Publish/subscribe(pub/sub) paradigm is the main communication model for Information-Centric Network(ICN) proposals.A key issue for pub/sub system is how to route the content objects to the correct subscribers,and ICN ...Publish/subscribe(pub/sub) paradigm is the main communication model for Information-Centric Network(ICN) proposals.A key issue for pub/sub system is how to route the content objects to the correct subscribers,and ICN is no exception.ICN network would be divided into core domain and many edge domains as today's internet does.HHR(Hierarchy Hybrid Routing scheme) is presented for ICN:A Chord-like routing scheme is used in core domain,while edge domains routing structure can be classified into three categories,Local Routing(LR),Delivery of Local Publication to Core domain(DLPC),and Remote Publication Routing into edge domain(RPR).LR can be decided by each edge domain,which determined by many factors,such as locality characteristic for pub/sub information and local policies.A hierarchical routing algorithm is proposed to solve DLPC and RPR simultaneously.Simulation results demonstrate that HHR can be fast deployed,and can be applied in large scale network or dynamic subscription environment.展开更多
Objective The radiation sensitive gene rad 21 of Schizosaccharomyces pombe is involved in the repair of double-stranded breaks in DNA and is essential for mitotic growth. The hHR21 sp g...Objective The radiation sensitive gene rad 21 of Schizosaccharomyces pombe is involved in the repair of double-stranded breaks in DNA and is essential for mitotic growth. The hHR21 sp gene is its human homologue. In an attempt to investigate the role of hHR21 sp in DNA repair, we studied the effects of UV and γ-ray irradiation on hHR21 sp gene expression in normal human peripheral blood cells, and non-iradiated peripheral and bone marrow cells from Fanconi anemia (FA) patients who have shown DNA repair deficiency.Methods Total steady state RNA was extracted from peripheral blood cells and bone marrow. RNA transcripts were quantified after RT-PCR and Southern blot, phosphoimmage and autoradiogram analysis. The results were compared with control groups. Results hHR21 sp expression was significantly increased from 3?h to 9?h after UV irradiation in peripheral blood cells from normal subjects at doses of 40-80?j/m 2 (P<0.05). hHR21 sp was also up-regulated by γ-ray irradiation at 6?h to 9?h at dose of 1 to 5?Gy (P<0.01), which was more significant than the UV irradiation. In the non-irradiated FA patient group, hHR21 sp expression was decreased in bone marrow hematopoietic cells (P<0.05). After activation by PHA and IL-2, there was still a significant depression in expression by the FA patients peripheral blood cells compared with control groups (P<0.05). Conclusion hHR21 sp was up-regulated at doses and times irradiated at the range tested in normal peripheral blood cells, and is more affected by γ-ray irradiation than UV irradiation. FA patient bone marrow hematopoietic cells and peripheral blood mononuclear cells showed down-regulation of hHR21 sp expression. The results imply that defects in DNA repair via hHR21 sp expression may play an important role in the pathogenesis of FA syndrome.展开更多
Basin-scale projections of river runoff at different warming levels provide useful information for climate change adaptation. In this study, we investigated changes in the projected climate and simulated runoff under ...Basin-scale projections of river runoff at different warming levels provide useful information for climate change adaptation. In this study, we investigated changes in the projected climate and simulated runoff under 1.5℃ and 2.0℃ global warming of three inland rivers in the Hexi Corridor: the Shiyang River(SYR), the Heihe River(HHR),and the Shule River(SLR). The change in climate was projected based on five global climate models(GCMs) under three representative concentration pathways(RCPs), and the change in runoff was simulated based on the Soil and Water Assessment Tool(SWAT) hydrological model. Furthermore, the uncertainties in projected climate change and simulated runoff constrained by the GCMs and RCPs were quantified. The results indicate that, compared with the baseline period(1976–2005), there is a 1.42–1.54℃ increase in annual air temperature and 4%–12% increase in annual mean precipitation in the three river basins under 1.5℃ global warming, while there is a 2.09–2.36℃ increase in annual air temperature and 5%–11% increase in annual mean precipitation under 2.0℃ global warming. The simulated annual runoff of the SYR decreases by 4% under 1.5℃ global warming, that of the HHR decreases by 3% and 4%, while that of the SLR increases considerably by 10% and 11% under 1.5℃ and 2.0℃ global warming, respectively. The additional 0.5℃ global warming results in an annual air temperature increase of 0.67–0.82℃, a change of -1% to 1% in annual mean precipitation, and a change of -1% to 5% in simulated runoff. The simulated annual runoff has greater uncertainty. The simulations indicate substantial and consistent warming in autumn and winter in the three basins, relatively drier summer and autumn in the SYR and HHR basins, and a relatively drier autumn in the SLR basin. The simulated monthly runoff shows more complex changes with large uncertainties constrained mainly by the GCMs.展开更多
In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera, Helicoverpa hormone receptor 3 （HHR3）, which is involved in the ecdysteroid regulatory pathway, was used to geneti...In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera, Helicoverpa hormone receptor 3 （HHR3）, which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps： preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus.HHR3 was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth.展开更多
基金supported by 973 Program(2013CB329103)NSFC Fund (61271165,61301153)Program for Changjiang Scholars and Innovative Research Team (PCSIRT) in University and the 111 Project B14039
文摘Publish/subscribe(pub/sub) paradigm is the main communication model for Information-Centric Network(ICN) proposals.A key issue for pub/sub system is how to route the content objects to the correct subscribers,and ICN is no exception.ICN network would be divided into core domain and many edge domains as today's internet does.HHR(Hierarchy Hybrid Routing scheme) is presented for ICN:A Chord-like routing scheme is used in core domain,while edge domains routing structure can be classified into three categories,Local Routing(LR),Delivery of Local Publication to Core domain(DLPC),and Remote Publication Routing into edge domain(RPR).LR can be decided by each edge domain,which determined by many factors,such as locality characteristic for pub/sub information and local policies.A hierarchical routing algorithm is proposed to solve DLPC and RPR simultaneously.Simulation results demonstrate that HHR can be fast deployed,and can be applied in large scale network or dynamic subscription environment.
