Viral load measurements are an essential tool for the long-term clinical care of human immunodeficiency virus （HIV）-positive individuals. The gold standards in viral load instrumentation, however, are still too limi...Viral load measurements are an essential tool for the long-term clinical care of human immunodeficiency virus （HIV）-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification （RT-LAMP） on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a - 60 nL RT- LAMP droplet, corresponding to a whole blood concentration of 670 viruses per μL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a finger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation.展开更多
目的系统描述和分析HIV/AIDS患者家属照顾者照护负担的影响因素。方法计算机辅助手工检索Pubmed、CINAHL、Web of Science、荷兰生物医学文摘数据库、中国生物医学文献数据库、中国知网、维普、万方等数据库中有关HIV/AIDS患者的家属照...目的系统描述和分析HIV/AIDS患者家属照顾者照护负担的影响因素。方法计算机辅助手工检索Pubmed、CINAHL、Web of Science、荷兰生物医学文摘数据库、中国生物医学文献数据库、中国知网、维普、万方等数据库中有关HIV/AIDS患者的家属照顾者照护负担影响因素的定量研究。根据文献纳入和排除标准对检索到的文献进行独立筛选、质量评价和资料提取,采用定性分析的方法归纳总结研究结果。结果共纳入12项横断面研究,影响HIV/AIDS患者家属照顾者照护负担的因素包括患者因素、照顾者因素和社会因素。结论患者的照护需求,照顾者的身体状况、经济状况、应对方式、心理状况及HIV病耻感对照护负担的影响得到了一致的结论,卫生保健人员应该针对可干预的影响因素为照顾者制定个体化的减轻照护负担的干预措施。展开更多
目的了解江苏省社会组织参与国家艾滋病防治基金(China AIDS fund for non-governmental organizations, CAFNGO)项目整体情况,促进其更积极主动参与艾滋病防治工作。方法读取CAFNGO项目管理信息系统2016—2020年社会组织每季度填报的数...目的了解江苏省社会组织参与国家艾滋病防治基金(China AIDS fund for non-governmental organizations, CAFNGO)项目整体情况,促进其更积极主动参与艾滋病防治工作。方法读取CAFNGO项目管理信息系统2016—2020年社会组织每季度填报的数据,对资料进行整理和分析。结果 2016—2020年,江苏省有11个设区市的社会组织参与139个项目,其中女性商业性工作者(female sex workers, FSW)干预类项目25个(17.98%),男男同性性行为人群(men who have sex with men, MSM)干预项目59个(42.45%),吸毒者干预项目4个(2.88%),HIV感染者和患者(people living with HIV,PLWH)随访管理和关怀救助类项目51个(36.69%)。MSM HIV抗体阳性率由2016年4.52%下降至2020年3.13%,累计HIV抗体阳性1 748例,阳性率3.81%。2017—2020年,成功转介抗病毒治疗人数占HIV抗体阳性数的83.82%(1 145/1 366)。对8 964例PLWH开展了随访管理和关怀救助,8 868例(98.93%)符合抗病毒治疗条件,其中接受抗病毒治疗8 732例(98.47%),完成病毒载量检测6 553例,病例病毒载量检测<1 000拷贝/ml 6 376例(97.30%)。结论 CAFNGO支持江苏省社会组织工作成为艾滋病防治领域的重要内容,应发挥社会组织工作的自主性与积极性,进一步预防艾滋病传播。展开更多
In the last decade, the long-term survival among people living with HIV (PLHIV) has significantly improved. This is accompanied by an increased burden of non-communicable diseases such as hypertension due to the combi...In the last decade, the long-term survival among people living with HIV (PLHIV) has significantly improved. This is accompanied by an increased burden of non-communicable diseases such as hypertension due to the combined effect of the aging population and the metabolic effect of the Human Immuno-deficiency Virus (HIV) virion and antiretroviral therapy. This study aims to assess the prevalence and factors associated with hypertension among people living with HIV in three large health facilities in Nasarawa State, Nigeria. A descriptive cross-sectional study employed a multistage sampling technique to select 309 adults with HIV, 18 years and above, receiving HIV care in three large health facilities in Nasarawa State. The outcome variable was the participants’ self-reported history of hypertension, confirmed through a positive history of hypertension treatment. Exposure variables included the participant’s socio-demographic characteristics, lifestyle factors, and HIV care and treatment history. Data were presented using frequency tables. Factors associated with hypertension were assessed using binary logistic regression at a 0.05 level of statistical significance. A total of 309 adults living with HIV were sampled. A larger percentage of the participants were married 228 (73.8%), female, 191 (61.8%), within the age group 41 - 50 years, 141 (45.6%). Most of the participants had no family history of hypertension, 188 (60.8%). The prevalence of self-reported hypertension was 11.0% (34/309). Factors associated with hypertension at the bivariate level were age group 21 - 30 years, 41 - 50 years, being widow/widower, divorced, retired from employment or with family history of hypertension. Only participants age group 31 - 40 years [Adjusted Odd Ratio (AOR): 0.18, 95%CI: 0.04 - 0.91, p = 0.04] and family history of hypertension [(AOR): 83.44, 95%CI: 15.75 - 442.11, p < 0.01] were found to predict hypertension among the study participants after adjusting for confounders. In conclusion, Hypertension remains a public hea展开更多
Ozone is a green broad-spectrum bactericidal disinfectant, and a trace amount of ozone in the atmosphere makes many viruses and bacteria lose their biochemical activity and infectivity. Nature produces trace amounts o...Ozone is a green broad-spectrum bactericidal disinfectant, and a trace amount of ozone in the atmosphere makes many viruses and bacteria lose their biochemical activity and infectivity. Nature produces trace amounts of ozone in the air through lightning to purify the ecological environment. The product of ozone decomposition is oxygen, without secondary pollution. Ozone sterilizer is widely used in the epidemic prevention and control of intensive breeding farms and achieved remarkable results. If the concentration and action time of ozone can be accurately controlled, then ozone can quickly eliminate pathogens, without harming the normal cells in the human body. How to use medical ozone for epidemic prevention, treatment and health care is a subject worthy of serious study, which should arouse the attention of the experts in the field.展开更多
基金supported by funding from the National Institutes of Health (NIH) Exploratory/Developmental Grant (R21) (AI106024)supported by a Ruth L.Kirschstein National Research Service Award for Individual Pred octoral MD/PhD and Other Dual Doctoral Degree Fellows (F30) (AI109825)
文摘Viral load measurements are an essential tool for the long-term clinical care of human immunodeficiency virus （HIV）-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification （RT-LAMP） on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a - 60 nL RT- LAMP droplet, corresponding to a whole blood concentration of 670 viruses per μL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a finger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation.
