A rapid and sensitive LC-MS/MS method was established to analyze melamine and cyanuric acid in pet food and its ingredients.HILIC mechanism was utilized to separate small highly polar compounds such as melamine and cy...A rapid and sensitive LC-MS/MS method was established to analyze melamine and cyanuric acid in pet food and its ingredients.HILIC mechanism was utilized to separate small highly polar compounds such as melamine and cyanuric acid.In comparison with US FDA recognized GCMS and LC methods,it turned out that the current technique is superior with easy sample preparation,and the sensitivity and selectivity was found satisfactory.The LOD was 50 ng·L-1 for melamine and 1 μg·L-1 for cyanuric acid,respectively.展开更多
建立了动物源性食品中15种抗病毒类药物残留的通过式净化-亲水作用色谱-串联质谱分析方法。样品经1%酸化乙腈溶液超声提取,冷冻离心后,经PRi ME HLB小柱净化,直接进样分析。以资生堂PC HILIC色谱柱进行分离,以10 mmol/L乙酸铵(含0.2%甲...建立了动物源性食品中15种抗病毒类药物残留的通过式净化-亲水作用色谱-串联质谱分析方法。样品经1%酸化乙腈溶液超声提取,冷冻离心后,经PRi ME HLB小柱净化,直接进样分析。以资生堂PC HILIC色谱柱进行分离,以10 mmol/L乙酸铵(含0.2%甲酸)和乙腈为流动相进行梯度洗脱,电喷雾正离子(ESI+)模式电离、多反应监测(MRM)模式进行检测,基质匹配标准工作曲线定量。结果表明,15种抗病毒药物在0.2~80μg/kg范围内呈良好线性关系,相关系数(r^(2))均大于0.999,15种抗病毒药物的检出限为0.1~1.0μg/kg之间,0.3,5.0,20μg/kg 3水平的加标回收率在65.9%~115%之间,RSDs为1.7%~9.2%。展开更多
文摘A rapid and sensitive LC-MS/MS method was established to analyze melamine and cyanuric acid in pet food and its ingredients.HILIC mechanism was utilized to separate small highly polar compounds such as melamine and cyanuric acid.In comparison with US FDA recognized GCMS and LC methods,it turned out that the current technique is superior with easy sample preparation,and the sensitivity and selectivity was found satisfactory.The LOD was 50 ng·L-1 for melamine and 1 μg·L-1 for cyanuric acid,respectively.
