Background We distinguished graft-versus-host disease (GVHD) from graft-versus-leukemia (GVL) effects and to investigate the distribution of T-cell receptor (TCR) Vβ gene repertoire in individuals with leukemia befor...Background We distinguished graft-versus-host disease (GVHD) from graft-versus-leukemia (GVL) effects and to investigate the distribution of T-cell receptor (TCR) Vβ gene repertoire in individuals with leukemia before and after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods Peripheral blood mononuclear cells (PBMC) were obtained from 10 normal individuals, 8 donors and 11 patients with leukemia before and after transplantation. Polymerase chain reaction (PCR) amplification of complementarity-determining region 3 (CDR3) of 24 TCR Vβ genes was used to examine serial samples of PBMC. The PCR products were further analyzed by genescan to evaluate clonality of T cells.Results The 24 TCR Vβ gene repertoire displayed highly diverse and polyclonal spectratypes in all normal individuals and 4 of 8 donors. Anoth er 4 donors expressed part of the 24 TCR Vβ subfamily and 1 donor had oligoclonality. The expressions of the 24 TCR Vβ subfamilies were skewed and restric ted in 11 leukemia patients before and after transplantation. Some absences of 24 TCR Vβ subfamily expression were quite similar between the recipients pro-transplantation and related donors. The number of subfamilies expressed increased over time post-transplantation, but the restricted expressions of the subfamily could last 6-30 months after transplantation. All patients with GVHD and some without GVHD exhibited T cell clonal expansion. The expansive T cell clone was distributed in Vβ 2-3, 16-17, 18-19, 21 and Vβ 23 in patients with GVHD and in Vβ 7, 9, 16 and 19 in patients without GVHD. One patient with syngeneic-HSCT (syn-HSCT) had Vβ 15 and 16 T cell expansion after transplantation. One patient displayed Vβ 18 T cell expansion after donor lymphocyte infusion (DLI).Conclusions Normal individuals express the entire 24 TCR Vβ ge ne repertoire and have polyclonal distribution. However, the TCR Vβ gene repertoire is only partially expressed in some donors. The TCR Vβ gene repertoire is restrictedly expressed in a skew fashi展开更多
随着风电接入规模的不断攀升,多风场之间的出力相关性会对含风电机组的综合能源系统(Integrated Energy System,IES)的安全稳定运行产生越来越显著的影响。同时,考虑相关性的系统概率能流计算有助于合理地进行资源分配和优化运行方式,...随着风电接入规模的不断攀升,多风场之间的出力相关性会对含风电机组的综合能源系统(Integrated Energy System,IES)的安全稳定运行产生越来越显著的影响。同时,考虑相关性的系统概率能流计算有助于合理地进行资源分配和优化运行方式,保证系统的稳定性。文章基于相关向量机(Relevance Vector Machine,RVM)方法,提出了一种多维风电场风速的联合分布计算方法,该方法采用核函数有效避免了参数估计带来的二次误差,从而提高了计算精度。在此基础上,提出了一种基于全纯嵌入法(Holomorphic Embedding Method,HEM)的新半不变量法用于电-热联合网络的概率能流计算,相较于传统的牛顿-拉夫逊法(Newton-Raphson,NR),该算法具有更好的收敛性和计算精度。最后,采用经过修改的IEEE 33节点系统结合热力网络系统进行算例分析,进一步验证了所提算法的准确性和适用性。展开更多
The theoretical approach along with the rationale of harmonic excitation modality (HEM) applied as optimal dual controlled ventilation (DCV) to anaesthetized or severe brain injured patients, whose respiretory mechani...The theoretical approach along with the rationale of harmonic excitation modality (HEM) applied as optimal dual controlled ventilation (DCV) to anaesthetized or severe brain injured patients, whose respiretory mechanics can be properly assumed steady and linear, are presented and discussed. The design criteria of an improved version of the Advanced Lung Ventilation System (ALVS), including HEM in its functional features, are described in details. In particular, the elimination of any undesiderable artificial distortion affecting the respiratory and ventilation pattern waveforms is achieved by maintaining continuous forever the airflow inside the ventilation circuit, ensuring also the highest level of safety for patient in any condition. In such a way, the full-time compatibility of controlled breathings with spontaneous breathing activity of patient during continuous positive airways pressure (CPAP) or bilevel positive airways pressure (BiPAP) ventilation modalities and during assisted/controlled ventilation(A/CV), includeing also synchronized or triggered ventilation modalities, is an intrinsic innovative feature of the system available for clinical application. As expected and according to the clinical requirements, HEM provides for physiological respiratory and ventilation pattern waveforms together with optimal “breath to breath” feedback control of lung volume driven by an improved diagnostic measurement procedure, whose outputs are also vital for adapting all the preset ventilation parameters to the current value of the respiratory parameters of patient. The results produced by software simulations concerning both adult and neonatal patients in different clinical conditions are completely consistent with those obtained by the theoretical treatment, showing that HEM reaches the best performances from both clinical and engineering points of view.展开更多