文摘目的系统描述和分析HIV/AIDS患者家属照顾者照护负担的影响因素。方法计算机辅助手工检索Pubmed、CINAHL、Web of Science、荷兰生物医学文摘数据库、中国生物医学文献数据库、中国知网、维普、万方等数据库中有关HIV/AIDS患者的家属照顾者照护负担影响因素的定量研究。根据文献纳入和排除标准对检索到的文献进行独立筛选、质量评价和资料提取,采用定性分析的方法归纳总结研究结果。结果共纳入12项横断面研究,影响HIV/AIDS患者家属照顾者照护负担的因素包括患者因素、照顾者因素和社会因素。结论患者的照护需求,照顾者的身体状况、经济状况、应对方式、心理状况及HIV病耻感对照护负担的影响得到了一致的结论,卫生保健人员应该针对可干预的影响因素为照顾者制定个体化的减轻照护负担的干预措施。
文摘目的了解江苏省社会组织参与国家艾滋病防治基金(China AIDS fund for non-governmental organizations, CAFNGO)项目整体情况,促进其更积极主动参与艾滋病防治工作。方法读取CAFNGO项目管理信息系统2016—2020年社会组织每季度填报的数据,对资料进行整理和分析。结果 2016—2020年,江苏省有11个设区市的社会组织参与139个项目,其中女性商业性工作者(female sex workers, FSW)干预类项目25个(17.98%),男男同性性行为人群(men who have sex with men, MSM)干预项目59个(42.45%),吸毒者干预项目4个(2.88%),HIV感染者和患者(people living with HIV,PLWH)随访管理和关怀救助类项目51个(36.69%)。MSM HIV抗体阳性率由2016年4.52%下降至2020年3.13%,累计HIV抗体阳性1 748例,阳性率3.81%。2017—2020年,成功转介抗病毒治疗人数占HIV抗体阳性数的83.82%(1 145/1 366)。对8 964例PLWH开展了随访管理和关怀救助,8 868例(98.93%)符合抗病毒治疗条件,其中接受抗病毒治疗8 732例(98.47%),完成病毒载量检测6 553例,病例病毒载量检测<1 000拷贝/ml 6 376例(97.30%)。结论 CAFNGO支持江苏省社会组织工作成为艾滋病防治领域的重要内容,应发挥社会组织工作的自主性与积极性,进一步预防艾滋病传播。
文摘In the last decade, the long-term survival among people living with HIV (PLHIV) has significantly improved. This is accompanied by an increased burden of non-communicable diseases such as hypertension due to the combined effect of the aging population and the metabolic effect of the Human Immuno-deficiency Virus (HIV) virion and antiretroviral therapy. This study aims to assess the prevalence and factors associated with hypertension among people living with HIV in three large health facilities in Nasarawa State, Nigeria. A descriptive cross-sectional study employed a multistage sampling technique to select 309 adults with HIV, 18 years and above, receiving HIV care in three large health facilities in Nasarawa State. The outcome variable was the participants’ self-reported history of hypertension, confirmed through a positive history of hypertension treatment. Exposure variables included the participant’s socio-demographic characteristics, lifestyle factors, and HIV care and treatment history. Data were presented using frequency tables. Factors associated with hypertension were assessed using binary logistic regression at a 0.05 level of statistical significance. A total of 309 adults living with HIV were sampled. A larger percentage of the participants were married 228 (73.8%), female, 191 (61.8%), within the age group 41 - 50 years, 141 (45.6%). Most of the participants had no family history of hypertension, 188 (60.8%). The prevalence of self-reported hypertension was 11.0% (34/309). Factors associated with hypertension at the bivariate level were age group 21 - 30 years, 41 - 50 years, being widow/widower, divorced, retired from employment or with family history of hypertension. Only participants age group 31 - 40 years [Adjusted Odd Ratio (AOR): 0.18, 95%CI: 0.04 - 0.91, p = 0.04] and family history of hypertension [(AOR): 83.44, 95%CI: 15.75 - 442.11, p < 0.01] were found to predict hypertension among the study participants after adjusting for confounders. In conclusion, Hypertension remains a public hea
文摘Ozone is a green broad-spectrum bactericidal disinfectant, and a trace amount of ozone in the atmosphere makes many viruses and bacteria lose their biochemical activity and infectivity. Nature produces trace amounts of ozone in the air through lightning to purify the ecological environment. The product of ozone decomposition is oxygen, without secondary pollution. Ozone sterilizer is widely used in the epidemic prevention and control of intensive breeding farms and achieved remarkable results. If the concentration and action time of ozone can be accurately controlled, then ozone can quickly eliminate pathogens, without harming the normal cells in the human body. How to use medical ozone for epidemic prevention, treatment and health care is a subject worthy of serious study, which should arouse the attention of the experts in the field.
