The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait,meat quality and lipid metabolism of finishing Huan Jiang mini-pigs.Betaine called trimethylglycine is a methyl ...The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait,meat quality and lipid metabolism of finishing Huan Jiang mini-pigs.Betaine called trimethylglycine is a methyl derivative of glycine,but few researches were conducted to compare the impact of dietary betaine and glycine on pigs.One hundred and forty-four Huan Jiang mini-pigs(body weight=10.55±0.15 kg;70 d)were randomly divided to 3 treatment groups(basal diet,glycine or betaine).Results indicated that dietary betaine increased the average daily gain(ADG)and final weight(P<0.05).Dietary glycine or betaine markedly reduced average backfat thickness(P<0.05)and heightened lean percentage(P<0.01)compared to the control group.Moreover,in comparison with the control group,betaine significantly improved the redness(a*)and tenderness(shear force)of the longissimus dorsi(LD)muscle(P<0.05),whereas glycine only raised the value of a*of the LD muscle(P<0.05).These results showed that diet supplemented with 0.25%betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs,and the effect of betaine was superior to that of glycine.展开更多
Hyperspectral data are an important source for monitoring soil salt content on a large scale. However, in previous studies, barriers such as interference due to the presence of vegetation restricted the precision of m...Hyperspectral data are an important source for monitoring soil salt content on a large scale. However, in previous studies, barriers such as interference due to the presence of vegetation restricted the precision of mapping soil salt content. This study tested a new method for predicting soil salt content with improved precision by using Chinese hyperspectral data, Huan Jing-Hyper Spectral Imager(HJ-HSI), in the coastal area of Rudong County, Eastern China. The vegetation-covered area and coastal bare flat area were distinguished by using the normalized differential vegetation index at the band length of 705 nm(NDVI705). The soil salt content of each area was predicted by various algorithms. A Normal Soil Salt Content Response Index(NSSRI) was constructed from continuum-removed reflectance(CR-reflectance) at wavelengths of 908.95 nm and 687.41 nm to predict the soil salt content in the coastal bare flat area(NDVI705 < 0.2). The soil adjusted salinity index(SAVI) was applied to predict the soil salt content in the vegetation-covered area(NDVI705 ≥ 0.2). The results demonstrate that 1) the new method significantly improves the accuracy of soil salt content mapping(R2 = 0.6396, RMSE = 0.3591), and 2) HJ-HSI data can be used to map soil salt content precisely and are suitable for monitoring soil salt content on a large scale.展开更多
Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustio...Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustion method and Rubrospinal Tract(RST)transection were combined to establish arat model of SCI with pathopattern of QDBS in TCM.Macroscopic representation,including the body weight,food intake and tongue scores,was recorded.Behavioristics was scored with spontaneous vertical exploration test.Hemorrheology was analyzed using hemorheological analyzer and morphology of spinal cord was observed.The feasibility of the model was confirmed on the basis of the reversion caused by administration of Bu Yang Huan Wu Decoction(BYHWD).Results The findings in the QDBS group were consistent with the symptoms of QDBS syndrome,including reduced body weight,food intake,and forelimb utilization rate and increased tongue scores.However,the corresponding findings in the BYHWD group were superior to those in the QDBS group after35d(P<0.05).In comparison with the control group,the QDBS group showed higher whole blood viscosity and hematocrit values(P<0.05)and a lower red blood cell(RBC)deformability index(P<0.05),while the corresponding findings in the BYHWD group were close to those in the control group.An eminence or induration appeared in the injured spinal cord,which was suspected to be vacuoles or a scar.Hematoxylin-eosin staining showed that the R/T ratio in the BYHWD group was higher than that in the QDBS group(P<0.05).Conclusion Swimming exhaustion method combined with RST transection operation can successfully establish a rat model of SCI with pathopattern of QDBS.展开更多
To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlus...To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and b展开更多