In this paper we are presenting an intelligent method for controlling population size in evolutionary algorithms. The method uses Mediative Fuzzy Logic for modeling knowledge from experts about what should be the beha...In this paper we are presenting an intelligent method for controlling population size in evolutionary algorithms. The method uses Mediative Fuzzy Logic for modeling knowledge from experts about what should be the behavior of population size through generations based on the fitness variance and the number of generations that the algorithm is being stuck. Since, it is common that this kind of knowledge expertise can be susceptible to disagreement in a minor or a major part. We selected Mediative Fuzzy Logic (MFL) as a fuzzy method to achieve the inference. MFL is a novelty fuzzy inference method that can handle imperfect knowledge in a broader way than traditional fuzzy logic does.展开更多
To investigate the expression of the HO- 1gene in PC12 cells in hypoxic environment and gain further insight to the role of HO- 1in cerebral ischem ia,PC12 cells were exposed to hy- poxia environment(95 % N2 ,5 % CO...To investigate the expression of the HO- 1gene in PC12 cells in hypoxic environment and gain further insight to the role of HO- 1in cerebral ischem ia,PC12 cells were exposed to hy- poxia environment(95 % N2 ,5 % CO2 ) for0 .5 h,1h,4 h,8h,12 h,2 4 h respectively.The level of HO- 1m RNA was examined by reverse transcriptase polymerase chain reaction (RT- PCR) ;the volum e of COHb in the media were m easured spectrophotometrically and the c GMP concentration of PC12 cell extracts was determined by radioim munoassay.We found that after ex- posure to hypoxia for 1h,4 h,8h,12 h,2 4 h,HO- 1m RNA increased by 3% ,4 % ,17% ,31 % ,36 % as compared with that in control group respectively (P<0 .0 1or P<0 .0 5 ) ;the COHb increased by 12 % ,2 9% ,5 9% ,88% ,94 % as com pared with thatin control group respectively (P<0 .0 1or P<0 .0 5 ) ,and the c GMP concentration were2 .2 ,3.4 ,5 .2 ,8.1,10 .9- fold as that of the control group(P<0 .0 1) .We are led to conclude that hypoxia induced the expression of HO- 1gene,the production of endogenous CO,and the concentration of c GMP was elevated as well.展开更多
This paper presents a simulation and modeling package based on Matlab for a parallel hybrid electric motorcycle (HEM). The package consists of several main detailed models: internal combustion engine (ICE), motor, con...This paper presents a simulation and modeling package based on Matlab for a parallel hybrid electric motorcycle (HEM). The package consists of several main detailed models: internal combustion engine (ICE), motor, continuously variable transmission (CVT), battery, energy management system (EMS) etc . Each component is built as a library, and can be connected together according to the parallel HEM's topology. Simulation results, such as ICE power demand, motor power demand, battery instantaneous state of charge (SOC), pollution emissions etc. are given and discussed. Lastly experimental data verify our simulation results.展开更多
基金ThisstudywassupportedbytheNationalNatureScienceFoundationofChina (NO 3 9970 70 6)
文摘Background We distinguished graft-versus-host disease (GVHD) from graft-versus-leukemia (GVL) effects and to investigate the distribution of T-cell receptor (TCR) Vβ gene repertoire in individuals with leukemia before and after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods Peripheral blood mononuclear cells (PBMC) were obtained from 10 normal individuals, 8 donors and 11 patients with leukemia before and after transplantation. Polymerase chain reaction (PCR) amplification of complementarity-determining region 3 (CDR3) of 24 TCR Vβ genes was used to examine serial samples of PBMC. The PCR products were further analyzed by genescan to evaluate clonality of T cells.Results The 24 TCR Vβ gene repertoire displayed highly diverse and polyclonal spectratypes in all normal individuals and 4 of 8 donors. Anoth er 4 donors expressed part of the 24 TCR Vβ subfamily and 1 donor had oligoclonality. The expressions of the 24 TCR Vβ subfamilies were skewed and restric ted in 11 leukemia patients before and after transplantation. Some absences of 24 TCR Vβ subfamily expression were quite similar between the recipients pro-transplantation and related donors. The number of subfamilies expressed increased over time post-transplantation, but the restricted expressions of the subfamily could last 6-30 months after transplantation. All patients with GVHD and some without GVHD exhibited T cell clonal expansion. The expansive T cell clone was distributed in Vβ 2-3, 16-17, 18-19, 21 and Vβ 23 in patients with GVHD and in Vβ 7, 9, 16 and 19 in patients without GVHD. One patient with syngeneic-HSCT (syn-HSCT) had Vβ 15 and 16 T cell expansion after transplantation. One patient displayed Vβ 18 T cell expansion after donor lymphocyte infusion (DLI).Conclusions Normal individuals express the entire 24 TCR Vβ ge ne repertoire and have polyclonal distribution. However, the TCR Vβ gene repertoire is only partially expressed in some donors. The TCR Vβ gene repertoire is restrictedly expressed in a skew fashi
