Background Type 1 diabetes （TID） is a multifactorial disease. This article aims to evaluate the relationship between allele polymorphism of HLA-DQ, DR and TID in the Chinese population. Methods The odds ratios （ORs...Background Type 1 diabetes （TID） is a multifactorial disease. This article aims to evaluate the relationship between allele polymorphism of HLA-DQ, DR and TID in the Chinese population. Methods The odds ratios （ORs） of HLA-DQ, DR allele distributions in patients with T1D were analyzed against healthy controls. All the relevant studies in Pubmed and CNKI were identified, and poor qualified studies were excluded. The meta-analysis software REVMAN 4.2 was applied for investigating heterogeneity among individual studies and for summarizing all the studies. The publication bias were also evaluated. Results DQA1＊0301, DQA1＊0501, DQB1＊0201, DQB1＊0302 were the susceptible alleles （all P 〈0.05） in the Chinese population, their merger ORs 2.40, 3.15, 3.66, and 2.67 respectively. DQA1＊0103, DQA1＊0201, DQA1＊0401, DQB1＊0301, DQB1＊0402, DQB1 ＊0501, DQB1＊0503, DQB1＊0601 and DQB1＊0602 were the protective alleles （P 〈0.05）, their merger ORs were 0.11, 0.45, 0.30, 0.38, 0.23, 0.37, 0.25, 0.48, and 0.30 respectively. In serum level, DR3, DR4, DR9 alleles were the susceptible alleles （all P 〈0.05） and their merger ORs were 5.58, 1.53, 1.66, 29.78, and 6.65 respectively. HLA-DR2, DR5, and DR7 alleles were the protective alleles （all P 〈0.05） and their merger ORs were 0.39, 0.51, and 0.50. In genetic type level, DRB1＊04, DRB1＊0301, DRB1＊0901 were the susceptible alleles （all P 〈0.05） and their merger ORs were 2.19, 6.43, 1.31, 3.83, and 8.08. DRBI＊07, DRBI＊08, DRB1＊12, DRB1＊13, DRB1＊14, DRB1＊16, DRBI＊0406 alleles were the protective alleles （all P 〈0.05） and their merger ORswere 0.44, 0.27, 0.45, 0.13, 0.19, 0.40, and 0.27 respectively. Conclusions In the Chinese population, some HLA-DQ, DR alleles are relevant to T1D which are not totally the same as non-Chinese populations.展开更多
Background The frequencies of regulatory T cells （Tregs） increased over the HIV infection but its counts actually decreased. We proposed that the decrease of Treg counts may cause the reduction of inhibitory effect ...Background The frequencies of regulatory T cells （Tregs） increased over the HIV infection but its counts actually decreased. We proposed that the decrease of Treg counts may cause the reduction of inhibitory effect and thereby account for the over-activation of Tregs during HIV infection. However, it remains unknown whether Tregs are also over-activated and thereafter the activation induced death may lead to the decrease of Tregs. Methods Tregs were defined as CD4＋CD25＋CD127lo/-T cells. Eighty-one HIV-1 infected patients were enrolled in our study, and twenty-two HIV-1 seronegative donors were recruited as the control. The levels of HLA-DR on Tregs were determined by FACSAria flow cytometer. Results Compared to HIV-1 seronegative donors, the levels of HLA-DR on CD4＋CD25＋CD127lo/- Tregs were significantly increased in HIV-1 infected patients, and its increase was positively associated with viral loads （r=0.3163, P=-0.004） and negatively with CD4 T-cell counts （r=-0.4153, P 〈0.0001）. In addition, significant associations between HLA-DR expression on CD4＋CD25＋CD127lo/- Tregs and the percentages of HLA-DR, CD38, Ki67 expressing CD4＋ and CD8＋ T cells were also identified. Conclusion HLA-DR on Tregs is a good marker for viral replication and disease progression. The over-activation of Tregs might result in the decrease of Tregs.展开更多
文摘Background Type 1 diabetes （TID） is a multifactorial disease. This article aims to evaluate the relationship between allele polymorphism of HLA-DQ, DR and TID in the Chinese population. Methods The odds ratios （ORs） of HLA-DQ, DR allele distributions in patients with T1D were analyzed against healthy controls. All the relevant studies in Pubmed and CNKI were identified, and poor qualified studies were excluded. The meta-analysis software REVMAN 4.2 was applied for investigating heterogeneity among individual studies and for summarizing all the studies. The publication bias were also evaluated. Results DQA1＊0301, DQA1＊0501, DQB1＊0201, DQB1＊0302 were the susceptible alleles （all P 〈0.05） in the Chinese population, their merger ORs 2.40, 3.15, 3.66, and 2.67 respectively. DQA1＊0103, DQA1＊0201, DQA1＊0401, DQB1＊0301, DQB1＊0402, DQB1 ＊0501, DQB1＊0503, DQB1＊0601 and DQB1＊0602 were the protective alleles （P 〈0.05）, their merger ORs were 0.11, 0.45, 0.30, 0.38, 0.23, 0.37, 0.25, 0.48, and 0.30 respectively. In serum level, DR3, DR4, DR9 alleles were the susceptible alleles （all P 〈0.05） and their merger ORs were 5.58, 1.53, 1.66, 29.78, and 6.65 respectively. HLA-DR2, DR5, and DR7 alleles were the protective alleles （all P 〈0.05） and their merger ORs were 0.39, 0.51, and 0.50. In genetic type level, DRB1＊04, DRB1＊0301, DRB1＊0901 were the susceptible alleles （all P 〈0.05） and their merger ORs were 2.19, 6.43, 1.31, 3.83, and 8.08. DRBI＊07, DRBI＊08, DRB1＊12, DRB1＊13, DRB1＊14, DRB1＊16, DRBI＊0406 alleles were the protective alleles （all P 〈0.05） and their merger ORswere 0.44, 0.27, 0.45, 0.13, 0.19, 0.40, and 0.27 respectively. Conclusions In the Chinese population, some HLA-DQ, DR alleles are relevant to T1D which are not totally the same as non-Chinese populations.
文摘Background The frequencies of regulatory T cells （Tregs） increased over the HIV infection but its counts actually decreased. We proposed that the decrease of Treg counts may cause the reduction of inhibitory effect and thereby account for the over-activation of Tregs during HIV infection. However, it remains unknown whether Tregs are also over-activated and thereafter the activation induced death may lead to the decrease of Tregs. Methods Tregs were defined as CD4＋CD25＋CD127lo/-T cells. Eighty-one HIV-1 infected patients were enrolled in our study, and twenty-two HIV-1 seronegative donors were recruited as the control. The levels of HLA-DR on Tregs were determined by FACSAria flow cytometer. Results Compared to HIV-1 seronegative donors, the levels of HLA-DR on CD4＋CD25＋CD127lo/- Tregs were significantly increased in HIV-1 infected patients, and its increase was positively associated with viral loads （r=0.3163, P=-0.004） and negatively with CD4 T-cell counts （r=-0.4153, P 〈0.0001）. In addition, significant associations between HLA-DR expression on CD4＋CD25＋CD127lo/- Tregs and the percentages of HLA-DR, CD38, Ki67 expressing CD4＋ and CD8＋ T cells were also identified. Conclusion HLA-DR on Tregs is a good marker for viral replication and disease progression. The over-activation of Tregs might result in the decrease of Tregs.