With in vitro spin labeling electron spin resonance (ESR) spectroscopy, we have studied the effects of Bu Yang Huan Wu (BYHW) decoction and its effective constituents such as astragaloside IV ferulic acid, chua...With in vitro spin labeling electron spin resonance (ESR) spectroscopy, we have studied the effects of Bu Yang Huan Wu (BYHW) decoction and its effective constituents such as astragaloside IV ferulic acid, chuanxiongzine, rutin, chlorogenic acid, 9,10 dimethoxy pterocarpane 7 O β D glucoside, calycosin, formononetin, calycosin 7 O glucoside, paeoniflorin, paeonal and quercein on the cell membrane fluidity of a rat brain which was modeled after the dual cervical arteries were intercepted and released for realizing an ischemia reperfusion injury which was selected as a brain stroke model. Our results indicated that the cell membrane fluidity in the model group decreased approximately 8% compared with the control group, and after brain cells were incubatied with species, the membrane fluidity could be recovered closely to the control level depending on the BYHW decoction and its different constituents. As the membrane fluidity is a very sensitive biological index which reflectsd the cell status, our method will be useful to study the molecular mechanism of tradition Chinese medicine (TCM) and its combination recipe.展开更多
Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar ra...Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.展开更多
The author uses the cooperative principle to analyze the conversational implicatures in the Legend of Zhen Huan by examining how the maxims of the cooperative principle are violated.By writing this article, the author...The author uses the cooperative principle to analyze the conversational implicatures in the Legend of Zhen Huan by examining how the maxims of the cooperative principle are violated.By writing this article, the author hopes to let the audience have a further appreciation of this work and enjoy more about the charms of language.展开更多
Stroke is the leading cause of human disability with limited effective drugs availability. The disruption of multiple signaling pathways in stroke makes developing new drugs be difficult. Traditional Chinese medicine(...Stroke is the leading cause of human disability with limited effective drugs availability. The disruption of multiple signaling pathways in stroke makes developing new drugs be difficult. Traditional Chinese medicine(TCM) has its unique advantage in targeting multiple signaling pathways with multiple components. Many TCM formulas have the potential for neuroprotection and neurogenesis, but well-designed clinical trials are insufficient and underlying mechanisms unclear. Herein, we introduce the commonly used TCM formulas for stroke treatment and selectively introduce two classic formulas, An Gong Niu Huang(AGNH) pill and Bu Yang Huan Wu Decoction(BYHWD) for neuroprotection and neurogenesis, respectively. Current scientific evidence and clinical trials indicate AGNH pill could be an effective neuroprotective formula as adjunct therapy with relative safety. On the other hand, as a representative TCM formula for post stroke disability, BYHWD could improve the neurological outcome with its neuroprotective and neurogenic effects. The underlying mechanisms could be attributed to the modulation of multiple molecular targets with its multiple components. In conclusion, TCM formulas could be potential adjunct therapies for stroke treatment. The clinical efficacies and molecular mechanisms of promoting neuroprotection and neurogenesis remain to be further studies.展开更多
Recently, Hitachi Metals San Huan Magnetic Materials (Nantong) Co., Ltd. jointly established by Beijing Zhongke Sanhuan Co., Ltd. and Hitachi Metals Ltd. held its grand opening ceremony in Qidong City, Jiangsu provinc...Recently, Hitachi Metals San Huan Magnetic Materials (Nantong) Co., Ltd. jointly established by Beijing Zhongke Sanhuan Co., Ltd. and Hitachi Metals Ltd. held its grand opening ceremony in Qidong City, Jiangsu province, which marks its Commencement of business. Hitachi Metals San Huan Magnetic Mateirals (Nantong) Co., Ltd. integrated the advantageous resources of Zhongke Sanhuan and Hitachi Metals.展开更多
基金the National Key R&D Program(2018YFD0500405)the National Nature Science Foundation of China(31972582,U19A2037)+7 种基金the Changsha Natural Science Funds for Distinguished Young Scholar(kq2009020)Special funds for the construction of innovative provinces in Hunan Project(2019NK2193,2019RS3022)Open Fund of Key Laboratory of Agro-ecological Processes in Subtropical Region,Chinese Academy of Sciences(ISA2020203)the‘Strategic Priority Research Program’of the Chinese Academy of Sciences(XDA24030204)the Science and technology projects of Changsha City(kq1801059)Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process(2018TP1031)the General Program of National Natural Science Foundation of China(31872985)the Earmarked Fund for China Agriculture Research System(CARS-35).