文摘Objective The radiation sensitive gene rad 21 of Schizosaccharomyces pombe is involved in the repair of double-stranded breaks in DNA and is essential for mitotic growth. The hHR21 sp gene is its human homologue. In an attempt to investigate the role of hHR21 sp in DNA repair, we studied the effects of UV and γ-ray irradiation on hHR21 sp gene expression in normal human peripheral blood cells, and non-iradiated peripheral and bone marrow cells from Fanconi anemia (FA) patients who have shown DNA repair deficiency.Methods Total steady state RNA was extracted from peripheral blood cells and bone marrow. RNA transcripts were quantified after RT-PCR and Southern blot, phosphoimmage and autoradiogram analysis. The results were compared with control groups. Results hHR21 sp expression was significantly increased from 3?h to 9?h after UV irradiation in peripheral blood cells from normal subjects at doses of 40-80?j/m 2 (P<0.05). hHR21 sp was also up-regulated by γ-ray irradiation at 6?h to 9?h at dose of 1 to 5?Gy (P<0.01), which was more significant than the UV irradiation. In the non-irradiated FA patient group, hHR21 sp expression was decreased in bone marrow hematopoietic cells (P<0.05). After activation by PHA and IL-2, there was still a significant depression in expression by the FA patients peripheral blood cells compared with control groups (P<0.05). Conclusion hHR21 sp was up-regulated at doses and times irradiated at the range tested in normal peripheral blood cells, and is more affected by γ-ray irradiation than UV irradiation. FA patient bone marrow hematopoietic cells and peripheral blood mononuclear cells showed down-regulation of hHR21 sp expression. The results imply that defects in DNA repair via hHR21 sp expression may play an important role in the pathogenesis of FA syndrome.
基金Supported by the National Key Research and Development Program of China(2018YFA0606302 and SQ2018YFE010367)China Meteorological Administration Climate Change Project(CCSF201924)。
文摘Basin-scale projections of river runoff at different warming levels provide useful information for climate change adaptation. In this study, we investigated changes in the projected climate and simulated runoff under 1.5℃ and 2.0℃ global warming of three inland rivers in the Hexi Corridor: the Shiyang River(SYR), the Heihe River(HHR),and the Shule River(SLR). The change in climate was projected based on five global climate models(GCMs) under three representative concentration pathways(RCPs), and the change in runoff was simulated based on the Soil and Water Assessment Tool(SWAT) hydrological model. Furthermore, the uncertainties in projected climate change and simulated runoff constrained by the GCMs and RCPs were quantified. The results indicate that, compared with the baseline period(1976–2005), there is a 1.42–1.54℃ increase in annual air temperature and 4%–12% increase in annual mean precipitation in the three river basins under 1.5℃ global warming, while there is a 2.09–2.36℃ increase in annual air temperature and 5%–11% increase in annual mean precipitation under 2.0℃ global warming. The simulated annual runoff of the SYR decreases by 4% under 1.5℃ global warming, that of the HHR decreases by 3% and 4%, while that of the SLR increases considerably by 10% and 11% under 1.5℃ and 2.0℃ global warming, respectively. The additional 0.5℃ global warming results in an annual air temperature increase of 0.67–0.82℃, a change of -1% to 1% in annual mean precipitation, and a change of -1% to 5% in simulated runoff. The simulated annual runoff has greater uncertainty. The simulations indicate substantial and consistent warming in autumn and winter in the three basins, relatively drier summer and autumn in the SYR and HHR basins, and a relatively drier autumn in the SLR basin. The simulated monthly runoff shows more complex changes with large uncertainties constrained mainly by the GCMs.
基金Supported by the National Natural Science Foundation of China （30330070）, the High Technology Research and Development Programme of China （2003AA214050） and the Natural Science Foundation of Shandong Province （Z2003D04）. Received on June 13, 2006
文摘In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera, Helicoverpa hormone receptor 3 （HHR3）, which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps： preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus.HHR3 was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth.
