acid(1∶1)14% and microcrystalline cellulose 10%. The prepared tablets disintegrated completely within 30s, dissolved in 2min andtasted fine.