文摘The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait,meat quality and lipid metabolism of finishing Huan Jiang mini-pigs.Betaine called trimethylglycine is a methyl derivative of glycine,but few researches were conducted to compare the impact of dietary betaine and glycine on pigs.One hundred and forty-four Huan Jiang mini-pigs(body weight=10.55±0.15 kg;70 d)were randomly divided to 3 treatment groups(basal diet,glycine or betaine).Results indicated that dietary betaine increased the average daily gain(ADG)and final weight(P<0.05).Dietary glycine or betaine markedly reduced average backfat thickness(P<0.05)and heightened lean percentage(P<0.01)compared to the control group.Moreover,in comparison with the control group,betaine significantly improved the redness(a*)and tenderness(shear force)of the longissimus dorsi(LD)muscle(P<0.05),whereas glycine only raised the value of a*of the LD muscle(P<0.05).These results showed that diet supplemented with 0.25%betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs,and the effect of betaine was superior to that of glycine.
基金Under the auspices of National Natural Science Foundation of China(No.41230751,41101547)Scientific Research Foundation of Graduate School of Nanjing University(No.2012CL14)
文摘Hyperspectral data are an important source for monitoring soil salt content on a large scale. However, in previous studies, barriers such as interference due to the presence of vegetation restricted the precision of mapping soil salt content. This study tested a new method for predicting soil salt content with improved precision by using Chinese hyperspectral data, Huan Jing-Hyper Spectral Imager(HJ-HSI), in the coastal area of Rudong County, Eastern China. The vegetation-covered area and coastal bare flat area were distinguished by using the normalized differential vegetation index at the band length of 705 nm(NDVI705). The soil salt content of each area was predicted by various algorithms. A Normal Soil Salt Content Response Index(NSSRI) was constructed from continuum-removed reflectance(CR-reflectance) at wavelengths of 908.95 nm and 687.41 nm to predict the soil salt content in the coastal bare flat area(NDVI705 < 0.2). The soil adjusted salinity index(SAVI) was applied to predict the soil salt content in the vegetation-covered area(NDVI705 ≥ 0.2). The results demonstrate that 1) the new method significantly improves the accuracy of soil salt content mapping(R2 = 0.6396, RMSE = 0.3591), and 2) HJ-HSI data can be used to map soil salt content precisely and are suitable for monitoring soil salt content on a large scale.
基金funding support from the National Natural Science Foundation of China (No. 81302899, 81373551 and 81603512)the Key Science and Research Program of Hunan Department of Science and Technology (No. 2012TF-1005)
文摘Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustion method and Rubrospinal Tract(RST)transection were combined to establish arat model of SCI with pathopattern of QDBS in TCM.Macroscopic representation,including the body weight,food intake and tongue scores,was recorded.Behavioristics was scored with spontaneous vertical exploration test.Hemorrheology was analyzed using hemorheological analyzer and morphology of spinal cord was observed.The feasibility of the model was confirmed on the basis of the reversion caused by administration of Bu Yang Huan Wu Decoction(BYHWD).Results The findings in the QDBS group were consistent with the symptoms of QDBS syndrome,including reduced body weight,food intake,and forelimb utilization rate and increased tongue scores.However,the corresponding findings in the BYHWD group were superior to those in the QDBS group after35d(P<0.05).In comparison with the control group,the QDBS group showed higher whole blood viscosity and hematocrit values(P<0.05)and a lower red blood cell(RBC)deformability index(P<0.05),while the corresponding findings in the BYHWD group were close to those in the control group.An eminence or induration appeared in the injured spinal cord,which was suspected to be vacuoles or a scar.Hematoxylin-eosin staining showed that the R/T ratio in the BYHWD group was higher than that in the QDBS group(P<0.05).Conclusion Swimming exhaustion method combined with RST transection operation can successfully establish a rat model of SCI with pathopattern of QDBS.
