To explore the effects of cadmium (Cd 2+ ) exposure on the antioxidant enzyme activity of the hepatapancreas, freshwater crabs Sinopotamon yangtsekiense were exposed to Cd 2+ (7.25 mg/L, 14.5 mg/L, 29 mg/L, 58 mg/L, 1...To explore the effects of cadmium (Cd 2+ ) exposure on the antioxidant enzyme activity of the hepatapancreas, freshwater crabs Sinopotamon yangtsekiense were exposed to Cd 2+ (7.25 mg/L, 14.5 mg/L, 29 mg/L, 58 mg/L, 116 mg/L) for 24, 48, 72 and 96 hours. The activities of antioxidant enzymes were investigated including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), GSSG-reductase (GR) and glutathione S-transferase (GST), and the GPX/SOD, GR/GPX, CAT/SOD ratios, as indicators of antioxidant properties. Results showed that the activities of SOD, CAT and GPX increased initially and decreased subsequently, but GR and GST activities remained almost unchanged. The changes in antioxidant ratios of GPX/SOD, GR/GPX and CAT/SOD indicated that, following the increase of concentration of Cd 2+ and duration of treatment, significant cellular injuries were caused in the hepatapancreas of S.yangtsekiense due to the oxidative stress. We conclude that the changes of the indicators (SOD, CAT, GPX as well as GPX/SOD, GR/GPX, CAT/SOD ratios) may exquisitely reflect the oxidative stress in an aquatic animal induced by Cd 2+ and the toxicity of it.展开更多
The metallothionein（MT） concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time（P<0.05）,whereas that in th...The metallothionein（MT） concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time（P<0.05）,whereas that in the control groups had no marked changes（P>0.05）.Exposed to three heavy metal ions,the MT concentration in hepatopancreas changed very significantly in 6 h（P<0.01）. The MT concentration inducted by Cu2+(0.1,0.2,0.5,1 mg·L-1), Zn2+(1,2, 5 mg·L-1)and Cd2+(0.05,0.1 mg·L-1)increased in 96 h with time and changed stably after 48 h.In addition,the MT concentration inducted by Zn2+(10 mg·L-1) and Cd2+(0.25,0.5 mg·L-1)was up to max.at 48 h,began to decrease with time passing by,and still higher than that in the control groups.Meanwhile the inductive ratio of three heavy metal ions on metallothionein concentration were Cd2+>Cu2+>Zn2+ in experimental time.展开更多
文摘To explore the effects of cadmium (Cd 2+ ) exposure on the antioxidant enzyme activity of the hepatapancreas, freshwater crabs Sinopotamon yangtsekiense were exposed to Cd 2+ (7.25 mg/L, 14.5 mg/L, 29 mg/L, 58 mg/L, 116 mg/L) for 24, 48, 72 and 96 hours. The activities of antioxidant enzymes were investigated including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), GSSG-reductase (GR) and glutathione S-transferase (GST), and the GPX/SOD, GR/GPX, CAT/SOD ratios, as indicators of antioxidant properties. Results showed that the activities of SOD, CAT and GPX increased initially and decreased subsequently, but GR and GST activities remained almost unchanged. The changes in antioxidant ratios of GPX/SOD, GR/GPX and CAT/SOD indicated that, following the increase of concentration of Cd 2+ and duration of treatment, significant cellular injuries were caused in the hepatapancreas of S.yangtsekiense due to the oxidative stress. We conclude that the changes of the indicators (SOD, CAT, GPX as well as GPX/SOD, GR/GPX, CAT/SOD ratios) may exquisitely reflect the oxidative stress in an aquatic animal induced by Cd 2+ and the toxicity of it.
文摘The metallothionein（MT） concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time（P<0.05）,whereas that in the control groups had no marked changes（P>0.05）.Exposed to three heavy metal ions,the MT concentration in hepatopancreas changed very significantly in 6 h（P<0.01）. The MT concentration inducted by Cu2+(0.1,0.2,0.5,1 mg·L-1), Zn2+(1,2, 5 mg·L-1)and Cd2+(0.05,0.1 mg·L-1)increased in 96 h with time and changed stably after 48 h.In addition,the MT concentration inducted by Zn2+(10 mg·L-1) and Cd2+(0.25,0.5 mg·L-1)was up to max.at 48 h,began to decrease with time passing by,and still higher than that in the control groups.Meanwhile the inductive ratio of three heavy metal ions on metallothionein concentration were Cd2+>Cu2+>Zn2+ in experimental time.
