AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells（SGC-7901,MKN-45,MKN-28）withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The ...AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells（SGC-7901,MKN-45,MKN-28）withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As<sub>2</sub>O<sub>3</sub> and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As<sub>2</sub>O<sub>3</sub> were investigatedby TUNEL method and flow cytometry.RESULTS As<sub>2</sub>O<sub>3</sub> and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC<sub>50</sub>ofAs<sub>2</sub>O<sub>3</sub> on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC<sub>50</sub>of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G<sub>0</sub>/G<sub>1</sub> phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As<sub>2</sub>O<sub>3</sub>.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As<sub>2</sub>O<sub>3</sub> on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G<sub>1</sub> phase,and blocked G<sub>2</sub>/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG<sub>1</sub> phase and arres展开更多
Background Superficial bladder cancer accounts for 60%-70% of all bladder cancer cases in China, when treatment consists of only transurethral resection of the bladder tumor （TUR-BT）, recurrence and progresses in th...Background Superficial bladder cancer accounts for 60%-70% of all bladder cancer cases in China, when treatment consists of only transurethral resection of the bladder tumor （TUR-BT）, recurrence and progresses in the bladder are observed in some patients. There are numerous reports of trials of intravesical instillation of anticancer agents with the objective of lowering this recurrence rate. The aim of this study was to compare the prophylactic efficacy and safety of epirubicin （EPI）, pirarubicin （THP） and hydroxycamptothecin （HCPT） in superficial bladder cancer.Methods This study enrolled a total of 189 patients who had been diagnosed with superficial bladder cancer during the period from 2004 through 2007 at Beijing Friendship Hospital. All patients were randomly allocated to one of three treatment groups. Patients in group A received 29 doses of EPI 30 mg/30 ml, patients in group B received 29 doses of THP 30 mg/30 ml, and patients in group C received 29 doses of HCPT 30 mg/30 ml, over a period of 24 months.Results The recurrence-free rate in the 2 anthracycline treatment groups （A and B） were significantly better than that of the HCPT treatment group. In the safety evaluation, the incidences of pollakiuria, pain on urination, dysuria, hematuria,and contracted bladder were not significantly different between groups A and B, but some were significantly higher in groups A and B than that in group C.Conclusion The efficacy of EPI and THP was significantly better than HCPT in the prevention of bladder cancer recurrence.展开更多
基金the Natural Science Foundation of Committee of Science and Technology of Shanghai Municipality(№964119035)
文摘AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells（SGC-7901,MKN-45,MKN-28）withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As<sub>2</sub>O<sub>3</sub> and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As<sub>2</sub>O<sub>3</sub> were investigatedby TUNEL method and flow cytometry.RESULTS As<sub>2</sub>O<sub>3</sub> and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC<sub>50</sub>ofAs<sub>2</sub>O<sub>3</sub> on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC<sub>50</sub>of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G<sub>0</sub>/G<sub>1</sub> phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As<sub>2</sub>O<sub>3</sub>.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As<sub>2</sub>O<sub>3</sub> on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G<sub>1</sub> phase,and blocked G<sub>2</sub>/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG<sub>1</sub> phase and arres
文摘目的探讨吡柔比星(pirarubicin,THP)、羟基喜树碱(hydroxycamptothecin,HCPT)、丝裂霉素C(mitomycin C,MMC)三种不同药物膀胱灌注化疗,预防浅表性膀胱癌术后复发的疗效和安全性。方法对130例行尿道膀胱癌电切术(transurethralresection of bladder tumor,TUR-Bt)后的浅表性膀胱癌患者,随机分为3组:Ⅰ组THP(48例)、Ⅱ组HCPT(41例)、Ⅲ组MMC(41例),分别于术后1-2周开始规律膀胱灌注,比较3组肿瘤复发率。结果随访3-24个月,平均14个月;Ⅰ、Ⅱ和Ⅲ组2年复发率分别为16.67%(8/48)、17.07%(7/41)和36.59%(15/41);3组比较Ⅰ、Ⅱ组无统计学差异(P>0.05),Ⅰ、Ⅱ组复发率均低于Ⅲ组,Ⅰ、Ⅱ组与Ⅲ组比较差异显著(P<0.05)。结论THP、HCPT膀胱灌注预防浅表性膀胱癌术后复发疗效满意,副作用轻,耐受性良好。
文摘Background Superficial bladder cancer accounts for 60%-70% of all bladder cancer cases in China, when treatment consists of only transurethral resection of the bladder tumor （TUR-BT）, recurrence and progresses in the bladder are observed in some patients. There are numerous reports of trials of intravesical instillation of anticancer agents with the objective of lowering this recurrence rate. The aim of this study was to compare the prophylactic efficacy and safety of epirubicin （EPI）, pirarubicin （THP） and hydroxycamptothecin （HCPT） in superficial bladder cancer.Methods This study enrolled a total of 189 patients who had been diagnosed with superficial bladder cancer during the period from 2004 through 2007 at Beijing Friendship Hospital. All patients were randomly allocated to one of three treatment groups. Patients in group A received 29 doses of EPI 30 mg/30 ml, patients in group B received 29 doses of THP 30 mg/30 ml, and patients in group C received 29 doses of HCPT 30 mg/30 ml, over a period of 24 months.Results The recurrence-free rate in the 2 anthracycline treatment groups （A and B） were significantly better than that of the HCPT treatment group. In the safety evaluation, the incidences of pollakiuria, pain on urination, dysuria, hematuria,and contracted bladder were not significantly different between groups A and B, but some were significantly higher in groups A and B than that in group C.Conclusion The efficacy of EPI and THP was significantly better than HCPT in the prevention of bladder cancer recurrence.