文摘The theoretical approach along with the rationale of harmonic excitation modality (HEM) applied as optimal dual controlled ventilation (DCV) to anaesthetized or severe brain injured patients, whose respiretory mechanics can be properly assumed steady and linear, are presented and discussed. The design criteria of an improved version of the Advanced Lung Ventilation System (ALVS), including HEM in its functional features, are described in details. In particular, the elimination of any undesiderable artificial distortion affecting the respiratory and ventilation pattern waveforms is achieved by maintaining continuous forever the airflow inside the ventilation circuit, ensuring also the highest level of safety for patient in any condition. In such a way, the full-time compatibility of controlled breathings with spontaneous breathing activity of patient during continuous positive airways pressure (CPAP) or bilevel positive airways pressure (BiPAP) ventilation modalities and during assisted/controlled ventilation(A/CV), includeing also synchronized or triggered ventilation modalities, is an intrinsic innovative feature of the system available for clinical application. As expected and according to the clinical requirements, HEM provides for physiological respiratory and ventilation pattern waveforms together with optimal “breath to breath” feedback control of lung volume driven by an improved diagnostic measurement procedure, whose outputs are also vital for adapting all the preset ventilation parameters to the current value of the respiratory parameters of patient. The results produced by software simulations concerning both adult and neonatal patients in different clinical conditions are completely consistent with those obtained by the theoretical treatment, showing that HEM reaches the best performances from both clinical and engineering points of view.
文摘In this paper we are presenting an intelligent method for controlling population size in evolutionary algorithms. The method uses Mediative Fuzzy Logic for modeling knowledge from experts about what should be the behavior of population size through generations based on the fitness variance and the number of generations that the algorithm is being stuck. Since, it is common that this kind of knowledge expertise can be susceptible to disagreement in a minor or a major part. We selected Mediative Fuzzy Logic (MFL) as a fuzzy method to achieve the inference. MFL is a novelty fuzzy inference method that can handle imperfect knowledge in a broader way than traditional fuzzy logic does.
文摘To investigate the expression of the HO- 1gene in PC12 cells in hypoxic environment and gain further insight to the role of HO- 1in cerebral ischem ia,PC12 cells were exposed to hy- poxia environment(95 % N2 ,5 % CO2 ) for0 .5 h,1h,4 h,8h,12 h,2 4 h respectively.The level of HO- 1m RNA was examined by reverse transcriptase polymerase chain reaction (RT- PCR) ;the volum e of COHb in the media were m easured spectrophotometrically and the c GMP concentration of PC12 cell extracts was determined by radioim munoassay.We found that after ex- posure to hypoxia for 1h,4 h,8h,12 h,2 4 h,HO- 1m RNA increased by 3% ,4 % ,17% ,31 % ,36 % as compared with that in control group respectively (P<0 .0 1or P<0 .0 5 ) ;the COHb increased by 12 % ,2 9% ,5 9% ,88% ,94 % as com pared with thatin control group respectively (P<0 .0 1or P<0 .0 5 ) ,and the c GMP concentration were2 .2 ,3.4 ,5 .2 ,8.1,10 .9- fold as that of the control group(P<0 .0 1) .We are led to conclude that hypoxia induced the expression of HO- 1gene,the production of endogenous CO,and the concentration of c GMP was elevated as well.
文摘This paper presents a simulation and modeling package based on Matlab for a parallel hybrid electric motorcycle (HEM). The package consists of several main detailed models: internal combustion engine (ICE), motor, continuously variable transmission (CVT), battery, energy management system (EMS) etc . Each component is built as a library, and can be connected together according to the parallel HEM's topology. Simulation results, such as ICE power demand, motor power demand, battery instantaneous state of charge (SOC), pollution emissions etc. are given and discussed. Lastly experimental data verify our simulation results.
