目的探讨肝癌衍生生长因子相关蛋白2（hepatoma-derived growth factor related protein-2,HRP-2）和肝癌衍生生长因子相关蛋白3（HRP-3）在脑胶质瘤中的表达及其与预后的关系。方法选取2005年3月~2012年8月间我院诊治的56例脑胶质瘤患...目的探讨肝癌衍生生长因子相关蛋白2（hepatoma-derived growth factor related protein-2,HRP-2）和肝癌衍生生长因子相关蛋白3（HRP-3）在脑胶质瘤中的表达及其与预后的关系。方法选取2005年3月~2012年8月间我院诊治的56例脑胶质瘤患者为研究对象,以同期56例行颅脑损伤内减压手术患者为对照组,用实时定量聚合酶链反应（RT-QPCR）法测定两组患者HRP-2和HRP-3的表达水平,并分析其与生存时间的相关性。结果胶质瘤组患者的HRP-2和HRP-3表达水平均显著高于对照组,差异具有统计学意义（P〈0.01）;HRP-2高表达组的生存时间与HRP-2低表达组比较,差异无统计学意义（χ^2=0.455,P=0.500）;HRP-3高表达组的生存时间则显著低于HRP-3低表达组（χ^2=5.872,P=0.015）。结论 HRP-2和HRP-3在胶质瘤细胞中均呈现异常高表达,且HRP-3的过表达与患者生存时间缩短紧密相关。展开更多
Background： HRP2-based Rapid Diagnostic Tests （RDTs） for malaria ensure a rational use of artemisinin-based combination therapy （ACT）. The HRP2 antigen can go through the cerebrospinal fluid （CSF）. Purpose： To...Background： HRP2-based Rapid Diagnostic Tests （RDTs） for malaria ensure a rational use of artemisinin-based combination therapy （ACT）. The HRP2 antigen can go through the cerebrospinal fluid （CSF）. Purpose： To assess the HRP2-based RDT in malaria and detect the HRP2 in CSF. Methods： From November 2006 to May 2007, all patients under 15 years showing clinical symptoms of malaria were included. RDT was performed on the CSF and peripheral blood. Results： Out of the 951 patients included, 131 （13.7%） were confirmed through RDT and 130 （13.6%） through thick blood smear. Sensitivity and specificity stood at 96.96% and 99.71% respectively, for uncomplicated cases and at 100% and 99.13% for severe cases. Tests to detect HRP2 in the CSF of 52 cases were negative. Conclusion： Tests to detect HRP2 in the CSF could make it possible to diagnose severe malaria cases when peripheral parasitemia would be below the detection threshold.展开更多
Fifteen non-aminoazo, dyes were oxidized when catalyzed by horseradish peroxidase (HRP) in the presence of H2O2. Aromaticdiazonium ions, the ultimate carcinogen, were detected in the reaction with all of azo compounds...Fifteen non-aminoazo, dyes were oxidized when catalyzed by horseradish peroxidase (HRP) in the presence of H2O2. Aromaticdiazonium ions, the ultimate carcinogen, were detected in the reaction with all of azo compounds as the substrates for HRP. The rates of oxidative cleavage fairly depended on pH. Relative enzymatic activities were compared among 15 non-aminoazo, dyes, and the structural requirements of the substrates for HRP were discussed in detail.展开更多
文摘Background： HRP2-based Rapid Diagnostic Tests （RDTs） for malaria ensure a rational use of artemisinin-based combination therapy （ACT）. The HRP2 antigen can go through the cerebrospinal fluid （CSF）. Purpose： To assess the HRP2-based RDT in malaria and detect the HRP2 in CSF. Methods： From November 2006 to May 2007, all patients under 15 years showing clinical symptoms of malaria were included. RDT was performed on the CSF and peripheral blood. Results： Out of the 951 patients included, 131 （13.7%） were confirmed through RDT and 130 （13.6%） through thick blood smear. Sensitivity and specificity stood at 96.96% and 99.71% respectively, for uncomplicated cases and at 100% and 99.13% for severe cases. Tests to detect HRP2 in the CSF of 52 cases were negative. Conclusion： Tests to detect HRP2 in the CSF could make it possible to diagnose severe malaria cases when peripheral parasitemia would be below the detection threshold.
基金Project supported by the National Natural Science Foundation of China.
文摘Fifteen non-aminoazo, dyes were oxidized when catalyzed by horseradish peroxidase (HRP) in the presence of H2O2. Aromaticdiazonium ions, the ultimate carcinogen, were detected in the reaction with all of azo compounds as the substrates for HRP. The rates of oxidative cleavage fairly depended on pH. Relative enzymatic activities were compared among 15 non-aminoazo, dyes, and the structural requirements of the substrates for HRP were discussed in detail.
