Aim To study the chemical constituents of Euphorbia ebracteolata Hayata. Methods Column chromatography was used in the isolation procedure, while the structures of isolated compounds were elucidated by spectral data...Aim To study the chemical constituents of Euphorbia ebracteolata Hayata. Methods Column chromatography was used in the isolation procedure, while the structures of isolated compounds were elucidated by spectral data. Results Six compounds were isolated and their structures were identified as baccatin (1), 3-acetyl-β-amyrin (2), 3,3′-diacetyl-4,4′-dimethoxy- 2,2′,6,6′-tetrah ydroxy diphenylmethane (3), 2,4-dihydroxy-6-methoxy-3-methyl acetophenone (4), β-sitosterol (5), and daucosterol (6). Conclusion Baccatin was obtained from Euphorbia ebracteolata for the first time.展开更多
[Objective] This research aimed to study the taxonomic status of a potential energy plant, E. kansuensis Prokh (Euphorbiaceae). [Method] Fresh plants collected after field survey and specimens were observed and compar...[Objective] This research aimed to study the taxonomic status of a potential energy plant, E. kansuensis Prokh (Euphorbiaceae). [Method] Fresh plants collected after field survey and specimens were observed and compared to study the morphological characteristics and distribution of E. kansuensis Prokh. and E. ebraceolata Hayata in Subgen. Esula distributed in Jiangsu Province. [Result] There was no obvious morphological distinction but some overlaps between E. kansuensis Prokh. in Flora of China and E. ebraceolata Hayata in Flora of Jiangsu based on indumentum and root characteristics, therefore the standpoint that E. ebraceolata Hayata was a wrong name for E. kansuensis Prokh. in Flora of China proved to be reasonable. However, the description of E. kansuensis Prokh. should be modified as follows: indumentums, present or absent; roots, fusiform to terete; glands, oblong kidney-shaped. [Conclusion] This study is of great significance for the taxonomic identification of E. kansuensis Prokh.展开更多
Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components ...Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components alter bone metabolism. Methods： The effects of low and high doses of D. formosana water extracts （DFW, 50 and 200 mg/（kg·d）） and （-）-epicatechin-3-O-D-allopyranoside isolates of DFW （ECAP, 0.06 and 2.5 mg/（kg·d）） on protein lev- els of important indicators of osteoclast and osteoblast activities were examined. Ovariectomized mice were used as a model of osteoporosis. Bone morphogenetic protein 2 （BMP-2）, collagen 1 （COL-1）, alka- line phosphatase （ALP）, runt-related transcription factor 2 （RUNX2）, osteocalcin （OCN）, and osteopontin （OPN） were analyzed using western blotting of femur tissue. Interleukin （IL）-1β and tumor necrosis fac- tor （TNF）-a were measured in the serum, and hematoxylin and eosin staining were used to assess bone morphology. Results： Treatment with DFW or ECAP significantly induced BMP-2, COL-l, ALP, RUNX2, OCN, and OPN protein expression, indicating stimulation of osteogenesis. The treatments also reduced IL-1β and TNF- expression, indicating inhibition of osteoclastogenesis. In histological examinations, mice treated with DFW or ECAP had more bone trabeculae. The results demonstrate that DFW and ECAP inhibit osteo- clast differentiation and promote osteoblast differentiation, and effectively ameliorate ovariectomy （OVX）- induced osteoporosis. Conclusion： ECAP is a major component of DFW, which may have therapeutic potential for the treatment of diseases associated with excessive osteoclastic and insufficient osteogenic activity. ECAP may not be the specific active molecule because it is unstable in the stomach. However, the major active molecule needs to be confirmed using a relevant pharmacological model.展开更多
文摘Aim To study the chemical constituents of Euphorbia ebracteolata Hayata. Methods Column chromatography was used in the isolation procedure, while the structures of isolated compounds were elucidated by spectral data. Results Six compounds were isolated and their structures were identified as baccatin (1), 3-acetyl-β-amyrin (2), 3,3′-diacetyl-4,4′-dimethoxy- 2,2′,6,6′-tetrah ydroxy diphenylmethane (3), 2,4-dihydroxy-6-methoxy-3-methyl acetophenone (4), β-sitosterol (5), and daucosterol (6). Conclusion Baccatin was obtained from Euphorbia ebracteolata for the first time.
文摘[Objective] This research aimed to study the taxonomic status of a potential energy plant, E. kansuensis Prokh (Euphorbiaceae). [Method] Fresh plants collected after field survey and specimens were observed and compared to study the morphological characteristics and distribution of E. kansuensis Prokh. and E. ebraceolata Hayata in Subgen. Esula distributed in Jiangsu Province. [Result] There was no obvious morphological distinction but some overlaps between E. kansuensis Prokh. in Flora of China and E. ebraceolata Hayata in Flora of Jiangsu based on indumentum and root characteristics, therefore the standpoint that E. ebraceolata Hayata was a wrong name for E. kansuensis Prokh. in Flora of China proved to be reasonable. However, the description of E. kansuensis Prokh. should be modified as follows: indumentums, present or absent; roots, fusiform to terete; glands, oblong kidney-shaped. [Conclusion] This study is of great significance for the taxonomic identification of E. kansuensis Prokh.
基金Yupintang Traditional Chinese Medicine Foundation for continuously supporting me through my research
文摘Objective： The Taiwan Residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components alter bone metabolism. Methods： The effects of low and high doses of D. formosana water extracts （DFW, 50 and 200 mg/（kg·d）） and （-）-epicatechin-3-O-D-allopyranoside isolates of DFW （ECAP, 0.06 and 2.5 mg/（kg·d）） on protein lev- els of important indicators of osteoclast and osteoblast activities were examined. Ovariectomized mice were used as a model of osteoporosis. Bone morphogenetic protein 2 （BMP-2）, collagen 1 （COL-1）, alka- line phosphatase （ALP）, runt-related transcription factor 2 （RUNX2）, osteocalcin （OCN）, and osteopontin （OPN） were analyzed using western blotting of femur tissue. Interleukin （IL）-1β and tumor necrosis fac- tor （TNF）-a were measured in the serum, and hematoxylin and eosin staining were used to assess bone morphology. Results： Treatment with DFW or ECAP significantly induced BMP-2, COL-l, ALP, RUNX2, OCN, and OPN protein expression, indicating stimulation of osteogenesis. The treatments also reduced IL-1β and TNF- expression, indicating inhibition of osteoclastogenesis. In histological examinations, mice treated with DFW or ECAP had more bone trabeculae. The results demonstrate that DFW and ECAP inhibit osteo- clast differentiation and promote osteoblast differentiation, and effectively ameliorate ovariectomy （OVX）- induced osteoporosis. Conclusion： ECAP is a major component of DFW, which may have therapeutic potential for the treatment of diseases associated with excessive osteoclastic and insufficient osteogenic activity. ECAP may not be the specific active molecule because it is unstable in the stomach. However, the major active molecule needs to be confirmed using a relevant pharmacological model.
