Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As a...Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes （HFDRGs） in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxcl3 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFP- Hoxc13. The result suggested that overexpression ofHoxcl3 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.展开更多
Objective:To analyze the dynamic expression of Wnt family member 5A(Wingless-type MMTV integration Wnt site family,member 5a)in murine hair cycle and its inhibitory effects on follicle in vivo.Methods:Situ hybridizati...Objective:To analyze the dynamic expression of Wnt family member 5A(Wingless-type MMTV integration Wnt site family,member 5a)in murine hair cycle and its inhibitory effects on follicle in vivo.Methods:Situ hybridization in full-thickness skin was used to observe the change of mouse protein expression in different growth stages,and Ad-Wnt5a was injected after defeathering to observe the hair follicle growth in vivo.Results:The Wnt5a mRNA was expressed at birth,and was firstly increased then decreased along with the progress of the hair cycle.It reached the peak in advanced stage of growth cycle(P<0.05).Rhoa andβ-catenin expression levels were significantly decreased in three groups.Rac2 expression was significantly up-regulated,and the expression level of Wnt5a,Shh and Frizzled2 was increased,but less significantly than group 2.Conclusions:The expression of Wnt5a mRNA is consistent with change of murine follicle cycle,and has obvious inhibitory effects on the growth of hair follicle in vivo,indicating that it is antagonistic to Wnts pathway and interferes the growth of follicle together.展开更多
基金the National Natural Science Foundation of China (30960246)the Key Project of National Science and Technology Pillar Program of China (2011BAD28B05)+3 种基金the National High Technology Research and Development Program of China (2007AA10Z151)the Specialized Research Fund for the Doctoral Program of Higher Education(20091515120010)the Inner Mongolia Natural Science Foundation, China (20080404ZD04)the China Agriculture Research System (CARS-40)
文摘Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes （HFDRGs） in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxcl3 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFP- Hoxc13. The result suggested that overexpression ofHoxcl3 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.
基金supported by Zhejiang University Medical Key Funds(No201028382)
文摘Objective:To analyze the dynamic expression of Wnt family member 5A(Wingless-type MMTV integration Wnt site family,member 5a)in murine hair cycle and its inhibitory effects on follicle in vivo.Methods:Situ hybridization in full-thickness skin was used to observe the change of mouse protein expression in different growth stages,and Ad-Wnt5a was injected after defeathering to observe the hair follicle growth in vivo.Results:The Wnt5a mRNA was expressed at birth,and was firstly increased then decreased along with the progress of the hair cycle.It reached the peak in advanced stage of growth cycle(P<0.05).Rhoa andβ-catenin expression levels were significantly decreased in three groups.Rac2 expression was significantly up-regulated,and the expression level of Wnt5a,Shh and Frizzled2 was increased,but less significantly than group 2.Conclusions:The expression of Wnt5a mRNA is consistent with change of murine follicle cycle,and has obvious inhibitory effects on the growth of hair follicle in vivo,indicating that it is antagonistic to Wnts pathway and interferes the growth of follicle together.