基金National Natural Science Foundation of China(Grant No.81373973,81573872,81873228)Science and Technology Planning Project of Guangdong Province(Grant No.2017KZDXM018).
文摘To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and b
文摘With in vitro spin labeling electron spin resonance (ESR) spectroscopy, we have studied the effects of Bu Yang Huan Wu (BYHW) decoction and its effective constituents such as astragaloside IV ferulic acid, chuanxiongzine, rutin, chlorogenic acid, 9,10 dimethoxy pterocarpane 7 O β D glucoside, calycosin, formononetin, calycosin 7 O glucoside, paeoniflorin, paeonal and quercein on the cell membrane fluidity of a rat brain which was modeled after the dual cervical arteries were intercepted and released for realizing an ischemia reperfusion injury which was selected as a brain stroke model. Our results indicated that the cell membrane fluidity in the model group decreased approximately 8% compared with the control group, and after brain cells were incubatied with species, the membrane fluidity could be recovered closely to the control level depending on the BYHW decoction and its different constituents. As the membrane fluidity is a very sensitive biological index which reflectsd the cell status, our method will be useful to study the molecular mechanism of tradition Chinese medicine (TCM) and its combination recipe.
文摘Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.
文摘The author uses the cooperative principle to analyze the conversational implicatures in the Legend of Zhen Huan by examining how the maxims of the cooperative principle are violated.By writing this article, the author hopes to let the audience have a further appreciation of this work and enjoy more about the charms of language.
基金funding support from the Hong Kong Innovation and Technology Commission ITF grant (UIM/289)Grant from National Natural Science Foundation of China (No. 31570855)Contract Research Project of Beijing Tong Ren Tang Chinese Medicine Co. Ltd.
文摘Stroke is the leading cause of human disability with limited effective drugs availability. The disruption of multiple signaling pathways in stroke makes developing new drugs be difficult. Traditional Chinese medicine(TCM) has its unique advantage in targeting multiple signaling pathways with multiple components. Many TCM formulas have the potential for neuroprotection and neurogenesis, but well-designed clinical trials are insufficient and underlying mechanisms unclear. Herein, we introduce the commonly used TCM formulas for stroke treatment and selectively introduce two classic formulas, An Gong Niu Huang(AGNH) pill and Bu Yang Huan Wu Decoction(BYHWD) for neuroprotection and neurogenesis, respectively. Current scientific evidence and clinical trials indicate AGNH pill could be an effective neuroprotective formula as adjunct therapy with relative safety. On the other hand, as a representative TCM formula for post stroke disability, BYHWD could improve the neurological outcome with its neuroprotective and neurogenic effects. The underlying mechanisms could be attributed to the modulation of multiple molecular targets with its multiple components. In conclusion, TCM formulas could be potential adjunct therapies for stroke treatment. The clinical efficacies and molecular mechanisms of promoting neuroprotection and neurogenesis remain to be further studies.
文摘Recently, Hitachi Metals San Huan Magnetic Materials (Nantong) Co., Ltd. jointly established by Beijing Zhongke Sanhuan Co., Ltd. and Hitachi Metals Ltd. held its grand opening ceremony in Qidong City, Jiangsu province, which marks its Commencement of business. Hitachi Metals San Huan Magnetic Mateirals (Nantong) Co., Ltd. integrated the advantageous resources of Zhongke Sanhuan and Hitachi Metals.
